Characterization of a signal peptide sequence in the cell-free translation product of sheep elastin mRNA

Jeffrey M. Davidson, Barbara Leslie, Terry Wolt, Ronald Crystal, Lawrence B. Sandberg

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

In vitro explant cultures of near-term sheep nuchal ligament secrete tropoelastin of approximate Mr 70,000-72,000 while the elastin cell-free product of sheep nuchal ligament RNA is 2000 to 3000 Mr larger. Automated Edman degradation of immunoprecipitates of radiolabeled cell-free elastin precursor demonstrated the presence of a 26-residue signal sequence which was absent from sheep tropoelastin secreted from explant cultures. In addition, a 20-residue overlap was established between the cell-free product and the secreted protein. This overlap region, representing the N-terminal sequence of ovine tropoelastin, demonstrated complete homology with the N-terminal sequence of porcine tropoelastin and near complete homology with chick tropoelastin. These findings suggest that cotranslational removal of this hydrophobic peptide extension is likely a correlate of vectorial transport of elastin into the secretory apparatus.

Original languageEnglish
Pages (from-to)31-37
Number of pages7
JournalArchives of Biochemistry and Biophysics
Volume218
Issue number1
DOIs
Publication statusPublished - 1 Oct 1982
Externally publishedYes

Fingerprint

Tropoelastin
Elastin
Protein Sorting Signals
Sheep
Messenger RNA
Ligaments
Swine
RNA
Degradation
Peptides
Proteins

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology

Cite this

Characterization of a signal peptide sequence in the cell-free translation product of sheep elastin mRNA. / Davidson, Jeffrey M.; Leslie, Barbara; Wolt, Terry; Crystal, Ronald; Sandberg, Lawrence B.

In: Archives of Biochemistry and Biophysics, Vol. 218, No. 1, 01.10.1982, p. 31-37.

Research output: Contribution to journalArticle

Davidson, Jeffrey M. ; Leslie, Barbara ; Wolt, Terry ; Crystal, Ronald ; Sandberg, Lawrence B. / Characterization of a signal peptide sequence in the cell-free translation product of sheep elastin mRNA. In: Archives of Biochemistry and Biophysics. 1982 ; Vol. 218, No. 1. pp. 31-37.
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