Blood transcriptional fingerprints to assess the immune status of human subjects

Damien J. Chaussabel, Nicole Baldwin, Derek Blankenship, Charles Quinn, Esperanza Anguiano, Octavio Ramilo, Ganjana Lertmemongkolchai, Virginia Pascual, Jacques Banchereau

Research output: Chapter in Book/Report/Conference proceedingChapter

1 Citation (Scopus)

Abstract

The blood transcriptome affords a comprehensive view of the status of the human immune system. Global changes in transcript abundance have been measured in the blood of patients with a wide range of diseases. This chapter presents an overview of the advances that have led to the identification of therapeutic targets and biomarker signatures in the field of autoimmunity and infectious disease. It also provides technology and data analysis primers as means of introducing blood transcriptome research to a broad readership. Specifically, we compare microarrays with some of the most recent digital gene expression profiling technologies available to date, including RNA sequencing. Furthermore, in addition to the basic steps involved in the analysis of microarray data we also present more advanced data mining approaches for blood transcriptional fingerprinting.

Original languageEnglish
Title of host publicationImmunologic Signatures of Rejection
PublisherSpringer New York
Pages105-125
Number of pages21
ISBN (Electronic)9781441972194
ISBN (Print)9781441972187
DOIs
Publication statusPublished - 2011
Externally publishedYes

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ASJC Scopus subject areas

  • Medicine(all)

Cite this

Chaussabel, D. J., Baldwin, N., Blankenship, D., Quinn, C., Anguiano, E., Ramilo, O., Lertmemongkolchai, G., Pascual, V., & Banchereau, J. (2011). Blood transcriptional fingerprints to assess the immune status of human subjects. In Immunologic Signatures of Rejection (pp. 105-125). Springer New York. https://doi.org/10.1007/978-1-4419-7219-4_8