CASP8 -652 6N del and CASP8 IVS12-19G>A gene polymorphisms and susceptibility/prognosis of ESCC

A case control study in northern Indian population

Meenakshi Umar, Rohit Upadhyay, Shaleen Kumar, Uday Chand Ghoshal, Balraj Mittal

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Background Caspase8 influences carcinogenesis through regulation of apoptosis, hyperproliferation, and metastasis. Role of genetic variations in caspase8 has been explored in various cancers; however, their predictive and prognostic role in esophageal cancer is poorly understood. Methods We investigated the association of two potential caspase8 polymorphisms: CASP8 -652 6N del and CASP8 IVS12-19 G>A polymorphisms with susceptibility and survival of 259 esophageal squamous cell carcinoma (ESCC) cases and 259 cancer-free controls from northern Indian population using PCR/PCR RFLP method. Results CASP8 IVS12-19 AA genotype was found to be associated with significant increased risk of ESCC (odds ratio (OR) 3.28, 95% confidence interval (CI) 1.04-10.29) specifically in male subjects (OR 3.71, 95% CI 1.01-13.35) with lower third tumor anatomical location (OR 6.00, 95% CI 1.60-22.55). Kaplan-Meier and Cox Regression analysis showed lower median survival (7.13 months vs. 25.21 months) and greater hazard of death (HR 3.40, 95% CI 1.38-7.90) with CASP8 IVS12-19 AA genotype in ESCC cases compared to IVS12-19 GG genotype. However, no association of CASP8 -652 6N del polymorphism with susceptibility and prognosis of ESCC was observed. Conclusion CASP8 IVS12-19 G>A but not CASP8 -652 6N del polymorphism may modulate risk of ESCC and its survival outcome in northern Indian population.

Original languageEnglish
Pages (from-to)716-723
Number of pages8
JournalJournal of Surgical Oncology
Volume103
Issue number7
DOIs
Publication statusPublished - 1 Jun 2011
Externally publishedYes

Fingerprint

Case-Control Studies
Confidence Intervals
Population
Genes
Odds Ratio
Genotype
Neoplasms
Polymerase Chain Reaction
Esophageal Neoplasms
Restriction Fragment Length Polymorphisms
Carcinogenesis
Regression Analysis
Esophageal Squamous Cell Carcinoma
Apoptosis
Neoplasm Metastasis

Keywords

  • Caspase8
  • ESCC
  • esophageal cancer
  • polymorphism
  • prognosis

ASJC Scopus subject areas

  • Surgery
  • Oncology

Cite this

CASP8 -652 6N del and CASP8 IVS12-19G>A gene polymorphisms and susceptibility/prognosis of ESCC : A case control study in northern Indian population. / Umar, Meenakshi; Upadhyay, Rohit; Kumar, Shaleen; Ghoshal, Uday Chand; Mittal, Balraj.

In: Journal of Surgical Oncology, Vol. 103, No. 7, 01.06.2011, p. 716-723.

Research output: Contribution to journalArticle

@article{c1a6c38450b24aed9fd9c0bea9232f40,
title = "CASP8 -652 6N del and CASP8 IVS12-19G>A gene polymorphisms and susceptibility/prognosis of ESCC: A case control study in northern Indian population",
abstract = "Background Caspase8 influences carcinogenesis through regulation of apoptosis, hyperproliferation, and metastasis. Role of genetic variations in caspase8 has been explored in various cancers; however, their predictive and prognostic role in esophageal cancer is poorly understood. Methods We investigated the association of two potential caspase8 polymorphisms: CASP8 -652 6N del and CASP8 IVS12-19 G>A polymorphisms with susceptibility and survival of 259 esophageal squamous cell carcinoma (ESCC) cases and 259 cancer-free controls from northern Indian population using PCR/PCR RFLP method. Results CASP8 IVS12-19 AA genotype was found to be associated with significant increased risk of ESCC (odds ratio (OR) 3.28, 95{\%} confidence interval (CI) 1.04-10.29) specifically in male subjects (OR 3.71, 95{\%} CI 1.01-13.35) with lower third tumor anatomical location (OR 6.00, 95{\%} CI 1.60-22.55). Kaplan-Meier and Cox Regression analysis showed lower median survival (7.13 months vs. 25.21 months) and greater hazard of death (HR 3.40, 95{\%} CI 1.38-7.90) with CASP8 IVS12-19 AA genotype in ESCC cases compared to IVS12-19 GG genotype. However, no association of CASP8 -652 6N del polymorphism with susceptibility and prognosis of ESCC was observed. Conclusion CASP8 IVS12-19 G>A but not CASP8 -652 6N del polymorphism may modulate risk of ESCC and its survival outcome in northern Indian population.",
keywords = "Caspase8, ESCC, esophageal cancer, polymorphism, prognosis",
author = "Meenakshi Umar and Rohit Upadhyay and Shaleen Kumar and Ghoshal, {Uday Chand} and Balraj Mittal",
year = "2011",
month = "6",
day = "1",
doi = "10.1002/jso.21881",
language = "English",
volume = "103",
pages = "716--723",
journal = "Journal of Surgical Oncology",
issn = "0022-4790",
publisher = "Wiley-Liss Inc.",
number = "7",

}

TY - JOUR

T1 - CASP8 -652 6N del and CASP8 IVS12-19G>A gene polymorphisms and susceptibility/prognosis of ESCC

T2 - A case control study in northern Indian population

AU - Umar, Meenakshi

AU - Upadhyay, Rohit

AU - Kumar, Shaleen

AU - Ghoshal, Uday Chand

AU - Mittal, Balraj

PY - 2011/6/1

Y1 - 2011/6/1

N2 - Background Caspase8 influences carcinogenesis through regulation of apoptosis, hyperproliferation, and metastasis. Role of genetic variations in caspase8 has been explored in various cancers; however, their predictive and prognostic role in esophageal cancer is poorly understood. Methods We investigated the association of two potential caspase8 polymorphisms: CASP8 -652 6N del and CASP8 IVS12-19 G>A polymorphisms with susceptibility and survival of 259 esophageal squamous cell carcinoma (ESCC) cases and 259 cancer-free controls from northern Indian population using PCR/PCR RFLP method. Results CASP8 IVS12-19 AA genotype was found to be associated with significant increased risk of ESCC (odds ratio (OR) 3.28, 95% confidence interval (CI) 1.04-10.29) specifically in male subjects (OR 3.71, 95% CI 1.01-13.35) with lower third tumor anatomical location (OR 6.00, 95% CI 1.60-22.55). Kaplan-Meier and Cox Regression analysis showed lower median survival (7.13 months vs. 25.21 months) and greater hazard of death (HR 3.40, 95% CI 1.38-7.90) with CASP8 IVS12-19 AA genotype in ESCC cases compared to IVS12-19 GG genotype. However, no association of CASP8 -652 6N del polymorphism with susceptibility and prognosis of ESCC was observed. Conclusion CASP8 IVS12-19 G>A but not CASP8 -652 6N del polymorphism may modulate risk of ESCC and its survival outcome in northern Indian population.

AB - Background Caspase8 influences carcinogenesis through regulation of apoptosis, hyperproliferation, and metastasis. Role of genetic variations in caspase8 has been explored in various cancers; however, their predictive and prognostic role in esophageal cancer is poorly understood. Methods We investigated the association of two potential caspase8 polymorphisms: CASP8 -652 6N del and CASP8 IVS12-19 G>A polymorphisms with susceptibility and survival of 259 esophageal squamous cell carcinoma (ESCC) cases and 259 cancer-free controls from northern Indian population using PCR/PCR RFLP method. Results CASP8 IVS12-19 AA genotype was found to be associated with significant increased risk of ESCC (odds ratio (OR) 3.28, 95% confidence interval (CI) 1.04-10.29) specifically in male subjects (OR 3.71, 95% CI 1.01-13.35) with lower third tumor anatomical location (OR 6.00, 95% CI 1.60-22.55). Kaplan-Meier and Cox Regression analysis showed lower median survival (7.13 months vs. 25.21 months) and greater hazard of death (HR 3.40, 95% CI 1.38-7.90) with CASP8 IVS12-19 AA genotype in ESCC cases compared to IVS12-19 GG genotype. However, no association of CASP8 -652 6N del polymorphism with susceptibility and prognosis of ESCC was observed. Conclusion CASP8 IVS12-19 G>A but not CASP8 -652 6N del polymorphism may modulate risk of ESCC and its survival outcome in northern Indian population.

KW - Caspase8

KW - ESCC

KW - esophageal cancer

KW - polymorphism

KW - prognosis

UR - http://www.scopus.com/inward/record.url?scp=79955474495&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=79955474495&partnerID=8YFLogxK

U2 - 10.1002/jso.21881

DO - 10.1002/jso.21881

M3 - Article

VL - 103

SP - 716

EP - 723

JO - Journal of Surgical Oncology

JF - Journal of Surgical Oncology

SN - 0022-4790

IS - 7

ER -