Baseline levels and temporal stability of 27 multiplexed serum cytokine concentrations in healthy subjects

Angelique Biancotto, Abigail Wank, Shira Perl, Wendell Cook, Matthew J. Olnes, Pradeep K. Dagur, J. Christopher Fuchs, Marc Langweiler, Ena Wang, J. Philip McCoy

Research output: Contribution to journalArticle

46 Citations (Scopus)

Abstract

Background: Cytokines are humoral molecules that elicit regulatory function in immunologic pathways. The level and type of cytokine production has become critical in distinguishing physiologic from pathologic immune conditions. Cytokine profiling has become an important biomarker discovery tool in monitoring of the immune system. However, the variations in cytokine levels in individual subjects over time in healthy individuals have not been extensively studied. In this study, we use multiplex bead arrays to evaluate 27 analytes in paired serum samples taken seven days apart from 144 healthy individuals in order to assess variations over a short time period. Methods: Fluorescent bead-based immunoassay (Luminex) was used to measure 27 analytes in serum samples. Measurements were performed on matched samples from 144 healthy donors. To assess inter-plate variability, one arbitrarily selected serum sample was analyzed on each of the first ten plates as bridge sample. Results: Using the bridge sample, we showed minimal inter-plate variations in the measurement of most analytes. In measurement of cytokines from the 144 patients at two time points, we found that three cytokines (IL-2, IL-15 and GM-CSF) were undetectable and five analytes (RANTES, MCP-1, VEGF, MIP-1β and PDGF-BB) showed significant difference in concentrations at Day 0 compared to Day 7. Conclusions: The current study demonstrated higher variations in cytokine levels among individuals than were observed for samples obtained one week apart from identical donors. These data suggest that a serum sample from each subject for use as a baseline measurement is a better control for clinical trials rather than sera from a paired cohort.

Original languageEnglish
Article numbere76091
JournalPLoS One
Volume8
Issue number12
DOIs
Publication statusPublished - 12 Dec 2013
Externally publishedYes

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Healthy Volunteers
cytokines
Cytokines
Serum
sampling
Tissue Donors
Interleukin-15
Chemokine CCL5
Immune system
Biomarkers
Granulocyte-Macrophage Colony-Stimulating Factor
Immunoassay
1-methylcyclopropene
Vascular Endothelial Growth Factor A
Interleukin-2
interleukin-2
immunoassays
Immune System
immune system
clinical trials

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Biancotto, A., Wank, A., Perl, S., Cook, W., Olnes, M. J., Dagur, P. K., ... McCoy, J. P. (2013). Baseline levels and temporal stability of 27 multiplexed serum cytokine concentrations in healthy subjects. PLoS One, 8(12), [e76091]. https://doi.org/10.1371/journal.pone.0076091

Baseline levels and temporal stability of 27 multiplexed serum cytokine concentrations in healthy subjects. / Biancotto, Angelique; Wank, Abigail; Perl, Shira; Cook, Wendell; Olnes, Matthew J.; Dagur, Pradeep K.; Fuchs, J. Christopher; Langweiler, Marc; Wang, Ena; McCoy, J. Philip.

In: PLoS One, Vol. 8, No. 12, e76091, 12.12.2013.

Research output: Contribution to journalArticle

Biancotto, A, Wank, A, Perl, S, Cook, W, Olnes, MJ, Dagur, PK, Fuchs, JC, Langweiler, M, Wang, E & McCoy, JP 2013, 'Baseline levels and temporal stability of 27 multiplexed serum cytokine concentrations in healthy subjects', PLoS One, vol. 8, no. 12, e76091. https://doi.org/10.1371/journal.pone.0076091
Biancotto, Angelique ; Wank, Abigail ; Perl, Shira ; Cook, Wendell ; Olnes, Matthew J. ; Dagur, Pradeep K. ; Fuchs, J. Christopher ; Langweiler, Marc ; Wang, Ena ; McCoy, J. Philip. / Baseline levels and temporal stability of 27 multiplexed serum cytokine concentrations in healthy subjects. In: PLoS One. 2013 ; Vol. 8, No. 12.
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