Collagenase from rabbit pulmonary alveolar macrophages

Allen L. Horwitz, Ronald G. Crystal

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Rabbit pulmonary alveolar macrophages produce a collagenase which lyses labeled collagen gels, specifically cleaves collagen types I, II and III, is inhibited by ethylenediaminetetraacetate, cysteine, dithiothreitol and serum but is not inhibited by a serine protease inhibitor. Alveolar macrophage collagenase activity can be enhanced by in vivo BCG activation, in vitro latex, silica or mycobacterium activation and by in vitro uncovering of latent enzymatic activity with trypsin treatment. The production of collagenase by unactivated alveolar macrophages and the presence of "latent" collagenase in culture media of alveolar macrophages are examples of significant differences between alveolar and peritoneal macrophages.

Original languageEnglish
Pages (from-to)296-303
Number of pages8
JournalBiochemical and Biophysical Research Communications
Issue number2
Publication statusPublished - 22 Mar 1976


ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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