ATP-dependent chromatin remodeling is required for base excision repair in conventional but not in variant H2A.Bbd nucleosomes

Hervé Menoni, Didier Gasparutto, Ali Hamiche, Jean Cadet, Stefan Dimitrov, Philippe Bouvet, Dimitar Angelov

Research output: Contribution to journalArticle

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Abstract

In eukaryotes, base excision repair (BER) is responsible for the repair of oxidatively generated lesions. The mechanism of BER on naked DNA substrates has been studied in detail, but how it operates on chromatin remains unclear. Here we have studied the mechanism of BER by introducing a single 8-oxo-7,8-dihydroguanine (8-oxoG) lesion in the DNA of reconstituted positioned conventional and histone variant H2A.Bbd nucleosomes. We found that 8-oxoguanine DNA glycosylase, apurinic/apyrimidinic endonuclease, and polymerase β activities were strongly reduced in both types of nucleosomes. In conventional nucleosomes SWI/SNF stimulated the processing of 8-oxoG by each one of the three BER repair factors to efficiencies similar to those for naked DNA. Interestingly, SWI/SNF-induced remodeling, but not mobilization of conventional nucleosomes, was required to achieve this effect. A very weak effect of SWI/SNF on the 8-oxoG BER removal in H2A.Bbd histone variant nucleosomes was observed. The possible implications of our data for the understanding of in vivo mechanisms of BER are discussed.

Original languageEnglish
Pages (from-to)5949-5956
Number of pages8
JournalMolecular and Cellular Biology
Volume27
Issue number17
DOIs
Publication statusPublished - Sep 2007
Externally publishedYes

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Chromatin Assembly and Disassembly
Nucleosomes
DNA Repair
Adenosine Triphosphate
Histones
DNA
DNA-(Apurinic or Apyrimidinic Site) Lyase
DNA Glycosylases
Eukaryota
Chromatin
8-hydroxyguanine

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

Cite this

ATP-dependent chromatin remodeling is required for base excision repair in conventional but not in variant H2A.Bbd nucleosomes. / Menoni, Hervé; Gasparutto, Didier; Hamiche, Ali; Cadet, Jean; Dimitrov, Stefan; Bouvet, Philippe; Angelov, Dimitar.

In: Molecular and Cellular Biology, Vol. 27, No. 17, 09.2007, p. 5949-5956.

Research output: Contribution to journalArticle

Menoni, Hervé ; Gasparutto, Didier ; Hamiche, Ali ; Cadet, Jean ; Dimitrov, Stefan ; Bouvet, Philippe ; Angelov, Dimitar. / ATP-dependent chromatin remodeling is required for base excision repair in conventional but not in variant H2A.Bbd nucleosomes. In: Molecular and Cellular Biology. 2007 ; Vol. 27, No. 17. pp. 5949-5956.
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