Binding of histones to Xenopus laevis ribosomal genes with different levels of expression

Stephan I. DIMITROV, Hilda N. TATEOSSYAN, Victor Yu STEFANOVSKY, Valya R. RUSSANOVA, Luchesar KARAGYOZOV, Iliya G. PASHEV

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

The association of ribosomal RNA genes with histones as a function of their expression has been studied in Xenopus laevis erythrocytes, where the genes are silent, and tadpoles at stage 40, where these genes are actively transcribed. Isolated nuclei were either treated with formaldehyde or irradiated with an ultraviolet laser to cross‐link proteins to DNA. The covalently linked protein‐DNA complexes were purified by centrifugation through CsCl and immunoprecipitated with antibodies against H1, H2A and H4. DNA from the precipitated complexes was analysed for the presence of ribosomal DNA sequences by hybridization to specific probes. The actively transcribed ribosomal genes from X. laevis embryos are associated with H1, H2A and H4 as are the non‐transcribed genes in the erythrocytes.

Original languageEnglish
Pages (from-to)977-981
Number of pages5
JournalEuropean Journal of Biochemistry
Volume204
Issue number3
DOIs
Publication statusPublished - 1992
Externally publishedYes

Fingerprint

Xenopus laevis
Histones
Genes
Erythrocytes
DNA
Ultraviolet lasers
Ribosomal DNA
Centrifugation
rRNA Genes
Ribosomal RNA
Formaldehyde
DNA sequences
Larva
Lasers
Embryonic Structures
Antibodies
Proteins

ASJC Scopus subject areas

  • Biochemistry

Cite this

Binding of histones to Xenopus laevis ribosomal genes with different levels of expression. / DIMITROV, Stephan I.; TATEOSSYAN, Hilda N.; STEFANOVSKY, Victor Yu; RUSSANOVA, Valya R.; KARAGYOZOV, Luchesar; PASHEV, Iliya G.

In: European Journal of Biochemistry, Vol. 204, No. 3, 1992, p. 977-981.

Research output: Contribution to journalArticle

DIMITROV, Stephan I. ; TATEOSSYAN, Hilda N. ; STEFANOVSKY, Victor Yu ; RUSSANOVA, Valya R. ; KARAGYOZOV, Luchesar ; PASHEV, Iliya G. / Binding of histones to Xenopus laevis ribosomal genes with different levels of expression. In: European Journal of Biochemistry. 1992 ; Vol. 204, No. 3. pp. 977-981.
@article{841704f7b6274a3f91bf5c3ba44987ed,
title = "Binding of histones to Xenopus laevis ribosomal genes with different levels of expression",
abstract = "The association of ribosomal RNA genes with histones as a function of their expression has been studied in Xenopus laevis erythrocytes, where the genes are silent, and tadpoles at stage 40, where these genes are actively transcribed. Isolated nuclei were either treated with formaldehyde or irradiated with an ultraviolet laser to cross‐link proteins to DNA. The covalently linked protein‐DNA complexes were purified by centrifugation through CsCl and immunoprecipitated with antibodies against H1, H2A and H4. DNA from the precipitated complexes was analysed for the presence of ribosomal DNA sequences by hybridization to specific probes. The actively transcribed ribosomal genes from X. laevis embryos are associated with H1, H2A and H4 as are the non‐transcribed genes in the erythrocytes.",
author = "DIMITROV, {Stephan I.} and TATEOSSYAN, {Hilda N.} and STEFANOVSKY, {Victor Yu} and RUSSANOVA, {Valya R.} and Luchesar KARAGYOZOV and PASHEV, {Iliya G.}",
year = "1992",
doi = "10.1111/j.1432-1033.1992.tb16719.x",
language = "English",
volume = "204",
pages = "977--981",
journal = "FEBS Journal",
issn = "1742-464X",
publisher = "Wiley-Blackwell",
number = "3",

}

TY - JOUR

T1 - Binding of histones to Xenopus laevis ribosomal genes with different levels of expression

AU - DIMITROV, Stephan I.

AU - TATEOSSYAN, Hilda N.

AU - STEFANOVSKY, Victor Yu

AU - RUSSANOVA, Valya R.

AU - KARAGYOZOV, Luchesar

AU - PASHEV, Iliya G.

PY - 1992

Y1 - 1992

N2 - The association of ribosomal RNA genes with histones as a function of their expression has been studied in Xenopus laevis erythrocytes, where the genes are silent, and tadpoles at stage 40, where these genes are actively transcribed. Isolated nuclei were either treated with formaldehyde or irradiated with an ultraviolet laser to cross‐link proteins to DNA. The covalently linked protein‐DNA complexes were purified by centrifugation through CsCl and immunoprecipitated with antibodies against H1, H2A and H4. DNA from the precipitated complexes was analysed for the presence of ribosomal DNA sequences by hybridization to specific probes. The actively transcribed ribosomal genes from X. laevis embryos are associated with H1, H2A and H4 as are the non‐transcribed genes in the erythrocytes.

AB - The association of ribosomal RNA genes with histones as a function of their expression has been studied in Xenopus laevis erythrocytes, where the genes are silent, and tadpoles at stage 40, where these genes are actively transcribed. Isolated nuclei were either treated with formaldehyde or irradiated with an ultraviolet laser to cross‐link proteins to DNA. The covalently linked protein‐DNA complexes were purified by centrifugation through CsCl and immunoprecipitated with antibodies against H1, H2A and H4. DNA from the precipitated complexes was analysed for the presence of ribosomal DNA sequences by hybridization to specific probes. The actively transcribed ribosomal genes from X. laevis embryos are associated with H1, H2A and H4 as are the non‐transcribed genes in the erythrocytes.

UR - http://www.scopus.com/inward/record.url?scp=0026568509&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0026568509&partnerID=8YFLogxK

U2 - 10.1111/j.1432-1033.1992.tb16719.x

DO - 10.1111/j.1432-1033.1992.tb16719.x

M3 - Article

VL - 204

SP - 977

EP - 981

JO - FEBS Journal

JF - FEBS Journal

SN - 1742-464X

IS - 3

ER -