Cloning (CAG/GTC)n STSs by an Alu-(CAG/GTC)n PCR method

An approach to human chromosome 12 and spinocerebellar ataxia 2 (SCA2)

M. A. Pujana, V. Volpini, Xavier P. Estivill

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

We report here an Alu-(CAG/GTC)n PCR method for the cloning of STSs with (CAG/GTC)n sequences. We have applied this method to genomic DNA of a somatic cell hybrid containing human chromosome 12 where linkage has been found for a known familiar dominant ataxia (SCA2), which is thought to be due to a (CAG/GTC)n expansion. We have isolated several clones containing (CAG/GTC)n sequences, which include previously identified sequences that map to chromosome 12. This method could be a new PCR approach for the cloning of repeats based on their proximity to Alu sequences.

Original languageEnglish
Pages (from-to)3651-3652
Number of pages2
JournalNucleic Acids Research
Volume24
Issue number18
Publication statusPublished - 1996
Externally publishedYes

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Spinocerebellar Ataxias
Chromosomes, Human, Pair 12
Human Chromosomes
Organism Cloning
Polymerase Chain Reaction
Hybrid Cells
Ataxia
Clone Cells
DNA

ASJC Scopus subject areas

  • Genetics

Cite this

Cloning (CAG/GTC)n STSs by an Alu-(CAG/GTC)n PCR method : An approach to human chromosome 12 and spinocerebellar ataxia 2 (SCA2). / Pujana, M. A.; Volpini, V.; Estivill, Xavier P.

In: Nucleic Acids Research, Vol. 24, No. 18, 1996, p. 3651-3652.

Research output: Contribution to journalArticle

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