Cloning (CAG/GTC)n STSs by an Alu-(CAG/GTC)n PCR method: An approach to human chromosome 12 and spinocerebellar ataxia 2 (SCA2)

M. A. Pujana, V. Volpini, X. Estivill

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Abstract

We report here an Alu-(CAG/GTC)n PCR method for the cloning of STSs with (CAG/GTC)n sequences. We have applied this method to genomic DNA of a somatic cell hybrid containing human chromosome 12 where linkage has been found for a known familiar dominant ataxia (SCA2), which is thought to be due to a (CAG/GTC)n expansion. We have isolated several clones containing (CAG/GTC)n sequences, which include previously identified sequences that map to chromosome 12. This method could be a new PCR approach for the cloning of repeats based on their proximity to Alu sequences.

Original languageEnglish
Pages (from-to)3651-3652
Number of pages2
JournalNucleic acids research
Volume24
Issue number18
Publication statusPublished - 1 Oct 1996

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ASJC Scopus subject areas

  • Genetics

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