Abstract
The human HIRA gene was identified as a putative transcriptional regulator mapping within the Di-George syndrome critical region at 22q11. HIRA-related proteins have been described in a number of species, but functional information concerning family members is only available in Saccharomyces cerevisiae, where the Hir1p and Hir2p proteins are known to be transcriptional corepressors. In order to analyse conservation of HIRA-related genes and to provide resources for functional studies in another model organism we have isolated the HIRA gene from Drosophila melanogaster (dhira). The 3374 nucleotide cDNA encodes a protein of 1047 aa, showing 42% identity with the human protein. Alignment with the predicted HIRA proteins from human, mouse, chick and pufferfish reveals strong conservation within the N-terminal region which contains seven WD domains, with less conservation of C-terminal sequences. In situ hybridisation to salivary gland chromosomes indicates that the gene resides in region 7B2-3 of the X chromosome. Dhira is expressed through embryonic development and at lower levels during larval and pupal development. The expression of dhira is dramatically increased in early embryos and in females, suggesting that the dhira mRNA could be maternally deposited in the embryos.
| Original language | English |
|---|---|
| Pages (from-to) | 486-491 |
| Number of pages | 6 |
| Journal | Biochemical and Biophysical Research Communications |
| Volume | 249 |
| Issue number | 2 |
| DOIs | |
| Publication status | Published - 19 Aug 1998 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Biochemistry
- Biophysics
- Molecular Biology
Cite this
Cloning, chromosome mapping and expression analysis of the HIRA gene from Drosophila melanogaster. / Llevadot, Roser; Marqués, Guillermo; Pritchard, Melanie; Estivill, Xavier P.; Ferrús, Alberto; Scambler, Peter.
In: Biochemical and Biophysical Research Communications, Vol. 249, No. 2, 19.08.1998, p. 486-491.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Cloning, chromosome mapping and expression analysis of the HIRA gene from Drosophila melanogaster
AU - Llevadot, Roser
AU - Marqués, Guillermo
AU - Pritchard, Melanie
AU - Estivill, Xavier P.
AU - Ferrús, Alberto
AU - Scambler, Peter
PY - 1998/8/19
Y1 - 1998/8/19
N2 - The human HIRA gene was identified as a putative transcriptional regulator mapping within the Di-George syndrome critical region at 22q11. HIRA-related proteins have been described in a number of species, but functional information concerning family members is only available in Saccharomyces cerevisiae, where the Hir1p and Hir2p proteins are known to be transcriptional corepressors. In order to analyse conservation of HIRA-related genes and to provide resources for functional studies in another model organism we have isolated the HIRA gene from Drosophila melanogaster (dhira). The 3374 nucleotide cDNA encodes a protein of 1047 aa, showing 42% identity with the human protein. Alignment with the predicted HIRA proteins from human, mouse, chick and pufferfish reveals strong conservation within the N-terminal region which contains seven WD domains, with less conservation of C-terminal sequences. In situ hybridisation to salivary gland chromosomes indicates that the gene resides in region 7B2-3 of the X chromosome. Dhira is expressed through embryonic development and at lower levels during larval and pupal development. The expression of dhira is dramatically increased in early embryos and in females, suggesting that the dhira mRNA could be maternally deposited in the embryos.
AB - The human HIRA gene was identified as a putative transcriptional regulator mapping within the Di-George syndrome critical region at 22q11. HIRA-related proteins have been described in a number of species, but functional information concerning family members is only available in Saccharomyces cerevisiae, where the Hir1p and Hir2p proteins are known to be transcriptional corepressors. In order to analyse conservation of HIRA-related genes and to provide resources for functional studies in another model organism we have isolated the HIRA gene from Drosophila melanogaster (dhira). The 3374 nucleotide cDNA encodes a protein of 1047 aa, showing 42% identity with the human protein. Alignment with the predicted HIRA proteins from human, mouse, chick and pufferfish reveals strong conservation within the N-terminal region which contains seven WD domains, with less conservation of C-terminal sequences. In situ hybridisation to salivary gland chromosomes indicates that the gene resides in region 7B2-3 of the X chromosome. Dhira is expressed through embryonic development and at lower levels during larval and pupal development. The expression of dhira is dramatically increased in early embryos and in females, suggesting that the dhira mRNA could be maternally deposited in the embryos.
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UR - http://www.scopus.com/inward/citedby.url?scp=0032547290&partnerID=8YFLogxK
U2 - 10.1006/bbrc.1998.9165
DO - 10.1006/bbrc.1998.9165
M3 - Article
C2 - 9712723
AN - SCOPUS:0032547290
VL - 249
SP - 486
EP - 491
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
SN - 0006-291X
IS - 2
ER -