Bovine lactoferrin and lactoferricin interfere with intracellular trafficking of Herpes simplex virus-1

Alexandra K. Marr, H. Jenssen, M. Roshan Moniri, R. E W Hancock, N. Panté

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

Although both lactoferrin (Lf), a component of the innate immune system of living organisms, and its N-terminal pepsin cleavage product lactoferricin (Lfcin) have anti-herpes activity, the precise mechanisms by which Lf and Lfcin bring about inhibition of herpes infections are not fully understood. In the present study, experiments were carried out to characterize the activity of bovine Lf and Lfcin (BLf and BLfcin) against the Herpes simplex virus-1 (HSV-1). HSV-1 cellular uptake and intracellular trafficking were studied by immunofluorescence microscopy. In comparison to the untreated infected control cells, both the BLf- and BLfcin-treated cells showed a significant reduction in HSV-1 cellular uptake. The few virus particles that were internalized appeared to have a delayed intracellular trafficking. Thus, in addition to their interference with the uptake of the virus into host cells, Lf and Lfcin also exert their antiviral effect intracellularly.

Original languageEnglish
Pages (from-to)160-164
Number of pages5
JournalBiochimie
Volume91
Issue number1
DOIs
Publication statusPublished - Jan 2009
Externally publishedYes

Fingerprint

Lactoferrin
Human Herpesvirus 1
Viruses
Pepsin A
Fluorescence Microscopy
Virion
Immune system
Antiviral Agents
Immune System
Microscopic examination
Cells
lactoferricin B
Infection
Experiments

Keywords

  • Antiviral
  • Herpes virus
  • HSV-1
  • Lactoferricin
  • Lactoferrin

ASJC Scopus subject areas

  • Biochemistry

Cite this

Bovine lactoferrin and lactoferricin interfere with intracellular trafficking of Herpes simplex virus-1. / Marr, Alexandra K.; Jenssen, H.; Moniri, M. Roshan; Hancock, R. E W; Panté, N.

In: Biochimie, Vol. 91, No. 1, 01.2009, p. 160-164.

Research output: Contribution to journalArticle

Marr, Alexandra K. ; Jenssen, H. ; Moniri, M. Roshan ; Hancock, R. E W ; Panté, N. / Bovine lactoferrin and lactoferricin interfere with intracellular trafficking of Herpes simplex virus-1. In: Biochimie. 2009 ; Vol. 91, No. 1. pp. 160-164.
@article{2e3dc5fc294f446d90222e3cb1a48648,
title = "Bovine lactoferrin and lactoferricin interfere with intracellular trafficking of Herpes simplex virus-1",
abstract = "Although both lactoferrin (Lf), a component of the innate immune system of living organisms, and its N-terminal pepsin cleavage product lactoferricin (Lfcin) have anti-herpes activity, the precise mechanisms by which Lf and Lfcin bring about inhibition of herpes infections are not fully understood. In the present study, experiments were carried out to characterize the activity of bovine Lf and Lfcin (BLf and BLfcin) against the Herpes simplex virus-1 (HSV-1). HSV-1 cellular uptake and intracellular trafficking were studied by immunofluorescence microscopy. In comparison to the untreated infected control cells, both the BLf- and BLfcin-treated cells showed a significant reduction in HSV-1 cellular uptake. The few virus particles that were internalized appeared to have a delayed intracellular trafficking. Thus, in addition to their interference with the uptake of the virus into host cells, Lf and Lfcin also exert their antiviral effect intracellularly.",
keywords = "Antiviral, Herpes virus, HSV-1, Lactoferricin, Lactoferrin",
author = "Marr, {Alexandra K.} and H. Jenssen and Moniri, {M. Roshan} and Hancock, {R. E W} and N. Pant{\'e}",
year = "2009",
month = "1",
doi = "10.1016/j.biochi.2008.05.016",
language = "English",
volume = "91",
pages = "160--164",
journal = "Biochimie",
issn = "0300-9084",
publisher = "Elsevier",
number = "1",

}

TY - JOUR

T1 - Bovine lactoferrin and lactoferricin interfere with intracellular trafficking of Herpes simplex virus-1

AU - Marr, Alexandra K.

AU - Jenssen, H.

AU - Moniri, M. Roshan

AU - Hancock, R. E W

AU - Panté, N.

PY - 2009/1

Y1 - 2009/1

N2 - Although both lactoferrin (Lf), a component of the innate immune system of living organisms, and its N-terminal pepsin cleavage product lactoferricin (Lfcin) have anti-herpes activity, the precise mechanisms by which Lf and Lfcin bring about inhibition of herpes infections are not fully understood. In the present study, experiments were carried out to characterize the activity of bovine Lf and Lfcin (BLf and BLfcin) against the Herpes simplex virus-1 (HSV-1). HSV-1 cellular uptake and intracellular trafficking were studied by immunofluorescence microscopy. In comparison to the untreated infected control cells, both the BLf- and BLfcin-treated cells showed a significant reduction in HSV-1 cellular uptake. The few virus particles that were internalized appeared to have a delayed intracellular trafficking. Thus, in addition to their interference with the uptake of the virus into host cells, Lf and Lfcin also exert their antiviral effect intracellularly.

AB - Although both lactoferrin (Lf), a component of the innate immune system of living organisms, and its N-terminal pepsin cleavage product lactoferricin (Lfcin) have anti-herpes activity, the precise mechanisms by which Lf and Lfcin bring about inhibition of herpes infections are not fully understood. In the present study, experiments were carried out to characterize the activity of bovine Lf and Lfcin (BLf and BLfcin) against the Herpes simplex virus-1 (HSV-1). HSV-1 cellular uptake and intracellular trafficking were studied by immunofluorescence microscopy. In comparison to the untreated infected control cells, both the BLf- and BLfcin-treated cells showed a significant reduction in HSV-1 cellular uptake. The few virus particles that were internalized appeared to have a delayed intracellular trafficking. Thus, in addition to their interference with the uptake of the virus into host cells, Lf and Lfcin also exert their antiviral effect intracellularly.

KW - Antiviral

KW - Herpes virus

KW - HSV-1

KW - Lactoferricin

KW - Lactoferrin

UR - http://www.scopus.com/inward/record.url?scp=58149140247&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=58149140247&partnerID=8YFLogxK

U2 - 10.1016/j.biochi.2008.05.016

DO - 10.1016/j.biochi.2008.05.016

M3 - Article

C2 - 18573311

AN - SCOPUS:58149140247

VL - 91

SP - 160

EP - 164

JO - Biochimie

JF - Biochimie

SN - 0300-9084

IS - 1

ER -