Collagenase from rabbit pulmonary alveolar macrophages

Allen L. Horwitz, Ronald Crystal

Research output: Contribution to journalArticle

42 Citations (Scopus)

Abstract

Rabbit pulmonary alveolar macrophages produce a collagenase which lyses labeled collagen gels, specifically cleaves collagen types I, II and III, is inhibited by ethylenediaminetetraacetate, cysteine, dithiothreitol and serum but is not inhibited by a serine protease inhibitor. Alveolar macrophage collagenase activity can be enhanced by in vivo BCG activation, in vitro latex, silica or mycobacterium activation and by in vitro uncovering of latent enzymatic activity with trypsin treatment. The production of collagenase by unactivated alveolar macrophages and the presence of "latent" collagenase in culture media of alveolar macrophages are examples of significant differences between alveolar and peritoneal macrophages.

Original languageEnglish
Pages (from-to)296-303
Number of pages8
JournalBiochemical and Biophysical Research Communications
Volume69
Issue number2
DOIs
Publication statusPublished - 22 Mar 1976
Externally publishedYes

Fingerprint

Alveolar Macrophages
Collagenases
Rabbits
Chemical activation
Serine Proteinase Inhibitors
Macrophages
Dithiothreitol
Latex
Collagen Type I
Silicon Dioxide
Trypsin
Cysteine
Culture Media
Collagen Type III
Collagen
Collagen Type II
Gels
Peritoneal Macrophages
Mycobacterium
Mycobacterium bovis

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Collagenase from rabbit pulmonary alveolar macrophages. / Horwitz, Allen L.; Crystal, Ronald.

In: Biochemical and Biophysical Research Communications, Vol. 69, No. 2, 22.03.1976, p. 296-303.

Research output: Contribution to journalArticle

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