Cell Adhesive and Antifouling Polyvinyl Chloride Surfaces Via Wet Chemical Modification

Matthias Gabriel, Dennis Strand, Christian Friedrich Vahl

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Polyvinyl chloride (PVC) is one of the most frequently used polymers for the manufacturing of medical devices. Limitations for its usage are based upon unfavorable surface properties of the polymer including its hydrophobicity and lack of functionalities in order to increase its versatility. To address this issue, wet chemical modification of PVC was performed through surface amination using the bifunctional compound ethylene diamine. The reaction was conducted in order to achieve maximum surface amination while leaving the bulk material unaffected. The initial activation step was characterized by means of various methods including contact angle measurements, colorimetric amine quantification, infrared spectroscopy, and gel permeation chromatography. Depth profiles were obtained by a confocal microscopic method using fluorescence labeling. Exclusive surface modification was thus confirmed. To demonstrate biological applications of the presented technique, two examples were chosen: The covalent immobilization of the cell adhesive Asp-Gly-Asp-Ser-peptide (RGD) onto PVC samples yielded a surface that strongly supported cellular adhesion and proliferation of fibroblasts. In contrast, the decoration of PVC with the hydrophilic polymer polyethylene glycol prevented cellular adhesion to a large extent. The impact of these modifications was demonstrated by cell culture experiments.

Original languageEnglish
Pages (from-to)839-844
Number of pages6
JournalArtificial Organs
Volume36
Issue number9
DOIs
Publication statusPublished - Sep 2012
Externally publishedYes

Fingerprint

Chemical modification
Polyvinyl Chloride
Polyvinyl chlorides
Amination
Polymers
Adhesion
Diamines
Surface Properties
Gel permeation chromatography
Fibroblasts
Hydrophobicity
Angle measurement
Hydrophobic and Hydrophilic Interactions
Cell culture
Immobilization
Adhesives
Labeling
Peptides
Polyethylene glycols
Contact angle

Keywords

  • Antifouling surface
  • Asp-Gly-Asp-Ser-peptide (RGD) immobilization
  • PEGylation
  • Polyvinylchloride
  • Surface modification

ASJC Scopus subject areas

  • Biomaterials
  • Biomedical Engineering
  • Bioengineering
  • Medicine (miscellaneous)

Cite this

Cell Adhesive and Antifouling Polyvinyl Chloride Surfaces Via Wet Chemical Modification. / Gabriel, Matthias; Strand, Dennis; Vahl, Christian Friedrich.

In: Artificial Organs, Vol. 36, No. 9, 09.2012, p. 839-844.

Research output: Contribution to journalArticle

Gabriel, Matthias ; Strand, Dennis ; Vahl, Christian Friedrich. / Cell Adhesive and Antifouling Polyvinyl Chloride Surfaces Via Wet Chemical Modification. In: Artificial Organs. 2012 ; Vol. 36, No. 9. pp. 839-844.
@article{be1ff8b155c7423f8cdeb302badb4ddb,
title = "Cell Adhesive and Antifouling Polyvinyl Chloride Surfaces Via Wet Chemical Modification",
abstract = "Polyvinyl chloride (PVC) is one of the most frequently used polymers for the manufacturing of medical devices. Limitations for its usage are based upon unfavorable surface properties of the polymer including its hydrophobicity and lack of functionalities in order to increase its versatility. To address this issue, wet chemical modification of PVC was performed through surface amination using the bifunctional compound ethylene diamine. The reaction was conducted in order to achieve maximum surface amination while leaving the bulk material unaffected. The initial activation step was characterized by means of various methods including contact angle measurements, colorimetric amine quantification, infrared spectroscopy, and gel permeation chromatography. Depth profiles were obtained by a confocal microscopic method using fluorescence labeling. Exclusive surface modification was thus confirmed. To demonstrate biological applications of the presented technique, two examples were chosen: The covalent immobilization of the cell adhesive Asp-Gly-Asp-Ser-peptide (RGD) onto PVC samples yielded a surface that strongly supported cellular adhesion and proliferation of fibroblasts. In contrast, the decoration of PVC with the hydrophilic polymer polyethylene glycol prevented cellular adhesion to a large extent. The impact of these modifications was demonstrated by cell culture experiments.",
keywords = "Antifouling surface, Asp-Gly-Asp-Ser-peptide (RGD) immobilization, PEGylation, Polyvinylchloride, Surface modification",
author = "Matthias Gabriel and Dennis Strand and Vahl, {Christian Friedrich}",
year = "2012",
month = "9",
doi = "10.1111/j.1525-1594.2012.01462.x",
language = "English",
volume = "36",
pages = "839--844",
journal = "Artificial Organs",
issn = "0160-564X",
publisher = "Wiley-Blackwell",
number = "9",

}

TY - JOUR

T1 - Cell Adhesive and Antifouling Polyvinyl Chloride Surfaces Via Wet Chemical Modification

AU - Gabriel, Matthias

AU - Strand, Dennis

AU - Vahl, Christian Friedrich

PY - 2012/9

Y1 - 2012/9

N2 - Polyvinyl chloride (PVC) is one of the most frequently used polymers for the manufacturing of medical devices. Limitations for its usage are based upon unfavorable surface properties of the polymer including its hydrophobicity and lack of functionalities in order to increase its versatility. To address this issue, wet chemical modification of PVC was performed through surface amination using the bifunctional compound ethylene diamine. The reaction was conducted in order to achieve maximum surface amination while leaving the bulk material unaffected. The initial activation step was characterized by means of various methods including contact angle measurements, colorimetric amine quantification, infrared spectroscopy, and gel permeation chromatography. Depth profiles were obtained by a confocal microscopic method using fluorescence labeling. Exclusive surface modification was thus confirmed. To demonstrate biological applications of the presented technique, two examples were chosen: The covalent immobilization of the cell adhesive Asp-Gly-Asp-Ser-peptide (RGD) onto PVC samples yielded a surface that strongly supported cellular adhesion and proliferation of fibroblasts. In contrast, the decoration of PVC with the hydrophilic polymer polyethylene glycol prevented cellular adhesion to a large extent. The impact of these modifications was demonstrated by cell culture experiments.

AB - Polyvinyl chloride (PVC) is one of the most frequently used polymers for the manufacturing of medical devices. Limitations for its usage are based upon unfavorable surface properties of the polymer including its hydrophobicity and lack of functionalities in order to increase its versatility. To address this issue, wet chemical modification of PVC was performed through surface amination using the bifunctional compound ethylene diamine. The reaction was conducted in order to achieve maximum surface amination while leaving the bulk material unaffected. The initial activation step was characterized by means of various methods including contact angle measurements, colorimetric amine quantification, infrared spectroscopy, and gel permeation chromatography. Depth profiles were obtained by a confocal microscopic method using fluorescence labeling. Exclusive surface modification was thus confirmed. To demonstrate biological applications of the presented technique, two examples were chosen: The covalent immobilization of the cell adhesive Asp-Gly-Asp-Ser-peptide (RGD) onto PVC samples yielded a surface that strongly supported cellular adhesion and proliferation of fibroblasts. In contrast, the decoration of PVC with the hydrophilic polymer polyethylene glycol prevented cellular adhesion to a large extent. The impact of these modifications was demonstrated by cell culture experiments.

KW - Antifouling surface

KW - Asp-Gly-Asp-Ser-peptide (RGD) immobilization

KW - PEGylation

KW - Polyvinylchloride

KW - Surface modification

UR - http://www.scopus.com/inward/record.url?scp=84866156851&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84866156851&partnerID=8YFLogxK

U2 - 10.1111/j.1525-1594.2012.01462.x

DO - 10.1111/j.1525-1594.2012.01462.x

M3 - Article

VL - 36

SP - 839

EP - 844

JO - Artificial Organs

JF - Artificial Organs

SN - 0160-564X

IS - 9

ER -