Cervico-Vaginal Tissue Ex Vivo as a Model to Study Early Events in HIV-1 Infection

Melanie Merbah, Andrea Introini, Wendy Fitzgerald, Jean-Charles B. Grivel, Andrea Lisco, Christophe Vanpouille, Leonid Margolis

Research output: Contribution to journalReview article

20 Citations (Scopus)

Abstract

Citation Merbah M, Introini A, Fitzgerald W, Grivel J-C, Lisco A, Vanpouille C, Margolis L. Cervico-vaginal tissue ex vivo as a model to study early events in HIV-1 infection. Am J Reprod Immunol 2011; 65: 268-278 Vaginal intercourse remains the most prevalent route of infection of women. In spite of many efforts, the detailed mechanisms of HIV-1 transmission in the female lower genital tract remain largely unknown. With all the obvious restrictions on studying these mechanisms in humans, their understanding depends on the development of adequate experimental models. Isolated cell cultures do not faithfully reproduce important aspects of cell-cell interactions in living tissues and tissue responses to pathogens. Explants and other types of ex vivo tissue models serve as a bridge between cell culture and tissues in vivo. Herein, we discuss various cervico-vaginal tissue models and their use in studying HIV vaginal transmission and consider future directions of such studies.

Original languageEnglish
Pages (from-to)268-278
Number of pages11
JournalAmerican Journal of Reproductive Immunology
Volume65
Issue number3
DOIs
Publication statusPublished - Mar 2011
Externally publishedYes

Fingerprint

HIV Infections
HIV-1
Cell Culture Techniques
Cell Communication
Theoretical Models
HIV
Infection

Keywords

  • Cervix
  • Explants
  • HIV
  • Tissue-culture
  • Transmission

ASJC Scopus subject areas

  • Immunology
  • Immunology and Allergy
  • Obstetrics and Gynaecology
  • Reproductive Medicine

Cite this

Cervico-Vaginal Tissue Ex Vivo as a Model to Study Early Events in HIV-1 Infection. / Merbah, Melanie; Introini, Andrea; Fitzgerald, Wendy; Grivel, Jean-Charles B.; Lisco, Andrea; Vanpouille, Christophe; Margolis, Leonid.

In: American Journal of Reproductive Immunology, Vol. 65, No. 3, 03.2011, p. 268-278.

Research output: Contribution to journalReview article

Merbah, Melanie ; Introini, Andrea ; Fitzgerald, Wendy ; Grivel, Jean-Charles B. ; Lisco, Andrea ; Vanpouille, Christophe ; Margolis, Leonid. / Cervico-Vaginal Tissue Ex Vivo as a Model to Study Early Events in HIV-1 Infection. In: American Journal of Reproductive Immunology. 2011 ; Vol. 65, No. 3. pp. 268-278.
@article{73ede0fbbc3d4cff8f2c046f9b5dab8c,
title = "Cervico-Vaginal Tissue Ex Vivo as a Model to Study Early Events in HIV-1 Infection",
abstract = "Citation Merbah M, Introini A, Fitzgerald W, Grivel J-C, Lisco A, Vanpouille C, Margolis L. Cervico-vaginal tissue ex vivo as a model to study early events in HIV-1 infection. Am J Reprod Immunol 2011; 65: 268-278 Vaginal intercourse remains the most prevalent route of infection of women. In spite of many efforts, the detailed mechanisms of HIV-1 transmission in the female lower genital tract remain largely unknown. With all the obvious restrictions on studying these mechanisms in humans, their understanding depends on the development of adequate experimental models. Isolated cell cultures do not faithfully reproduce important aspects of cell-cell interactions in living tissues and tissue responses to pathogens. Explants and other types of ex vivo tissue models serve as a bridge between cell culture and tissues in vivo. Herein, we discuss various cervico-vaginal tissue models and their use in studying HIV vaginal transmission and consider future directions of such studies.",
keywords = "Cervix, Explants, HIV, Tissue-culture, Transmission",
author = "Melanie Merbah and Andrea Introini and Wendy Fitzgerald and Grivel, {Jean-Charles B.} and Andrea Lisco and Christophe Vanpouille and Leonid Margolis",
year = "2011",
month = "3",
doi = "10.1111/j.1600-0897.2010.00967.x",
language = "English",
volume = "65",
pages = "268--278",
journal = "American Journal of Reproductive Immunology",
issn = "1046-7408",
publisher = "Wiley-Blackwell",
number = "3",

}

TY - JOUR

T1 - Cervico-Vaginal Tissue Ex Vivo as a Model to Study Early Events in HIV-1 Infection

AU - Merbah, Melanie

AU - Introini, Andrea

AU - Fitzgerald, Wendy

AU - Grivel, Jean-Charles B.

AU - Lisco, Andrea

AU - Vanpouille, Christophe

AU - Margolis, Leonid

PY - 2011/3

Y1 - 2011/3

N2 - Citation Merbah M, Introini A, Fitzgerald W, Grivel J-C, Lisco A, Vanpouille C, Margolis L. Cervico-vaginal tissue ex vivo as a model to study early events in HIV-1 infection. Am J Reprod Immunol 2011; 65: 268-278 Vaginal intercourse remains the most prevalent route of infection of women. In spite of many efforts, the detailed mechanisms of HIV-1 transmission in the female lower genital tract remain largely unknown. With all the obvious restrictions on studying these mechanisms in humans, their understanding depends on the development of adequate experimental models. Isolated cell cultures do not faithfully reproduce important aspects of cell-cell interactions in living tissues and tissue responses to pathogens. Explants and other types of ex vivo tissue models serve as a bridge between cell culture and tissues in vivo. Herein, we discuss various cervico-vaginal tissue models and their use in studying HIV vaginal transmission and consider future directions of such studies.

AB - Citation Merbah M, Introini A, Fitzgerald W, Grivel J-C, Lisco A, Vanpouille C, Margolis L. Cervico-vaginal tissue ex vivo as a model to study early events in HIV-1 infection. Am J Reprod Immunol 2011; 65: 268-278 Vaginal intercourse remains the most prevalent route of infection of women. In spite of many efforts, the detailed mechanisms of HIV-1 transmission in the female lower genital tract remain largely unknown. With all the obvious restrictions on studying these mechanisms in humans, their understanding depends on the development of adequate experimental models. Isolated cell cultures do not faithfully reproduce important aspects of cell-cell interactions in living tissues and tissue responses to pathogens. Explants and other types of ex vivo tissue models serve as a bridge between cell culture and tissues in vivo. Herein, we discuss various cervico-vaginal tissue models and their use in studying HIV vaginal transmission and consider future directions of such studies.

KW - Cervix

KW - Explants

KW - HIV

KW - Tissue-culture

KW - Transmission

UR - http://www.scopus.com/inward/record.url?scp=79551648725&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=79551648725&partnerID=8YFLogxK

U2 - 10.1111/j.1600-0897.2010.00967.x

DO - 10.1111/j.1600-0897.2010.00967.x

M3 - Review article

C2 - 21223429

AN - SCOPUS:79551648725

VL - 65

SP - 268

EP - 278

JO - American Journal of Reproductive Immunology

JF - American Journal of Reproductive Immunology

SN - 1046-7408

IS - 3

ER -