Ca2+ homeostasis regulates Xenopus oocyte maturation

Lu Sun, Rawad Hodeify, Shirley Haun, Amanda Charlesworth, Angus M. MacNicol, Subramaniam Ponnappan, Usha Ponnappan, Claude Prigent, Khaled Machaca

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

In contrast to the well-defined role of Ca2+ signals during mitosis, the contribution of Ca2+ signaling to meiosis progression is controversial, despite several decades of investigating the role of Ca 2+ and its effectors in vertebrate oocyte maturation. We have previously shown that during Xenopus oocyte maturation, Ca2+ signals are dispensable for entry into meiosis and for germinal vesicle breakdown. However, normal Ca2+ homeostasis is essential for completion of meiosis I and extrusion of the first polar body. In this study, we test the contribution of several downstream effectors in mediating the Ca2+ effects during oocyte maturation. We show that calmodulin and calcium-calmodulin-dependent protein kinase II (CAMK2) are not critical downstream Ca2+ effectors during meiotic maturation. In contrast, accumulation of Aurora kinase A (AURKA) protein is disrupted in cells deprived of Ca2+ signals. Since AURKA is required for bipolar spindle formation, failure to accumulate AURKA may contribute to the defective spindle phenotype following Ca2+ deprivation. These findings argue that Ca2+ homeostasis is important in establishing the oocyte's competence to undergo maturation in preparation for fertilization and embryonic development.

Original languageEnglish
Pages (from-to)726-735
Number of pages10
JournalBiology of Reproduction
Volume78
Issue number4
DOIs
Publication statusPublished - Apr 2008
Externally publishedYes

Fingerprint

Aurora Kinase A
Xenopus
Oocytes
Meiosis
Homeostasis
Calcium-Calmodulin-Dependent Protein Kinase Type 2
Polar Bodies
Calcium-Calmodulin-Dependent Protein Kinases
Calmodulin
Mitosis
Fertilization
Mental Competency
Embryonic Development
Vertebrates
Phenotype
Proteins

Keywords

  • Aurora kinase A
  • Calcium
  • Gamete biology
  • Meiosis
  • Oocyte development

ASJC Scopus subject areas

  • Cell Biology
  • Developmental Biology
  • Embryology

Cite this

Ca2+ homeostasis regulates Xenopus oocyte maturation. / Sun, Lu; Hodeify, Rawad; Haun, Shirley; Charlesworth, Amanda; MacNicol, Angus M.; Ponnappan, Subramaniam; Ponnappan, Usha; Prigent, Claude; Machaca, Khaled.

In: Biology of Reproduction, Vol. 78, No. 4, 04.2008, p. 726-735.

Research output: Contribution to journalArticle

Sun, L, Hodeify, R, Haun, S, Charlesworth, A, MacNicol, AM, Ponnappan, S, Ponnappan, U, Prigent, C & Machaca, K 2008, 'Ca2+ homeostasis regulates Xenopus oocyte maturation', Biology of Reproduction, vol. 78, no. 4, pp. 726-735. https://doi.org/10.1095/biolreprod.107.063693
Sun, Lu ; Hodeify, Rawad ; Haun, Shirley ; Charlesworth, Amanda ; MacNicol, Angus M. ; Ponnappan, Subramaniam ; Ponnappan, Usha ; Prigent, Claude ; Machaca, Khaled. / Ca2+ homeostasis regulates Xenopus oocyte maturation. In: Biology of Reproduction. 2008 ; Vol. 78, No. 4. pp. 726-735.
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