Cloning and characterization of DYRK1B, a novel member of the DYRK family of protein kinases

Susanne Leder, Yvonne Weber, Xavier Altafaj, Xavier P. Estivill, Hans Georg Joost, Walter Becker

Research output: Contribution to journalArticle

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Abstract

The DYRK1A gene on human chromosome 21 encodes a protein kinase presumed to be involved in the pathogenesis of mental retardation in Down's syndrome. Here we describe a highly similar homolog, DYRK1B, which is, in contrast to DYRK1A, predominately expressed in muscle and testis. The human DYRK1B gene was mapped to chromosome 19 (19q12-13.11) by radiation hybrid analysis. The amino acid sequences of DYRK1A and DYRK1B are 84% identical in the N-terminus and the catalytic domain but show no extended sequence similarity in the C-terminal region. DYRK1B contains all motifs characteristic for the DYRK family of protein kinases. In addition, the sequence comprises a bipartite nuclear localization motif. A green fluorescent protein (GFP) fusion protein of DYRK1B was found mainly in the nucleus of transfected COS-7 cells. These data suggest that DYRK1B is a muscle- and testis-specific isoform of DYRK1A and is involved in the regulation of nuclear functions.

Original languageEnglish
Pages (from-to)474-479
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume254
Issue number2
DOIs
Publication statusPublished - 19 Jan 1999
Externally publishedYes

Fingerprint

Cloning
Chromosomes
Protein Kinases
Muscle
Testis
Organism Cloning
Genes
Radiation Hybrid Mapping
Chromosomes, Human, Pair 19
Chromosomes, Human, Pair 21
Muscles
COS Cells
Human Chromosomes
Down Syndrome
Green Fluorescent Proteins
Intellectual Disability
Amino Acid Sequence
Catalytic Domain
Protein Isoforms
Fusion reactions

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Cloning and characterization of DYRK1B, a novel member of the DYRK family of protein kinases. / Leder, Susanne; Weber, Yvonne; Altafaj, Xavier; Estivill, Xavier P.; Joost, Hans Georg; Becker, Walter.

In: Biochemical and Biophysical Research Communications, Vol. 254, No. 2, 19.01.1999, p. 474-479.

Research output: Contribution to journalArticle

Leder, Susanne ; Weber, Yvonne ; Altafaj, Xavier ; Estivill, Xavier P. ; Joost, Hans Georg ; Becker, Walter. / Cloning and characterization of DYRK1B, a novel member of the DYRK family of protein kinases. In: Biochemical and Biophysical Research Communications. 1999 ; Vol. 254, No. 2. pp. 474-479.
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