Clinical application of molecular biology

A study of allograft rejection with polymerase chain reaction

Research output: Contribution to journalArticle

44 Citations (Scopus)

Abstract

This article explores the clinical usefulness of reverse transcriptase- polymerase chain reaction in organ graft recipients. In this study, reverse transcriptase-polymerase chain reaction was used to identify intrarenal expression of cytotoxic attack molecules (granzyme B and perforin) and immunoregulatory cytokines (IL-2, IL-4, IL-10, IFN-γ, and TGF-β1) in human renal allograft biopsies. The biopsies (n = 127) were classified using the Banff criteria, and intrarenal gene expression was correlated with the histologic diagnosis. Molecular analyses revealed that intragraft display of mRNA encoding granzyme B, IL-10, or IL-2 is a correlate of acute rejection, and intrarenal expression of TGFβ1 mRNA is a correlate of chronic rejection. In addition to demonstrating differential and highly selective intragraft gene expression during rejection, these data suggest that therapeutic strategies directed at the molecular correlates of rejection might refine existing antirejection strategies.

Original languageEnglish
Pages (from-to)264-267
Number of pages4
JournalAmerican Journal of the Medical Sciences
Volume313
Issue number5
Publication statusPublished - 1 Jan 1997
Externally publishedYes

Fingerprint

Reverse Transcriptase Polymerase Chain Reaction
Interleukin-10
Interleukin-2
Allografts
Molecular Biology
Biopsy
Gene Expression
Granzymes
Polymerase Chain Reaction
Messenger RNA
Interleukin-4
Cytokines
Transplants
Kidney
Therapeutics
perforin-granzyme B

Keywords

  • Gene expression
  • Rejection
  • Renal allograft

ASJC Scopus subject areas

  • Medicine(all)

Cite this

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abstract = "This article explores the clinical usefulness of reverse transcriptase- polymerase chain reaction in organ graft recipients. In this study, reverse transcriptase-polymerase chain reaction was used to identify intrarenal expression of cytotoxic attack molecules (granzyme B and perforin) and immunoregulatory cytokines (IL-2, IL-4, IL-10, IFN-γ, and TGF-β1) in human renal allograft biopsies. The biopsies (n = 127) were classified using the Banff criteria, and intrarenal gene expression was correlated with the histologic diagnosis. Molecular analyses revealed that intragraft display of mRNA encoding granzyme B, IL-10, or IL-2 is a correlate of acute rejection, and intrarenal expression of TGFβ1 mRNA is a correlate of chronic rejection. In addition to demonstrating differential and highly selective intragraft gene expression during rejection, these data suggest that therapeutic strategies directed at the molecular correlates of rejection might refine existing antirejection strategies.",
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