Characterisation of Mytilus edulis hemocyte subpopulations by single cell time-lapse motility imaging

Frank Le Foll, Damien Rioult, Sofiane Boussa, Jennifer Pasquier, Zeina Dagher, François Leboulenger

Research output: Contribution to journalArticle

34 Citations (Scopus)

Abstract

In bivalve molluscs, defence against pathogens mainly relies on fast tissue infiltration by immunocompetent hemocytes that migrate from circulating hemolymph to sites of infection, in order to deliver, in situ, an effective immune response. In the present work, we have investigated dynamics of hemocyte subpopulations motility by combining flow cytometry coupled to Coulter-type cell volume determination, Hoffman modulation contrast microscopy, time-lapse imaging and off-line analysis of cell shape changes. Our results revealed fast modifications of hemocyte aspect in vitro, with bidirectional transitions from spread outlines to condensed cell body morphologies, in the minute range. Amoeboid or non-amoeboid types of locomotion were observed, depending on the cell shapes and on the cell subtypes, with velocities reaching up to 30 μm min -1. Correlations between motion profiles, Hemacolor staining and flow cytometry analysis on living cells help to propose a functional mussel hemocyte classification including the motile properties of these cells. In particular, basophils were shown to be involved in dynamic hemocyte-hemocyte interactions and in the constitution of aggregation cores. Physiological implications, in terms of immune response in organisms devoid of endothelium-closed vascular system, and potential applications of hemocyte motility studies for the development and the interpretation of experiments involving hemocytes in the field of marine ecotoxicology are discussed.

Original languageEnglish
Pages (from-to)372-386
Number of pages15
JournalFish and Shellfish Immunology
Volume28
Issue number2
DOIs
Publication statusPublished - 1 Feb 2010
Externally publishedYes

Fingerprint

Flow cytometry
flow cytometry
motility
Mytilus edulis
immune response
hemocytes
subpopulation
Cells
image analysis
Molluscs
Imaging techniques
ecotoxicology
locomotion
Pathogens
Infiltration
mollusc
bivalve
microscopy
Microscopic examination
infiltration

Keywords

  • Dynamic microscopy
  • Hemolymph
  • Innate immunity
  • Invertebrate
  • Mussel blood cells

ASJC Scopus subject areas

  • Aquatic Science
  • Environmental Chemistry

Cite this

Characterisation of Mytilus edulis hemocyte subpopulations by single cell time-lapse motility imaging. / Le Foll, Frank; Rioult, Damien; Boussa, Sofiane; Pasquier, Jennifer; Dagher, Zeina; Leboulenger, François.

In: Fish and Shellfish Immunology, Vol. 28, No. 2, 01.02.2010, p. 372-386.

Research output: Contribution to journalArticle

Le Foll, Frank ; Rioult, Damien ; Boussa, Sofiane ; Pasquier, Jennifer ; Dagher, Zeina ; Leboulenger, François. / Characterisation of Mytilus edulis hemocyte subpopulations by single cell time-lapse motility imaging. In: Fish and Shellfish Immunology. 2010 ; Vol. 28, No. 2. pp. 372-386.
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