Combined actions of Pb2+, Zn2+, and Al3+ on voltage-activated calcium channel currents

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

1. Whole-cell patch clamp experiments were performed on rat dorsal root ganglion (DRG) neurons to investigate the actions of various combinations of Pb2+, Zn2+, and Al3+ on voltage-activated calcium channel currents (VACCCs). 2. Each of these metals has been shown to reduce VACCCs. 3. We investigated the effects of simultaneous application of two cations in the range of their IC50 values. For all possible combinations (Pb2+/Zu2+, Zn2+/Al3+, Al3+/Pb2+), independent of the order of application, we found additive actions on VACCCs. 4. We observed a 75% (±9%) block of the control current when two cations were applied simultaneously. This observation is consistent with both, an action of two metals at the same site as well as independent actions at different locations of the ion channel. 5. The additivity of the effects should be taken into account for questions of public health and the assessment of threshold limits in cases of environmental contamination.

Original languageEnglish
Pages (from-to)831-840
Number of pages10
JournalCellular and Molecular Neurobiology
Volume14
Issue number6
DOIs
Publication statusPublished - Dec 1994
Externally publishedYes

Fingerprint

Calcium Channels
Cations
Electric potential
Metals
Clamping devices
Spinal Ganglia
Electric current control
Public health
Ion Channels
Inhibitory Concentration 50
Neurons
Rats
Contamination
Public Health
Experiments

Keywords

  • aluminum (Al)
  • lead (Pb)
  • metal neurotoxicity
  • voltage-activated calcium channels
  • zinc (Zn)

ASJC Scopus subject areas

  • Neuroscience(all)
  • Genetics
  • Clinical Biochemistry
  • Cell Biology

Cite this

Combined actions of Pb2+, Zn2+, and Al3+ on voltage-activated calcium channel currents. / Platt, B.; Busselberg, Dietrich.

In: Cellular and Molecular Neurobiology, Vol. 14, No. 6, 12.1994, p. 831-840.

Research output: Contribution to journalArticle

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N2 - 1. Whole-cell patch clamp experiments were performed on rat dorsal root ganglion (DRG) neurons to investigate the actions of various combinations of Pb2+, Zn2+, and Al3+ on voltage-activated calcium channel currents (VACCCs). 2. Each of these metals has been shown to reduce VACCCs. 3. We investigated the effects of simultaneous application of two cations in the range of their IC50 values. For all possible combinations (Pb2+/Zu2+, Zn2+/Al3+, Al3+/Pb2+), independent of the order of application, we found additive actions on VACCCs. 4. We observed a 75% (±9%) block of the control current when two cations were applied simultaneously. This observation is consistent with both, an action of two metals at the same site as well as independent actions at different locations of the ion channel. 5. The additivity of the effects should be taken into account for questions of public health and the assessment of threshold limits in cases of environmental contamination.

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