Biohydrogen production from sugarcane bagasse hydrolysate: effects of pH, S/X, Fe2+, and magnetite nanoparticles

Karen Reddy, Mahmoud Nasr, Sheena Kumari, Santhosh Kumar, Sanjay Kumar Gupta, Abimbola Motunrayo Enitan, Faizal Bux

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Batch dark fermentation experiments were conducted to investigate the effects of initial pH, substrate-to-biomass (S/X) ratio, and concentrations of Fe2+ and magnetite nanoparticles on biohydrogen production from sugarcane bagasse (SCB) hydrolysate. By applying the response surface methodology, the optimum condition of steam-acid hydrolysis was 0.64% (v/v) H2SO4 for 55.7 min, which obtained a sugar yield of 274 mg g−1. The maximum hydrogen yield (HY) of 0.874 mol (mol glucose−1) was detected at the optimum pH of 5.0 and S/X ratio of 0.5 g chemical oxygen demand (COD, g VSS−1). The addition of Fe2+ 200 mg L−1 and magnetite nanoparticles 200 mg L−1 to the inoculum enhanced the HY by 62.1% and 69.6%, respectively. The kinetics of hydrogen production was estimated by fitting the experimental data to the modified Gompertz model. The inhibitory effects of adding Fe2+ and magnetite nanoparticles to the fermentative hydrogen production were examined by applying Andrew’s inhibition model. COD mass balance and full stoichiometric reactions, including soluble metabolic products, cell synthesis, and H2 production, indicated the reliability of the experimental results. A qPCR-based analysis was conducted to assess the microbial community structure using Enterobacteriaceae, Clostridium spp., and hydrogenase-specific gene activity. Results from the microbial analysis revealed the dominance of hydrogen producers in the inoculum immobilized on magnetite nanoparticles, followed by the inoculum supplemented with Fe2+ concentration. [Figure not available: see fulltext.]

Original languageEnglish
Pages (from-to)8790-8804
Number of pages15
JournalEnvironmental Science and Pollution Research
Volume24
Issue number9
DOIs
Publication statusPublished - 1 Mar 2017
Externally publishedYes

Fingerprint

Magnetite Nanoparticles
Magnetite nanoparticles
Saccharum
Bagasse
magnetite
Hydrogen
hydrogen
Hydrogen production
Hydrogenase
Clostridium
Chemical oxygen demand
Steam
Biological Oxygen Demand Analysis
Sugars
Fermentation
Hydrolysis
Enterobacteriaceae
Biomass
Reproducibility of Results
Genes

Keywords

  • Biohydrogen
  • Dark fermentation
  • Full stoichiometry
  • Hydrogen-producing microbes
  • Magnetite nanoparticles
  • Sugarcane bagasse hydrolysate

ASJC Scopus subject areas

  • Environmental Chemistry
  • Pollution
  • Health, Toxicology and Mutagenesis

Cite this

Biohydrogen production from sugarcane bagasse hydrolysate : effects of pH, S/X, Fe2+, and magnetite nanoparticles. / Reddy, Karen; Nasr, Mahmoud; Kumari, Sheena; Kumar, Santhosh; Gupta, Sanjay Kumar; Enitan, Abimbola Motunrayo; Bux, Faizal.

In: Environmental Science and Pollution Research, Vol. 24, No. 9, 01.03.2017, p. 8790-8804.

Research output: Contribution to journalArticle

Reddy, Karen ; Nasr, Mahmoud ; Kumari, Sheena ; Kumar, Santhosh ; Gupta, Sanjay Kumar ; Enitan, Abimbola Motunrayo ; Bux, Faizal. / Biohydrogen production from sugarcane bagasse hydrolysate : effects of pH, S/X, Fe2+, and magnetite nanoparticles. In: Environmental Science and Pollution Research. 2017 ; Vol. 24, No. 9. pp. 8790-8804.
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AU - Nasr, Mahmoud

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AU - Kumar, Santhosh

AU - Gupta, Sanjay Kumar

AU - Enitan, Abimbola Motunrayo

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AB - Batch dark fermentation experiments were conducted to investigate the effects of initial pH, substrate-to-biomass (S/X) ratio, and concentrations of Fe2+ and magnetite nanoparticles on biohydrogen production from sugarcane bagasse (SCB) hydrolysate. By applying the response surface methodology, the optimum condition of steam-acid hydrolysis was 0.64% (v/v) H2SO4 for 55.7 min, which obtained a sugar yield of 274 mg g−1. The maximum hydrogen yield (HY) of 0.874 mol (mol glucose−1) was detected at the optimum pH of 5.0 and S/X ratio of 0.5 g chemical oxygen demand (COD, g VSS−1). The addition of Fe2+ 200 mg L−1 and magnetite nanoparticles 200 mg L−1 to the inoculum enhanced the HY by 62.1% and 69.6%, respectively. The kinetics of hydrogen production was estimated by fitting the experimental data to the modified Gompertz model. The inhibitory effects of adding Fe2+ and magnetite nanoparticles to the fermentative hydrogen production were examined by applying Andrew’s inhibition model. COD mass balance and full stoichiometric reactions, including soluble metabolic products, cell synthesis, and H2 production, indicated the reliability of the experimental results. A qPCR-based analysis was conducted to assess the microbial community structure using Enterobacteriaceae, Clostridium spp., and hydrogenase-specific gene activity. Results from the microbial analysis revealed the dominance of hydrogen producers in the inoculum immobilized on magnetite nanoparticles, followed by the inoculum supplemented with Fe2+ concentration. [Figure not available: see fulltext.]

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