Cellulose nanocrystal/gold nanoparticle composite as a matrix for enzyme immobilization

Khaled A. Mahmoud, Keith B. Male, Sabahudin Hrapovic, John H.T. Luong

Research output: Contribution to journalArticle

125 Citations (Scopus)


A novel nanocomposite consisting of cellulose nanocrystals (CNCs) functionalized with gold nanoparticles (AuNPs) serving as an excellent support for enzyme immobilization with phenomenally high loading is presented in this work. As testing models, cyclodextrin glycosyl transferase (CGTase) and alcohol oxidase were conjugated on an activated CNC/AuNP matrix. This catalytic platform exhibits significant biocatalytic activity with excellent enzyme stability and without apparent loss of the original activity. The recovered specific activities were ∼70% and 95% for CGTase and alcohol oxidase, respectively. This novel and inexpensive material is anticipated to extend to other enzymes, enhancing the enzyme loading and activity as well as the stability in both operation and storage.

Original languageEnglish
Pages (from-to)1383-1386
Number of pages4
JournalACS Applied Materials and Interfaces
Issue number7
Publication statusPublished - 29 Jul 2009



  • CGTase
  • cellulose nanocrystals
  • enzyme
  • gold nanoparticles
  • immobilization

ASJC Scopus subject areas

  • Materials Science(all)

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