Body fat free mass is associated with the serum metabolite profile in a population-based study

Carolin Jourdan, Ann Kristin Petersen, Christian Gieger, Angela Döring, Thomas Illig, Rui Wang-Sattler, Christa Meisinger, Annette Peters, Jerzy Adamski, Cornelia Prehn, Karsten Suhre, Elisabeth Altmaier, Gabi Kastenmüller, Werner Römisch-Margl, Fabian J. Theis, Jan Krumsiek, H. Erich Wichmann, Jakob Linseisen

Research output: Contribution to journalArticle

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Abstract

Objective: To characterise the influence of the fat free mass on the metabolite profile in serum samples from participants of the population-based KORA (Cooperative Health Research in the Region of Augsburg) S4 study. Subjects and Methods: Analyses were based on metabolite profile from 965 participants of the S4 and 890 weight-stable subjects of its seven-year follow-up study (KORA F4). 190 different serum metabolites were quantified in a targeted approach including amino acids, acylcarnitines, phosphatidylcholines (PCs), sphingomyelins and hexose. Associations between metabolite concentrations and the fat free mass index (FFMI) were analysed using adjusted linear regression models. To draw conclusions on enzymatic reactions, intra-metabolite class ratios were explored. Pairwise relationships among metabolites were investigated and illustrated by means of Gaussian graphical models (GGMs). Results: We found 339 significant associations between FFMI and various metabolites in KORA S4. Among the most prominent associations (p-values 4.75×10-16-8.95×10-06) with higher FFMI were increasing concentrations of the branched chained amino acids (BCAAs), ratios of BCAAs to glucogenic amino acids, and carnitine concentrations. For various PCs, a decrease in chain length or in saturation of the fatty acid moieties could be observed with increasing FFMI, as well as an overall shift from acyl-alkyl PCs to diacyl PCs. These findings were reproduced in KORA F4. The established GGMs supported the regression results and provided a comprehensive picture of the relationships between metabolites. In a sub-analysis, most of the discovered associations did not exist in obese subjects in contrast to non-obese subjects, possibly indicating derangements in skeletal muscle metabolism. Conclusion: A set of serum metabolites strongly associated with FFMI was identified and a network explaining the relationships among metabolites was established. These results offer a novel and more complete picture of the FFMI effects on serum metabolites in a data-driven network.

Original languageEnglish
Article numbere40009
JournalPLoS One
Volume7
Issue number6
DOIs
Publication statusPublished - 27 Jun 2012
Externally publishedYes

Fingerprint

lean body mass
Metabolites
Adipose Tissue
Fats
metabolites
Phosphatidylcholines
Serum
Population
Amino Acids
phosphatidylcholines
lipids
Linear Models
amino acids
Hexoses
Sphingomyelins
Skeletal Muscle
Fatty Acids
sphingomyelins
carnitine
enzymatic reactions

ASJC Scopus subject areas

  • Medicine(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

Jourdan, C., Petersen, A. K., Gieger, C., Döring, A., Illig, T., Wang-Sattler, R., ... Linseisen, J. (2012). Body fat free mass is associated with the serum metabolite profile in a population-based study. PLoS One, 7(6), [e40009]. https://doi.org/10.1371/journal.pone.0040009

Body fat free mass is associated with the serum metabolite profile in a population-based study. / Jourdan, Carolin; Petersen, Ann Kristin; Gieger, Christian; Döring, Angela; Illig, Thomas; Wang-Sattler, Rui; Meisinger, Christa; Peters, Annette; Adamski, Jerzy; Prehn, Cornelia; Suhre, Karsten; Altmaier, Elisabeth; Kastenmüller, Gabi; Römisch-Margl, Werner; Theis, Fabian J.; Krumsiek, Jan; Wichmann, H. Erich; Linseisen, Jakob.

In: PLoS One, Vol. 7, No. 6, e40009, 27.06.2012.

Research output: Contribution to journalArticle

Jourdan, C, Petersen, AK, Gieger, C, Döring, A, Illig, T, Wang-Sattler, R, Meisinger, C, Peters, A, Adamski, J, Prehn, C, Suhre, K, Altmaier, E, Kastenmüller, G, Römisch-Margl, W, Theis, FJ, Krumsiek, J, Wichmann, HE & Linseisen, J 2012, 'Body fat free mass is associated with the serum metabolite profile in a population-based study', PLoS One, vol. 7, no. 6, e40009. https://doi.org/10.1371/journal.pone.0040009
Jourdan C, Petersen AK, Gieger C, Döring A, Illig T, Wang-Sattler R et al. Body fat free mass is associated with the serum metabolite profile in a population-based study. PLoS One. 2012 Jun 27;7(6). e40009. https://doi.org/10.1371/journal.pone.0040009
Jourdan, Carolin ; Petersen, Ann Kristin ; Gieger, Christian ; Döring, Angela ; Illig, Thomas ; Wang-Sattler, Rui ; Meisinger, Christa ; Peters, Annette ; Adamski, Jerzy ; Prehn, Cornelia ; Suhre, Karsten ; Altmaier, Elisabeth ; Kastenmüller, Gabi ; Römisch-Margl, Werner ; Theis, Fabian J. ; Krumsiek, Jan ; Wichmann, H. Erich ; Linseisen, Jakob. / Body fat free mass is associated with the serum metabolite profile in a population-based study. In: PLoS One. 2012 ; Vol. 7, No. 6.
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