Cervico-Vaginal Tissue Ex Vivo as a Model to Study Early Events in HIV-1 Infection

Melanie Merbah, Andrea Introini, Wendy Fitzgerald, Jean Charles Grivel, Andrea Lisco, Christophe Vanpouille, Leonid Margolis

Research output: Contribution to journalReview article

20 Citations (Scopus)


Citation Merbah M, Introini A, Fitzgerald W, Grivel J-C, Lisco A, Vanpouille C, Margolis L. Cervico-vaginal tissue ex vivo as a model to study early events in HIV-1 infection. Am J Reprod Immunol 2011; 65: 268-278 Vaginal intercourse remains the most prevalent route of infection of women. In spite of many efforts, the detailed mechanisms of HIV-1 transmission in the female lower genital tract remain largely unknown. With all the obvious restrictions on studying these mechanisms in humans, their understanding depends on the development of adequate experimental models. Isolated cell cultures do not faithfully reproduce important aspects of cell-cell interactions in living tissues and tissue responses to pathogens. Explants and other types of ex vivo tissue models serve as a bridge between cell culture and tissues in vivo. Herein, we discuss various cervico-vaginal tissue models and their use in studying HIV vaginal transmission and consider future directions of such studies.

Original languageEnglish
Pages (from-to)268-278
Number of pages11
JournalAmerican Journal of Reproductive Immunology
Issue number3
Publication statusPublished - 1 Mar 2011



  • Cervix
  • Explants
  • HIV
  • Tissue-culture
  • Transmission

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology
  • Reproductive Medicine
  • Obstetrics and Gynaecology

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