Blockade of CC chemokine receptor 5 (CCR5)-tropic human immunodeficiency virus-1 replication in human lymphoid tissue by CC chemokines

Leonid B. Margolis, Svetlana Glushakova, Jean-Charles B. Grivel, Philip M. Murphy

Research output: Contribution to journalArticle

45 Citations (Scopus)

Abstract

The CC chemokines MIP-1α, MIP-1β, and RANTES suppress replication of certain HIV-1 strains in cultured PBMC and T cell lines by blocking interaction of gp120 with CC chemokine receptor 5 (CCR5). However, the same chemokines can enhance HIV-1 replication in cultured macrophages. The net effect of chemokines on HIV-1 infection in intact lymphoid tissue, the major reservoir of HIV-1 in vivo, is unknown and unpredictable since the tissue contains both T lymphocytes and macrophages. Here we show that exogenous MIP- 1α, MIP-1β, and RANTES markedly suppressed replication of CCR5-tropic HIV- 1 strains in blocks of human lymphoid tissue infected ex vivo. Moreover, endogenous MIP-1α, MIP-1β, and RANTES were upregulated in tissues infected ex vivo with CXC chemokine receptor 4-tropic but not CCR5-tropic HIV-1. Such an upregulation may contribute to the virus phenotype shift in the course of HIV disease in vivo.

Original languageEnglish
Pages (from-to)1876-1880
Number of pages5
JournalJournal of Clinical Investigation
Volume101
Issue number9
Publication statusPublished - 1 May 1998
Externally publishedYes

Fingerprint

CCR5 Receptors
CC Chemokines
Lymphoid Tissue
Virus Replication
HIV-1
Chemokine CCL5
Chemokines
Macrophages
CXCR4 Receptors
T-Lymphocytes
HIV Infections
Cultured Cells
Up-Regulation
HIV
Viruses
Phenotype
Cell Line

Keywords

  • AIDS
  • Chemokine
  • HIV
  • Leukocyte
  • Receptor

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Blockade of CC chemokine receptor 5 (CCR5)-tropic human immunodeficiency virus-1 replication in human lymphoid tissue by CC chemokines. / Margolis, Leonid B.; Glushakova, Svetlana; Grivel, Jean-Charles B.; Murphy, Philip M.

In: Journal of Clinical Investigation, Vol. 101, No. 9, 01.05.1998, p. 1876-1880.

Research output: Contribution to journalArticle

@article{813b175c386948e68a1b2de5bee814d0,
title = "Blockade of CC chemokine receptor 5 (CCR5)-tropic human immunodeficiency virus-1 replication in human lymphoid tissue by CC chemokines",
abstract = "The CC chemokines MIP-1α, MIP-1β, and RANTES suppress replication of certain HIV-1 strains in cultured PBMC and T cell lines by blocking interaction of gp120 with CC chemokine receptor 5 (CCR5). However, the same chemokines can enhance HIV-1 replication in cultured macrophages. The net effect of chemokines on HIV-1 infection in intact lymphoid tissue, the major reservoir of HIV-1 in vivo, is unknown and unpredictable since the tissue contains both T lymphocytes and macrophages. Here we show that exogenous MIP- 1α, MIP-1β, and RANTES markedly suppressed replication of CCR5-tropic HIV- 1 strains in blocks of human lymphoid tissue infected ex vivo. Moreover, endogenous MIP-1α, MIP-1β, and RANTES were upregulated in tissues infected ex vivo with CXC chemokine receptor 4-tropic but not CCR5-tropic HIV-1. Such an upregulation may contribute to the virus phenotype shift in the course of HIV disease in vivo.",
keywords = "AIDS, Chemokine, HIV, Leukocyte, Receptor",
author = "Margolis, {Leonid B.} and Svetlana Glushakova and Grivel, {Jean-Charles B.} and Murphy, {Philip M.}",
year = "1998",
month = "5",
day = "1",
language = "English",
volume = "101",
pages = "1876--1880",
journal = "Journal of Clinical Investigation",
issn = "0021-9738",
publisher = "The American Society for Clinical Investigation",
number = "9",

}

TY - JOUR

T1 - Blockade of CC chemokine receptor 5 (CCR5)-tropic human immunodeficiency virus-1 replication in human lymphoid tissue by CC chemokines

AU - Margolis, Leonid B.

AU - Glushakova, Svetlana

AU - Grivel, Jean-Charles B.

AU - Murphy, Philip M.

PY - 1998/5/1

Y1 - 1998/5/1

N2 - The CC chemokines MIP-1α, MIP-1β, and RANTES suppress replication of certain HIV-1 strains in cultured PBMC and T cell lines by blocking interaction of gp120 with CC chemokine receptor 5 (CCR5). However, the same chemokines can enhance HIV-1 replication in cultured macrophages. The net effect of chemokines on HIV-1 infection in intact lymphoid tissue, the major reservoir of HIV-1 in vivo, is unknown and unpredictable since the tissue contains both T lymphocytes and macrophages. Here we show that exogenous MIP- 1α, MIP-1β, and RANTES markedly suppressed replication of CCR5-tropic HIV- 1 strains in blocks of human lymphoid tissue infected ex vivo. Moreover, endogenous MIP-1α, MIP-1β, and RANTES were upregulated in tissues infected ex vivo with CXC chemokine receptor 4-tropic but not CCR5-tropic HIV-1. Such an upregulation may contribute to the virus phenotype shift in the course of HIV disease in vivo.

AB - The CC chemokines MIP-1α, MIP-1β, and RANTES suppress replication of certain HIV-1 strains in cultured PBMC and T cell lines by blocking interaction of gp120 with CC chemokine receptor 5 (CCR5). However, the same chemokines can enhance HIV-1 replication in cultured macrophages. The net effect of chemokines on HIV-1 infection in intact lymphoid tissue, the major reservoir of HIV-1 in vivo, is unknown and unpredictable since the tissue contains both T lymphocytes and macrophages. Here we show that exogenous MIP- 1α, MIP-1β, and RANTES markedly suppressed replication of CCR5-tropic HIV- 1 strains in blocks of human lymphoid tissue infected ex vivo. Moreover, endogenous MIP-1α, MIP-1β, and RANTES were upregulated in tissues infected ex vivo with CXC chemokine receptor 4-tropic but not CCR5-tropic HIV-1. Such an upregulation may contribute to the virus phenotype shift in the course of HIV disease in vivo.

KW - AIDS

KW - Chemokine

KW - HIV

KW - Leukocyte

KW - Receptor

UR - http://www.scopus.com/inward/record.url?scp=0032080672&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0032080672&partnerID=8YFLogxK

M3 - Article

VL - 101

SP - 1876

EP - 1880

JO - Journal of Clinical Investigation

JF - Journal of Clinical Investigation

SN - 0021-9738

IS - 9

ER -