Chromatin Transitions during Early Xenopus Embryogenesis

Changes in Histone H4 Acetylation and in Linker Histone Type

Stefan Dimitrov, Geneviève Almouzni, Mary Dasso, Alan P. Wolffe

Research output: Contribution to journalArticle

154 Citations (Scopus)

Abstract

We describe major transitions in the type and modification of chromatin-associated proteins during the early development of Xenopus laevis. Histone H4 is stored in the diacetylated form in the egg and is progressively deacetylated during normal development. If histone deacetylases are inhibited with sodium butyrate, hyperacetylated histone H4 only accumulates after the mid-blastula transition. The type of linker histone in chromatin also changes during embryogenesis, from predominantly the B4 protein at the mid-blastula transition to predominantly histone H1 at the end of gastrulation. These transitions in chromatin composition correlate with major changes in the replicative and transcriptional activity of embryonic nuclei.

Original languageEnglish
Pages (from-to)214-227
Number of pages14
JournalDevelopmental Biology
Volume160
Issue number1
DOIs
Publication statusPublished - Nov 1993
Externally publishedYes

Fingerprint

Acetylation
Xenopus
Histones
Chromatin
Embryonic Development
Blastula
Gastrulation
Histone Deacetylases
Butyric Acid
Xenopus laevis
Ovum
Proteins

ASJC Scopus subject areas

  • Molecular Biology
  • Developmental Biology
  • Cell Biology

Cite this

Chromatin Transitions during Early Xenopus Embryogenesis : Changes in Histone H4 Acetylation and in Linker Histone Type. / Dimitrov, Stefan; Almouzni, Geneviève; Dasso, Mary; Wolffe, Alan P.

In: Developmental Biology, Vol. 160, No. 1, 11.1993, p. 214-227.

Research output: Contribution to journalArticle

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