Cloning and characterisation of a Plasmodium falciparum homologue of the Ran/TC4 signal transducing GTPase involved in cell cycle control

Ali Sultan, William A. Richardson, Pietro Alano, David E. Arnot, Christian Doerig

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

On the basis of conserved sequences characteristic of the Ran/TC4 subfamily of the GTPase superfamily a fragment of the gene encoding a Plasmodium falciparum Ran/TC4 homologue was amplified in the polymerase chain reaction. The fragment was used to screen a cDNA library to obtain clones which allowed determination of the complete gene sequence. The gene designated pfran (Plasmodium falciparum ras-like nuclear protein) has around 70% amino acid identity with previously characterised Ran/TC4 proteins. Like other malarial mRNAs the pfran mRNA contains a long (at least 679 bp) 5′ untranslated region. Southern blotting experiments show that pfran is a single copy gene located on chromosome 11. RNA hybridisation experiments indicate that pfran mRNA is abundant in late trophozoite and schizont stages but present at very low levels in gametocytes and early asexual stages.

Original languageEnglish
Pages (from-to)331-338
Number of pages8
JournalMolecular and Biochemical Parasitology
Volume65
Issue number2
DOIs
Publication statusPublished - 1994
Externally publishedYes

Fingerprint

GTP Phosphohydrolases
Plasmodium falciparum
Cell Cycle Checkpoints
Organism Cloning
Nuclear Proteins
ran GTP-Binding Protein
Messenger RNA
Genes
Schizonts
Trophozoites
Chromosomes, Human, Pair 11
Conserved Sequence
5' Untranslated Regions
Southern Blotting
Gene Library
Clone Cells
RNA
Amino Acids
Polymerase Chain Reaction

Keywords

  • Cell cycle
  • GTPase
  • pfran
  • Plasmodium falciparum

ASJC Scopus subject areas

  • Molecular Biology
  • Parasitology

Cite this

Cloning and characterisation of a Plasmodium falciparum homologue of the Ran/TC4 signal transducing GTPase involved in cell cycle control. / Sultan, Ali; Richardson, William A.; Alano, Pietro; Arnot, David E.; Doerig, Christian.

In: Molecular and Biochemical Parasitology, Vol. 65, No. 2, 1994, p. 331-338.

Research output: Contribution to journalArticle

Sultan, Ali ; Richardson, William A. ; Alano, Pietro ; Arnot, David E. ; Doerig, Christian. / Cloning and characterisation of a Plasmodium falciparum homologue of the Ran/TC4 signal transducing GTPase involved in cell cycle control. In: Molecular and Biochemical Parasitology. 1994 ; Vol. 65, No. 2. pp. 331-338.
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