Atomic model of the human cardiac muscle myosin filament

Hind A. Al-Khayat, Robert W. Kensler, John M. Squire, Steven B. Marston, Edward P. Morris

Research output: Contribution to journalArticle

61 Citations (Scopus)

Abstract

Of all the myosin filaments in muscle, the most important in terms of human health, and so far the least studied, are those in the human heart. Here we report a 3D single-particle analysis of electron micrograph images of negatively stained myosin filaments isolated from human cardiac muscle in the normal (undiseased) relaxed state. The resulting 28-Å resolution 3D reconstruction shows axial and azimuthal (no radial) myosin head perturbations within the 429-Å axial repeat, with rotations between successive 132 Å-, 148 Å-, and 149 Å-spaced crowns of heads close to 60° , 35° , and 25° (all would be 40° in an unperturbed three-stranded helix). We have defined the myosin head atomic arrangements within the three crown levels and have modeled the organization of myosin subfragment 2 and the possible locations of the 39 Å-spaced domains of titin and the cardiac isoform of myosin-binding protein-C on the surface of the myosin filament backbone. Best fits were obtained with head conformations on all crowns close to the structure of the two-headed myosin molecule of vertebrate chicken smooth muscle in the dephosphorylated relaxed state. Individual crowns show differences in head-pair tilts and subfragment 2 orientations, which, together with the observed perturbations, result in different intercrown head interactions, including one not reported before. Analysis of the interactions between the myosin heads, the cardiac isoform of myosin-binding protein-C, and titin will aid in understanding of the structural effects of mutations in these proteins known to be associated with human cardiomyopathies.

Original languageEnglish
Pages (from-to)318-323
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume110
Issue number1
DOIs
Publication statusPublished - 2 Jan 2013
Externally publishedYes

Fingerprint

Cardiac Myosins
Myosins
Crowns
Head
Connectin
Protein Isoforms
Myosin Subfragments
Cardiomyopathies
Smooth Muscle
Vertebrates
Chickens
Myocardium
Electrons
Muscles
Mutation
Health

Keywords

  • Electron microscopy
  • Human heart muscle
  • Image processing
  • Thick filaments 3D structure

ASJC Scopus subject areas

  • General

Cite this

Atomic model of the human cardiac muscle myosin filament. / Al-Khayat, Hind A.; Kensler, Robert W.; Squire, John M.; Marston, Steven B.; Morris, Edward P.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 110, No. 1, 02.01.2013, p. 318-323.

Research output: Contribution to journalArticle

Al-Khayat, Hind A. ; Kensler, Robert W. ; Squire, John M. ; Marston, Steven B. ; Morris, Edward P. / Atomic model of the human cardiac muscle myosin filament. In: Proceedings of the National Academy of Sciences of the United States of America. 2013 ; Vol. 110, No. 1. pp. 318-323.
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