CD2 distinguishes two subsets of human plasmacytoid dendritic cells with distinct phenotype and functions

Toshimichi Matsui, John E. Connolly, Mark Michnevitz, Damien J. Chaussabel, Chun I. Yu, Casey Glaser, Sasha Tindle, Marc Pypaert, Heidi Freitas, Bernard Piqueras, Jacques Banchereau, A. Karolina Palucka

Research output: Contribution to journalArticle

101 Citations (Scopus)

Abstract

Plasmacytoid dendritic cells (pDCs) are key regulators of antiviral immunity. They rapidly secrete IFN-α and cross-present viral Ags, thereby launching adaptive immunity. In this study, we show that activated human pDCs inhibit replication of cancer cells and kill them in a contact-dependent fashion. Expression of CD2 distinguishes two pDC subsets with distinct phenotype and function. Both subsets secrete IFN-α and express granzyme B and TRAIL. CD2 high pDCs uniquely express lysozyme and can be found in tonsils and in tumors. Both subsets launch recall T cell responses. However, CD2 high pDCs secrete higher levels of IL12p40, express higher levels of costimulatory molecule CD80, and are more efficient in triggering proliferation of naive allogeneic T cells. Thus, human blood pDCs are composed of subsets with specific phenotype and functions.

Original languageEnglish
Pages (from-to)6815-6823
Number of pages9
JournalJournal of Immunology
Volume182
Issue number11
DOIs
Publication statusPublished - 1 Jun 2009
Externally publishedYes

Fingerprint

Dendritic Cells
Phenotype
T-Lymphocytes
Granzymes
Palatine Tonsil
Adaptive Immunity
Muramidase
Antiviral Agents
Immunity
Neoplasms

ASJC Scopus subject areas

  • Immunology

Cite this

Matsui, T., Connolly, J. E., Michnevitz, M., Chaussabel, D. J., Yu, C. I., Glaser, C., ... Karolina Palucka, A. (2009). CD2 distinguishes two subsets of human plasmacytoid dendritic cells with distinct phenotype and functions. Journal of Immunology, 182(11), 6815-6823. https://doi.org/10.4049/jimmunol.0802008

CD2 distinguishes two subsets of human plasmacytoid dendritic cells with distinct phenotype and functions. / Matsui, Toshimichi; Connolly, John E.; Michnevitz, Mark; Chaussabel, Damien J.; Yu, Chun I.; Glaser, Casey; Tindle, Sasha; Pypaert, Marc; Freitas, Heidi; Piqueras, Bernard; Banchereau, Jacques; Karolina Palucka, A.

In: Journal of Immunology, Vol. 182, No. 11, 01.06.2009, p. 6815-6823.

Research output: Contribution to journalArticle

Matsui, T, Connolly, JE, Michnevitz, M, Chaussabel, DJ, Yu, CI, Glaser, C, Tindle, S, Pypaert, M, Freitas, H, Piqueras, B, Banchereau, J & Karolina Palucka, A 2009, 'CD2 distinguishes two subsets of human plasmacytoid dendritic cells with distinct phenotype and functions', Journal of Immunology, vol. 182, no. 11, pp. 6815-6823. https://doi.org/10.4049/jimmunol.0802008
Matsui, Toshimichi ; Connolly, John E. ; Michnevitz, Mark ; Chaussabel, Damien J. ; Yu, Chun I. ; Glaser, Casey ; Tindle, Sasha ; Pypaert, Marc ; Freitas, Heidi ; Piqueras, Bernard ; Banchereau, Jacques ; Karolina Palucka, A. / CD2 distinguishes two subsets of human plasmacytoid dendritic cells with distinct phenotype and functions. In: Journal of Immunology. 2009 ; Vol. 182, No. 11. pp. 6815-6823.
@article{99ce0865e904435c9dfeb1a3d4a1b89e,
title = "CD2 distinguishes two subsets of human plasmacytoid dendritic cells with distinct phenotype and functions",
abstract = "Plasmacytoid dendritic cells (pDCs) are key regulators of antiviral immunity. They rapidly secrete IFN-α and cross-present viral Ags, thereby launching adaptive immunity. In this study, we show that activated human pDCs inhibit replication of cancer cells and kill them in a contact-dependent fashion. Expression of CD2 distinguishes two pDC subsets with distinct phenotype and function. Both subsets secrete IFN-α and express granzyme B and TRAIL. CD2 high pDCs uniquely express lysozyme and can be found in tonsils and in tumors. Both subsets launch recall T cell responses. However, CD2 high pDCs secrete higher levels of IL12p40, express higher levels of costimulatory molecule CD80, and are more efficient in triggering proliferation of naive allogeneic T cells. Thus, human blood pDCs are composed of subsets with specific phenotype and functions.",
author = "Toshimichi Matsui and Connolly, {John E.} and Mark Michnevitz and Chaussabel, {Damien J.} and Yu, {Chun I.} and Casey Glaser and Sasha Tindle and Marc Pypaert and Heidi Freitas and Bernard Piqueras and Jacques Banchereau and {Karolina Palucka}, A.",
year = "2009",
month = "6",
day = "1",
doi = "10.4049/jimmunol.0802008",
language = "English",
volume = "182",
pages = "6815--6823",
journal = "Journal of Immunology",
issn = "0022-1767",
publisher = "American Association of Immunologists",
number = "11",

}

TY - JOUR

T1 - CD2 distinguishes two subsets of human plasmacytoid dendritic cells with distinct phenotype and functions

AU - Matsui, Toshimichi

AU - Connolly, John E.

AU - Michnevitz, Mark

AU - Chaussabel, Damien J.

AU - Yu, Chun I.

AU - Glaser, Casey

AU - Tindle, Sasha

AU - Pypaert, Marc

AU - Freitas, Heidi

AU - Piqueras, Bernard

AU - Banchereau, Jacques

AU - Karolina Palucka, A.

PY - 2009/6/1

Y1 - 2009/6/1

N2 - Plasmacytoid dendritic cells (pDCs) are key regulators of antiviral immunity. They rapidly secrete IFN-α and cross-present viral Ags, thereby launching adaptive immunity. In this study, we show that activated human pDCs inhibit replication of cancer cells and kill them in a contact-dependent fashion. Expression of CD2 distinguishes two pDC subsets with distinct phenotype and function. Both subsets secrete IFN-α and express granzyme B and TRAIL. CD2 high pDCs uniquely express lysozyme and can be found in tonsils and in tumors. Both subsets launch recall T cell responses. However, CD2 high pDCs secrete higher levels of IL12p40, express higher levels of costimulatory molecule CD80, and are more efficient in triggering proliferation of naive allogeneic T cells. Thus, human blood pDCs are composed of subsets with specific phenotype and functions.

AB - Plasmacytoid dendritic cells (pDCs) are key regulators of antiviral immunity. They rapidly secrete IFN-α and cross-present viral Ags, thereby launching adaptive immunity. In this study, we show that activated human pDCs inhibit replication of cancer cells and kill them in a contact-dependent fashion. Expression of CD2 distinguishes two pDC subsets with distinct phenotype and function. Both subsets secrete IFN-α and express granzyme B and TRAIL. CD2 high pDCs uniquely express lysozyme and can be found in tonsils and in tumors. Both subsets launch recall T cell responses. However, CD2 high pDCs secrete higher levels of IL12p40, express higher levels of costimulatory molecule CD80, and are more efficient in triggering proliferation of naive allogeneic T cells. Thus, human blood pDCs are composed of subsets with specific phenotype and functions.

UR - http://www.scopus.com/inward/record.url?scp=67449128181&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=67449128181&partnerID=8YFLogxK

U2 - 10.4049/jimmunol.0802008

DO - 10.4049/jimmunol.0802008

M3 - Article

VL - 182

SP - 6815

EP - 6823

JO - Journal of Immunology

JF - Journal of Immunology

SN - 0022-1767

IS - 11

ER -