CD103 mRNA levels in urinary cells predict acute rejection of renal allografts

Ruchuang Ding, Baogui Li, Thangamani Muthukumar, Darshana Dadhania, Mara Medeiros, Choli Hartono, David Serur, Surya V. Seshan, Vijay K. Sharma, Sandip Kapur, Manikkam Suthanthiran

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Abstract

Background. CD103 is displayed on the cell surface of alloreactive CD8 cytotoxic T lymphocytes (CTLs) and is a critical component for the intraepithelial homing of T cells. Because intratubular localization of mononuclear cells is a feature of acute cellular rejection of renal allografts, we explored the hypothesis that CD103 messenger (m)RNA levels in urinary cells predict acute rejection. Methods. We collected 89 urine specimens from 79 recipients of renal allografts. RNA was isolated from the urinary cells, and we measured CD103 mRNA levels and a constitutively expressed 18S ribosomal (r)RNA with the use of real-time quantitative polymerase chain reaction assay. Results. CD103 mRNA levels, but not 18S rRNA levels, were higher in urinary cells from 30 patients with an episode of acute rejection (32 biopsies and 32 urine samples) compared with the levels in 12 patients with other findings on allograft biopsy (12 biopsies and 12 urine samples), 12 patients with biopsy evidence of chronic allograft nephropathy (12 biopsies and 12 urine samples), and 25 patients with stable graft function after renal transplantation (0 biopsies and 33 urine samples) (P = 0.001; one-way analysis of variance). Acute rejection was predicted with a sensitivity of 59% and a specificity of 75% using natural log-transformed value 8.16 CD103 copies per microgram as the cutoff value (P = 0.001). Conclusion. CD103 mRNA levels in urinary cells are diagnostic of acute rejection of renal allografts. Because CD103 is a cell surface marker of intratubular CD8 CTLs, a noninvasive assessment of cellular traffic into the allograft may be feasible by the measurement of CD103 mRNA levels in urinary cells.

Original languageEnglish
Pages (from-to)1307-1312
Number of pages6
JournalTransplantation
Volume75
Issue number8
DOIs
Publication statusPublished - 27 Apr 2003
Externally publishedYes

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Allografts
Kidney
Messenger RNA
Biopsy
Urine
Cytotoxic T-Lymphocytes
18S Ribosomal RNA
Kidney Transplantation
Real-Time Polymerase Chain Reaction
Analysis of Variance
RNA
T-Lymphocytes
Transplants

ASJC Scopus subject areas

  • Transplantation

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CD103 mRNA levels in urinary cells predict acute rejection of renal allografts. / Ding, Ruchuang; Li, Baogui; Muthukumar, Thangamani; Dadhania, Darshana; Medeiros, Mara; Hartono, Choli; Serur, David; Seshan, Surya V.; Sharma, Vijay K.; Kapur, Sandip; Suthanthiran, Manikkam.

In: Transplantation, Vol. 75, No. 8, 27.04.2003, p. 1307-1312.

Research output: Contribution to journalArticle

Ding, R, Li, B, Muthukumar, T, Dadhania, D, Medeiros, M, Hartono, C, Serur, D, Seshan, SV, Sharma, VK, Kapur, S & Suthanthiran, M 2003, 'CD103 mRNA levels in urinary cells predict acute rejection of renal allografts', Transplantation, vol. 75, no. 8, pp. 1307-1312. https://doi.org/10.1097/01.TP.0000064210.92444.B5
Ding R, Li B, Muthukumar T, Dadhania D, Medeiros M, Hartono C et al. CD103 mRNA levels in urinary cells predict acute rejection of renal allografts. Transplantation. 2003 Apr 27;75(8):1307-1312. https://doi.org/10.1097/01.TP.0000064210.92444.B5
Ding, Ruchuang ; Li, Baogui ; Muthukumar, Thangamani ; Dadhania, Darshana ; Medeiros, Mara ; Hartono, Choli ; Serur, David ; Seshan, Surya V. ; Sharma, Vijay K. ; Kapur, Sandip ; Suthanthiran, Manikkam. / CD103 mRNA levels in urinary cells predict acute rejection of renal allografts. In: Transplantation. 2003 ; Vol. 75, No. 8. pp. 1307-1312.
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T1 - CD103 mRNA levels in urinary cells predict acute rejection of renal allografts

AU - Ding, Ruchuang

AU - Li, Baogui

AU - Muthukumar, Thangamani

AU - Dadhania, Darshana

AU - Medeiros, Mara

AU - Hartono, Choli

AU - Serur, David

AU - Seshan, Surya V.

AU - Sharma, Vijay K.

AU - Kapur, Sandip

AU - Suthanthiran, Manikkam

PY - 2003/4/27

Y1 - 2003/4/27

N2 - Background. CD103 is displayed on the cell surface of alloreactive CD8 cytotoxic T lymphocytes (CTLs) and is a critical component for the intraepithelial homing of T cells. Because intratubular localization of mononuclear cells is a feature of acute cellular rejection of renal allografts, we explored the hypothesis that CD103 messenger (m)RNA levels in urinary cells predict acute rejection. Methods. We collected 89 urine specimens from 79 recipients of renal allografts. RNA was isolated from the urinary cells, and we measured CD103 mRNA levels and a constitutively expressed 18S ribosomal (r)RNA with the use of real-time quantitative polymerase chain reaction assay. Results. CD103 mRNA levels, but not 18S rRNA levels, were higher in urinary cells from 30 patients with an episode of acute rejection (32 biopsies and 32 urine samples) compared with the levels in 12 patients with other findings on allograft biopsy (12 biopsies and 12 urine samples), 12 patients with biopsy evidence of chronic allograft nephropathy (12 biopsies and 12 urine samples), and 25 patients with stable graft function after renal transplantation (0 biopsies and 33 urine samples) (P = 0.001; one-way analysis of variance). Acute rejection was predicted with a sensitivity of 59% and a specificity of 75% using natural log-transformed value 8.16 CD103 copies per microgram as the cutoff value (P = 0.001). Conclusion. CD103 mRNA levels in urinary cells are diagnostic of acute rejection of renal allografts. Because CD103 is a cell surface marker of intratubular CD8 CTLs, a noninvasive assessment of cellular traffic into the allograft may be feasible by the measurement of CD103 mRNA levels in urinary cells.

AB - Background. CD103 is displayed on the cell surface of alloreactive CD8 cytotoxic T lymphocytes (CTLs) and is a critical component for the intraepithelial homing of T cells. Because intratubular localization of mononuclear cells is a feature of acute cellular rejection of renal allografts, we explored the hypothesis that CD103 messenger (m)RNA levels in urinary cells predict acute rejection. Methods. We collected 89 urine specimens from 79 recipients of renal allografts. RNA was isolated from the urinary cells, and we measured CD103 mRNA levels and a constitutively expressed 18S ribosomal (r)RNA with the use of real-time quantitative polymerase chain reaction assay. Results. CD103 mRNA levels, but not 18S rRNA levels, were higher in urinary cells from 30 patients with an episode of acute rejection (32 biopsies and 32 urine samples) compared with the levels in 12 patients with other findings on allograft biopsy (12 biopsies and 12 urine samples), 12 patients with biopsy evidence of chronic allograft nephropathy (12 biopsies and 12 urine samples), and 25 patients with stable graft function after renal transplantation (0 biopsies and 33 urine samples) (P = 0.001; one-way analysis of variance). Acute rejection was predicted with a sensitivity of 59% and a specificity of 75% using natural log-transformed value 8.16 CD103 copies per microgram as the cutoff value (P = 0.001). Conclusion. CD103 mRNA levels in urinary cells are diagnostic of acute rejection of renal allografts. Because CD103 is a cell surface marker of intratubular CD8 CTLs, a noninvasive assessment of cellular traffic into the allograft may be feasible by the measurement of CD103 mRNA levels in urinary cells.

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