Characterization of drug resistance and genetic diversity of Plasmodium falciparum parasites from Tripura, Northeast India

S. J. Patgiri, K. Sarma, N. Sarmah, N. Bhattacharyya, D. K. Sarma, T. Nirmolia, D. R. Bhattacharyya, P. K. Mohapatra, Devendra Bansal, P. K. Bharti, R. Sehgal, J. Mahanta, A. A. Sultan

Research output: Contribution to journalArticle

Abstract

Monitoring of anti-malarial drug resistance is vital in Northeast India as this region shares its international border with Southeast Asia. Genetic diversity of Plasmodium parasites regulates transmission dynamics, disease severity and vaccine efficacy. P. falciparum chloroquine resistance transporter (Pfcrt), multidrug resistance-1 (Pfmdr-1) and kelch 13 propeller (PfK-13) genes which govern antimalarial drug resistance and three genetic diversity markers, merozoite surface protein 1 and 2 (Pfmsp-1, Pfmsp-2) and glutamate rich protein (Pfglurp) were evaluated from Tripura, Northeast India using molecular tools. In the Pfcrt gene, 87% isolates showed triple mutations at codons M74I, N75E and K76T. 12.5% isolates in Pfmdr-1 gene showed mutation at N86Y. No polymorphism in PfK-13 propeller was found. Polyclonal infections were observed in 53.85% isolates and more commonly in adults (p = 0.0494). In the Pfmsp-1 locus, the K1 allelic family was predominant (71.2%) followed by the 3D7/IC family (69.2%) in the Pfmsp-2 locus. RII region of Pfglurp exhibited nine alleles with expected heterozygosity of 0.85. The multiplicity of infection for Pfmsp-1, Pfmsp-2 and Pfglurp were 1.56, 1.31 and 1.06 respectively. Overall, the study demonstrated a high level of chloroquine resistance and extensive parasite diversity in the region, necessitating regular surveillance in this population group.

Original languageEnglish
Number of pages1
JournalScientific reports
Volume9
Issue number1
DOIs
Publication statusPublished - 23 Sep 2019

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Plasmodium falciparum
Drug Resistance
India
Glutamic Acid
Parasites
Antimalarials
Merozoite Surface Protein 1
MDR Genes
Southeastern Asia
Mutation
Proteins
Plasmodium
Chloroquine
Multiple Drug Resistance
Infection
Genetic Markers
Codon
Genes
Vaccines
Alleles

ASJC Scopus subject areas

  • General

Cite this

Patgiri, S. J., Sarma, K., Sarmah, N., Bhattacharyya, N., Sarma, D. K., Nirmolia, T., ... Sultan, A. A. (2019). Characterization of drug resistance and genetic diversity of Plasmodium falciparum parasites from Tripura, Northeast India. Scientific reports, 9(1). https://doi.org/10.1038/s41598-019-50152-w

Characterization of drug resistance and genetic diversity of Plasmodium falciparum parasites from Tripura, Northeast India. / Patgiri, S. J.; Sarma, K.; Sarmah, N.; Bhattacharyya, N.; Sarma, D. K.; Nirmolia, T.; Bhattacharyya, D. R.; Mohapatra, P. K.; Bansal, Devendra; Bharti, P. K.; Sehgal, R.; Mahanta, J.; Sultan, A. A.

In: Scientific reports, Vol. 9, No. 1, 23.09.2019.

Research output: Contribution to journalArticle

Patgiri, SJ, Sarma, K, Sarmah, N, Bhattacharyya, N, Sarma, DK, Nirmolia, T, Bhattacharyya, DR, Mohapatra, PK, Bansal, D, Bharti, PK, Sehgal, R, Mahanta, J & Sultan, AA 2019, 'Characterization of drug resistance and genetic diversity of Plasmodium falciparum parasites from Tripura, Northeast India', Scientific reports, vol. 9, no. 1. https://doi.org/10.1038/s41598-019-50152-w
Patgiri, S. J. ; Sarma, K. ; Sarmah, N. ; Bhattacharyya, N. ; Sarma, D. K. ; Nirmolia, T. ; Bhattacharyya, D. R. ; Mohapatra, P. K. ; Bansal, Devendra ; Bharti, P. K. ; Sehgal, R. ; Mahanta, J. ; Sultan, A. A. / Characterization of drug resistance and genetic diversity of Plasmodium falciparum parasites from Tripura, Northeast India. In: Scientific reports. 2019 ; Vol. 9, No. 1.
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