Calcium-dependent regulation of membrane ion permeability in a cell line derived from the equine sweat gland epithelium

Stuart M. Wilson, Wing Hung Ko, John D. Pediani, Soma Rakhit, James A. Nichol, Douglas Bovell

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

We measured the rates of 125I- and 86Rb+ efflux from preloaded, cultured equine sweat gland cells. The calcium ionophore ionomycin increased the efflux of both isotopes. Anion efflux was unaffected by Ba2+, but this cation inhibited 86Rb+-efflux, suggesting that [Ca2+]i-activated potassium channels were present. Activation of these channels was not, however, important for the efflux of anions. We measured 125I- efflux from valinomycin-depolarised cells in which anion cotransport was inhibited. Changes in 125I- efflux reflect changes in anion permeability under these conditions, and ionomycin caused a clear permeability increase that was abolished by the anion channel blocker diphenylamine-2-carboxylate. ATP and UTP increased the efflux of both isotopes, suggesting that type P2U purine receptors allow these nucleotides to regulate membrane permeability.

Original languageEnglish
Pages (from-to)215-221
Number of pages7
JournalComparative Biochemistry and Physiology -- Part A: Physiology
Volume111
Issue number2
DOIs
Publication statusPublished - 1995
Externally publishedYes

Fingerprint

Sweat Glands
Horses
Anions
Permeability
Epithelium
Ions
Calcium
Cell Line
Membranes
Ionomycin
Isotopes
Purinergic P2Y2 Receptors
Valinomycin
Purinergic Receptors
Uridine Triphosphate
Calcium Ionophores
Potassium Channels
Cations
Nucleotides
Adenosine Triphosphate

Keywords

  • I-efflux
  • Rb-efflux
  • Cell culture
  • Intracellular Ca
  • Membrane permeability
  • Purinergic receptors
  • Stimulus-secretion coupling
  • Sweat gland

ASJC Scopus subject areas

  • Physiology

Cite this

Calcium-dependent regulation of membrane ion permeability in a cell line derived from the equine sweat gland epithelium. / Wilson, Stuart M.; Ko, Wing Hung; Pediani, John D.; Rakhit, Soma; Nichol, James A.; Bovell, Douglas.

In: Comparative Biochemistry and Physiology -- Part A: Physiology, Vol. 111, No. 2, 1995, p. 215-221.

Research output: Contribution to journalArticle

Wilson, Stuart M. ; Ko, Wing Hung ; Pediani, John D. ; Rakhit, Soma ; Nichol, James A. ; Bovell, Douglas. / Calcium-dependent regulation of membrane ion permeability in a cell line derived from the equine sweat gland epithelium. In: Comparative Biochemistry and Physiology -- Part A: Physiology. 1995 ; Vol. 111, No. 2. pp. 215-221.
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