Caspase-mediated cleavage of HuR in the cytoplasm contributes to pp32/PHAP-I regulation of apoptosis

Rachid Mazroui, Sergio Di Marco, Eveline Clair, Christopher Von Roretz, Scott A. Tenenbaum, Jack D. Keene, Maya Saleh, Imed Eddine Gallouzi

Research output: Contribution to journalArticle

82 Citations (Scopus)

Abstract

The RNA-binding protein HuR affects cell fate by regulating the stability and/or the translation of messenger RNAs that encode cell stress response proteins. In this study, we delineate a novel regulatory mechanism by which HuR contributes to stress-induced cell death. Upon lethal stress, HuR translocates into the cytoplasm by a mechanism involving its association with the apoptosome activator pp32/PHAP-I. Depleting the expression of pp32/PHAP-I by RNA interference reduces both HuR cytoplasmic accumulation and the efficiency of caspase activation. In the cytoplasm, HuR undergoes caspase-mediated cleavage at aspartate 226. This cleavage activity is significantly reduced in the absence of pp32/PHAP-I. Substituting aspartate 226 with an alanine creates a noncleavable isoform of HuR that, when over expressed, maintains its association with pp32/PHAP-I and delays the apoptotic response. Thus, we propose a model in which HuR association with pp32/PHAP-I and its caspase-mediated cleavage constitutes a regulatory step that contributes to an amplified apoptotic response.

Original languageEnglish
Pages (from-to)113-127
Number of pages15
JournalJournal of Cell Biology
Volume180
Issue number1
DOIs
Publication statusPublished - 14 Jan 2008

    Fingerprint

ASJC Scopus subject areas

  • Cell Biology

Cite this

Mazroui, R., Di Marco, S., Clair, E., Von Roretz, C., Tenenbaum, S. A., Keene, J. D., Saleh, M., & Gallouzi, I. E. (2008). Caspase-mediated cleavage of HuR in the cytoplasm contributes to pp32/PHAP-I regulation of apoptosis. Journal of Cell Biology, 180(1), 113-127. https://doi.org/10.1083/jcb.200709030