Atom probe tomographic mapping directly reveals the atomic distribution of phosphorus in resin embedded ferritin

Daniel E. Perea, Jia Liu, Jonah Bartrand, Quinten Dicken, S. Theva Thevuthasan, Nigel D. Browning, James E. Evans

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Here we report the atomic-scale analysis of biological interfaces within the ferritin protein using atom probe tomography that is facilitated by an advanced specimen preparation approach. Embedding ferritin in an organic polymer resin lacking nitrogen provided chemical contrast to visualise atomic distributions and distinguish the inorganic-organic interface of the ferrihydrite mineral core and protein shell, as well as the organic-organic interface between the ferritin protein shell and embedding resin. In addition, we definitively show the atomic-scale distribution of phosphorus as being at the surface of the ferrihydrite mineral with the distribution of sodium mapped within the protein shell environment with an enhanced distribution at the mineral/protein interface. The sample preparation method is robust and can be directly extended to further enhance the study of biological, organic and inorganic nanomaterials relevant to health, energy or the environment.

Original languageEnglish
Article number22321
JournalScientific Reports
Volume6
DOIs
Publication statusPublished - 2016
Externally publishedYes

Fingerprint

Ferritins
Phosphorus
Resins
Atoms
Minerals
Proteins
Specimen preparation
Organic polymers
Nanostructured materials
Tomography
Nitrogen
Sodium
Health
ferrihydrite

ASJC Scopus subject areas

  • General

Cite this

Perea, D. E., Liu, J., Bartrand, J., Dicken, Q., Thevuthasan, S. T., Browning, N. D., & Evans, J. E. (2016). Atom probe tomographic mapping directly reveals the atomic distribution of phosphorus in resin embedded ferritin. Scientific Reports, 6, [22321]. https://doi.org/10.1038/srep22321

Atom probe tomographic mapping directly reveals the atomic distribution of phosphorus in resin embedded ferritin. / Perea, Daniel E.; Liu, Jia; Bartrand, Jonah; Dicken, Quinten; Thevuthasan, S. Theva; Browning, Nigel D.; Evans, James E.

In: Scientific Reports, Vol. 6, 22321, 2016.

Research output: Contribution to journalArticle

Perea, DE, Liu, J, Bartrand, J, Dicken, Q, Thevuthasan, ST, Browning, ND & Evans, JE 2016, 'Atom probe tomographic mapping directly reveals the atomic distribution of phosphorus in resin embedded ferritin', Scientific Reports, vol. 6, 22321. https://doi.org/10.1038/srep22321
Perea, Daniel E. ; Liu, Jia ; Bartrand, Jonah ; Dicken, Quinten ; Thevuthasan, S. Theva ; Browning, Nigel D. ; Evans, James E. / Atom probe tomographic mapping directly reveals the atomic distribution of phosphorus in resin embedded ferritin. In: Scientific Reports. 2016 ; Vol. 6.
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