Biosynthesis and polarized distribution of neutral endopeptidase in primary cultures of kidney proximal tubule cells

F. Jalal, Mohammed Dehbi, A. Berteloot, P. Crine

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

When cultured in defined medium, kidney proximal convoluted tubule (PCT) cells form a homogeneous population and retain a number of differentiated functions. To characterize this cell system further as a functional model of epithelial polarity, we investigated the biogenic pathway of neutral endopeptidase (NEP), one of the most abundant microvillar membrane proteins in intestinal and kidney cells. We showed that, in contrast with some tumoral cell lines, RNA extracted from PCT cells shows the presence of a single mRNA species encoding NEP. Pulse-chase studies followed by selective immunoprecipitation of NEP molecules present either at the cell surface or in intracellular cell compartments showed that newly synthesized NEP molecules reached the cell surface as early as 30 min after the beginning of the chase with maximum cell surface expression at 60 min. When grown on semipermeable supports, PCT cells were found to target NEP exclusively to the apical plasma membrane. Similar results have been described using MDCK cells to study targeting of recombinant NEP. Thus primary cultures of PCT cells represent a new model with which to investigate the biogenic pathway of endogenous proteins in native epithelial cells.

Original languageEnglish
Pages (from-to)669-674
Number of pages6
JournalBiochemical Journal
Volume302
Issue number3
Publication statusPublished - 1 Jan 1994
Externally publishedYes

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Neprilysin
Proximal Kidney Tubule
Biosynthesis
Molecules
Cell membranes
Membrane Proteins
Cells
Madin Darby Canine Kidney Cells
RNA
Messenger RNA
Immunoprecipitation
Epithelial Cells
Cell Membrane
Proteins
Kidney
Cell Line

ASJC Scopus subject areas

  • Biochemistry

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Biosynthesis and polarized distribution of neutral endopeptidase in primary cultures of kidney proximal tubule cells. / Jalal, F.; Dehbi, Mohammed; Berteloot, A.; Crine, P.

In: Biochemical Journal, Vol. 302, No. 3, 01.01.1994, p. 669-674.

Research output: Contribution to journalArticle

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