Collagen specific immunity mediated in vitro by T lymphocytes from patients with pulmonary fibrosis

T. C. Kravis, A. Ahmed, Ronald Crystal

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Pheripheral Blood Lymphocytes (106/ml) from each of 24 patients with pulmonary fibrosis were cultured with either purified lung collagen, α 1 or α 2 chains (each at 25μg/ml). After 24-48 hours at 37°C lymphocytes of 22/24 patients produced Macrophage Inhibitory Factor (MIF) active against guinea pig peritoneal macrophages (1.5 x 106/ml) in response to collagen (31 ± 6% inhibition), 5/5 in response to α 2 chains (32 ± 5%) and 4/5 to α 1 chains (23± 5% inhibition). Peripheral Blood Lymphocytes from 15 normal volunteers and 25/27 patients with non fibrotic lung diseases failed to produce significant MIF (10 ± 6%) in response to collagen or its constituent chains. Pheripheral Blood Lymphocytes from 12/12 patients with pulmonary fibrosis specifically lysed collagen coated sheep erythrocytes while those from 15 normal volunteers did not. Treatment of the blood lymphocytes from patients with pulmonary fibrosis with Complement and a monospecific antibody to Human Thymic Lymphocyte Antigen abrogated both the production of MIF and the mediation of specific cytolysis. These studies suggest that T cell immunity to collagen is associated with pulmonary fibrosis.

Original languageEnglish
Number of pages1
JournalAmerican Review of Respiratory Disease
Issue number4 II
Publication statusPublished - 1 Jan 1976
Externally publishedYes


ASJC Scopus subject areas

  • Pulmonary and Respiratory Medicine

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