Cigarette smoking induces small airway epithelial epigenetic changes with corresponding modulation of gene expression

Lauren J. Buro-Auriemma, Jacqueline Salit, Neil R. Hackett, Matthew S. Walters, Yael Strulovici-Barel, Michelle R. Staudt, Jennifer Fuller, Mai Mahmoud, Christopher S. Stevenson, Holly Hilton, Melisa W.Y. Ho, Ronald Crystal

Research output: Contribution to journalArticle

53 Citations (Scopus)

Abstract

The small airway epithelium (SAE), the first site of smoking-induced lung pathology, exhibits genome-wide changes in geneexpression in response to cigarettesmoking.Basedonthe increasing evidence that the epigenome can respond to external stimuli in a rapid manner, we assessed the SAE of smokers for genome-wide DNA methylation changes compared with nonsmokers, and whether changes inSAEDNAmethylation were linked to the transcriptional output of these cells. Using genome-wide methylation analysis of SAE DNA of nonsmokers and smokers, the data identified 204 unique genes differentially methylated in SAE DNA of smokers compared withnonsmokers, with67%of the regions with differentialmethylation occurring within 2 kbof the transcriptional start site. Among the genes with differential methylation were those related to metabolism, transcription, signal transduction and transport. For the differentially methylated genes, 35 exhibited a correlation with gene expression, 54% with an inverse correlation of DNA methylation with gene expression and 46% a direct correlation. These observations provide evidence that cigarette smoking alters the DNA methylation patterning of the SAE and that, for some genes, these changes are associated with the smoking-related changes in gene expression.

Original languageEnglish
Article numberddt326
Pages (from-to)4726-4738
Number of pages13
JournalHuman Molecular Genetics
Volume22
Issue number23
DOIs
Publication statusPublished - 1 Dec 2013
Externally publishedYes

Fingerprint

Epigenomics
Epithelium
Smoking
Gene Expression
DNA Methylation
Genome
Methylation
Genes
DNA
Signal Transduction
Pathology
Lung

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Genetics(clinical)

Cite this

Buro-Auriemma, L. J., Salit, J., Hackett, N. R., Walters, M. S., Strulovici-Barel, Y., Staudt, M. R., ... Crystal, R. (2013). Cigarette smoking induces small airway epithelial epigenetic changes with corresponding modulation of gene expression. Human Molecular Genetics, 22(23), 4726-4738. [ddt326]. https://doi.org/10.1093/hmg/ddt326

Cigarette smoking induces small airway epithelial epigenetic changes with corresponding modulation of gene expression. / Buro-Auriemma, Lauren J.; Salit, Jacqueline; Hackett, Neil R.; Walters, Matthew S.; Strulovici-Barel, Yael; Staudt, Michelle R.; Fuller, Jennifer; Mahmoud, Mai; Stevenson, Christopher S.; Hilton, Holly; Ho, Melisa W.Y.; Crystal, Ronald.

In: Human Molecular Genetics, Vol. 22, No. 23, ddt326, 01.12.2013, p. 4726-4738.

Research output: Contribution to journalArticle

Buro-Auriemma, LJ, Salit, J, Hackett, NR, Walters, MS, Strulovici-Barel, Y, Staudt, MR, Fuller, J, Mahmoud, M, Stevenson, CS, Hilton, H, Ho, MWY & Crystal, R 2013, 'Cigarette smoking induces small airway epithelial epigenetic changes with corresponding modulation of gene expression', Human Molecular Genetics, vol. 22, no. 23, ddt326, pp. 4726-4738. https://doi.org/10.1093/hmg/ddt326
Buro-Auriemma LJ, Salit J, Hackett NR, Walters MS, Strulovici-Barel Y, Staudt MR et al. Cigarette smoking induces small airway epithelial epigenetic changes with corresponding modulation of gene expression. Human Molecular Genetics. 2013 Dec 1;22(23):4726-4738. ddt326. https://doi.org/10.1093/hmg/ddt326
Buro-Auriemma, Lauren J. ; Salit, Jacqueline ; Hackett, Neil R. ; Walters, Matthew S. ; Strulovici-Barel, Yael ; Staudt, Michelle R. ; Fuller, Jennifer ; Mahmoud, Mai ; Stevenson, Christopher S. ; Hilton, Holly ; Ho, Melisa W.Y. ; Crystal, Ronald. / Cigarette smoking induces small airway epithelial epigenetic changes with corresponding modulation of gene expression. In: Human Molecular Genetics. 2013 ; Vol. 22, No. 23. pp. 4726-4738.
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