Cloning and characterization of DYRK1B, a novel member of the DYRK family of protein kinases

Susanne Leder, Yvonne Weber, Xavier Altafaj, Xavier Estivill, Hans Georg Joost, Walter Becker

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53 Citations (Scopus)

Abstract

The DYRK1A gene on human chromosome 21 encodes a protein kinase presumed to be involved in the pathogenesis of mental retardation in Down's syndrome. Here we describe a highly similar homolog, DYRK1B, which is, in contrast to DYRK1A, predominately expressed in muscle and testis. The human DYRK1B gene was mapped to chromosome 19 (19q12-13.11) by radiation hybrid analysis. The amino acid sequences of DYRK1A and DYRK1B are 84% identical in the N-terminus and the catalytic domain but show no extended sequence similarity in the C-terminal region. DYRK1B contains all motifs characteristic for the DYRK family of protein kinases. In addition, the sequence comprises a bipartite nuclear localization motif. A green fluorescent protein (GFP) fusion protein of DYRK1B was found mainly in the nucleus of transfected COS-7 cells. These data suggest that DYRK1B is a muscle- and testis-specific isoform of DYRK1A and is involved in the regulation of nuclear functions.

Original languageEnglish
Pages (from-to)474-479
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume254
Issue number2
DOIs
Publication statusPublished - 19 Jan 1999

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ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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