CD2 distinguishes two subsets of human plasmacytoid dendritic cells with distinct phenotype and functions

Toshimichi Matsui, John E. Connolly, Mark Michnevitz, Damien Chaussabel, Chun I. Yu, Casey Glaser, Sasha Tindle, Marc Pypaert, Heidi Freitas, Bernard Piqueras, Jacques Banchereau, A. Karolina Palucka

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Abstract

Plasmacytoid dendritic cells (pDCs) are key regulators of antiviral immunity. They rapidly secrete IFN-α and cross-present viral Ags, thereby launching adaptive immunity. In this study, we show that activated human pDCs inhibit replication of cancer cells and kill them in a contact-dependent fashion. Expression of CD2 distinguishes two pDC subsets with distinct phenotype and function. Both subsets secrete IFN-α and express granzyme B and TRAIL. CD2high pDCs uniquely express lysozyme and can be found in tonsils and in tumors. Both subsets launch recall T cell responses. However, CD2high pDCs secrete higher levels of IL12p40, express higher levels of costimulatory molecule CD80, and are more efficient in triggering proliferation of naive allogeneic T cells. Thus, human blood pDCs are composed of subsets with specific phenotype and functions.

Original languageEnglish
Pages (from-to)6815-6823
Number of pages9
JournalJournal of Immunology
Volume182
Issue number11
DOIs
Publication statusPublished - 1 Jun 2009

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ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

Cite this

Matsui, T., Connolly, J. E., Michnevitz, M., Chaussabel, D., Yu, C. I., Glaser, C., Tindle, S., Pypaert, M., Freitas, H., Piqueras, B., Banchereau, J., & Karolina Palucka, A. (2009). CD2 distinguishes two subsets of human plasmacytoid dendritic cells with distinct phenotype and functions. Journal of Immunology, 182(11), 6815-6823. https://doi.org/10.4049/jimmunol.0802008