Cerebral venous sinus thrombosis

Developing an experimental model

Amit Kumar Srivastava, Rakesh Kumar Gupta, Mohammad Haris, Madhur Ray, Jayantee Kalita, Usha Kant Misra

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

To develop a simple experimental model for cerebral venous sinus thrombosis in rat, 24 male Sprague-Dawley rats (14 experimental and 10 controls) were studied. After anesthesia, a 1.5 mm × 10 mm cranial window was made to expose superior sagittal sinus (SSS). A filter paper strip soaked in 40% ferric chloride topically applied for 5 min to induce SSS thrombosis in experimental group. Five of these rats underwent autopsy at 24 h after rota rod study, other 9 rats underwent MRI after 24 h followed by autopsy at 36-48 h. On MRI, T2 hyperintense lesions were seen in all 9 rats of study group but none in controls. On autopsy, TTC staining revealed changes in 7 out of 14 experimental rats but in none of the controls. The rota rod study results revealed an insignificant difference in the experimental and control group. There was no spontaneous death in experimental or control group during the study. The present experimental model is simple, easy to carry out and results in TTC staining changes in 50% and T2 hyperintensity on MRI in all which could be due to SSS thrombosis.

Original languageEnglish
Pages (from-to)220-222
Number of pages3
JournalJournal of Neuroscience Methods
Volume161
Issue number2
DOIs
Publication statusPublished - 15 Apr 2007
Externally publishedYes

Fingerprint

Intracranial Sinus Thrombosis
Venous Thrombosis
Superior Sagittal Sinus
Theoretical Models
Sagittal Sinus Thrombosis
Autopsy
Staining and Labeling
Control Groups
Sprague Dawley Rats
Anesthesia

Keywords

  • Autopsy
  • MRI
  • Rat model
  • Thrombosis
  • Venous sinus

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Cerebral venous sinus thrombosis : Developing an experimental model. / Srivastava, Amit Kumar; Gupta, Rakesh Kumar; Haris, Mohammad; Ray, Madhur; Kalita, Jayantee; Misra, Usha Kant.

In: Journal of Neuroscience Methods, Vol. 161, No. 2, 15.04.2007, p. 220-222.

Research output: Contribution to journalArticle

Srivastava, Amit Kumar ; Gupta, Rakesh Kumar ; Haris, Mohammad ; Ray, Madhur ; Kalita, Jayantee ; Misra, Usha Kant. / Cerebral venous sinus thrombosis : Developing an experimental model. In: Journal of Neuroscience Methods. 2007 ; Vol. 161, No. 2. pp. 220-222.
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