Association of MMP but not TIMP-1 gene polymorphisms with recurrent aphthous stomatitis

J. A. Karasneh, M. E. Bani-Hani, A. M. Alkhateeb, A. F. Hassan, M. H. Thornhill

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Objectives: Recurrent aphthous stomatitis (RAS) is a common oral inflammatory disease induced by genetic and environmental factors. Gelatinases (MMP-2 and MMP-9) and their natural inhibitor TIMP-1 are active players in the inflammatory process. We aimed to determine whether inheritance of specific MMP-2, MMP-9, or TIMP-1 gene polymorphisms is associated with RAS susceptibility. Subjects and methods: Ninety-six RAS patients and 153 healthy controls were studied. Five polymorphisms were genotyped: rs17576, rs3918242, and rs11697325 in MMP-9, MMP-2 rs2285053, and TIMP-1 rs6609533. Association was assessed by logistic regression analysis after adjustment for confounding factors. Linkage disequilibrium (LD) was assessed using the Haploview program. Results: MMP-9 rs11697325 was significantly associated with RAS, with an increase in the AA genotype in patients, determined using χ2 analysis (OR = 2.3, P = 0.006) and adjusted regression analysis (OR = 3.1, P = 0.009). MMP-9 rs11697325 and rs17576 showed strong LD (D' = 0.95), with an increase in the AA haplotype (P = 0.023) and a decrease in the GA haplotype (P = 0.015) in patients. Conclusions: This is the first study to investigate the association of MMPs or TIMP-1 with RAS. We found a significant association between MMP-9 rs11697325 polymorphisms and RAS. Confirmatory studies in other populations and functional investigations are needed to determine the role of these genes in RAS.

Original languageEnglish
Pages (from-to)693-699
Number of pages7
JournalOral Diseases
Volume20
Issue number7
DOIs
Publication statusPublished - 1 Oct 2014
Externally publishedYes

Fingerprint

Tissue Inhibitor of Metalloproteinase-1
Matrix Metalloproteinases
Genes
Linkage Disequilibrium
Haplotypes
Mouth Diseases
Regression Analysis
Sutton disease 2
Gelatinases
Logistic Models
Genotype

Keywords

  • Matrix metalloproteinase
  • Polymorphism
  • Recurrent aphthous stomatitis
  • Tissue inhibitors of metalloproteinases

ASJC Scopus subject areas

  • Dentistry(all)
  • Otorhinolaryngology

Cite this

Karasneh, J. A., Bani-Hani, M. E., Alkhateeb, A. M., Hassan, A. F., & Thornhill, M. H. (2014). Association of MMP but not TIMP-1 gene polymorphisms with recurrent aphthous stomatitis. Oral Diseases, 20(7), 693-699. https://doi.org/10.1111/odi.12190

Association of MMP but not TIMP-1 gene polymorphisms with recurrent aphthous stomatitis. / Karasneh, J. A.; Bani-Hani, M. E.; Alkhateeb, A. M.; Hassan, A. F.; Thornhill, M. H.

In: Oral Diseases, Vol. 20, No. 7, 01.10.2014, p. 693-699.

Research output: Contribution to journalArticle

Karasneh, JA, Bani-Hani, ME, Alkhateeb, AM, Hassan, AF & Thornhill, MH 2014, 'Association of MMP but not TIMP-1 gene polymorphisms with recurrent aphthous stomatitis', Oral Diseases, vol. 20, no. 7, pp. 693-699. https://doi.org/10.1111/odi.12190
Karasneh JA, Bani-Hani ME, Alkhateeb AM, Hassan AF, Thornhill MH. Association of MMP but not TIMP-1 gene polymorphisms with recurrent aphthous stomatitis. Oral Diseases. 2014 Oct 1;20(7):693-699. https://doi.org/10.1111/odi.12190
Karasneh, J. A. ; Bani-Hani, M. E. ; Alkhateeb, A. M. ; Hassan, A. F. ; Thornhill, M. H. / Association of MMP but not TIMP-1 gene polymorphisms with recurrent aphthous stomatitis. In: Oral Diseases. 2014 ; Vol. 20, No. 7. pp. 693-699.
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abstract = "Objectives: Recurrent aphthous stomatitis (RAS) is a common oral inflammatory disease induced by genetic and environmental factors. Gelatinases (MMP-2 and MMP-9) and their natural inhibitor TIMP-1 are active players in the inflammatory process. We aimed to determine whether inheritance of specific MMP-2, MMP-9, or TIMP-1 gene polymorphisms is associated with RAS susceptibility. Subjects and methods: Ninety-six RAS patients and 153 healthy controls were studied. Five polymorphisms were genotyped: rs17576, rs3918242, and rs11697325 in MMP-9, MMP-2 rs2285053, and TIMP-1 rs6609533. Association was assessed by logistic regression analysis after adjustment for confounding factors. Linkage disequilibrium (LD) was assessed using the Haploview program. Results: MMP-9 rs11697325 was significantly associated with RAS, with an increase in the AA genotype in patients, determined using χ2 analysis (OR = 2.3, P = 0.006) and adjusted regression analysis (OR = 3.1, P = 0.009). MMP-9 rs11697325 and rs17576 showed strong LD (D' = 0.95), with an increase in the AA haplotype (P = 0.023) and a decrease in the GA haplotype (P = 0.015) in patients. Conclusions: This is the first study to investigate the association of MMPs or TIMP-1 with RAS. We found a significant association between MMP-9 rs11697325 polymorphisms and RAS. Confirmatory studies in other populations and functional investigations are needed to determine the role of these genes in RAS.",
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T1 - Association of MMP but not TIMP-1 gene polymorphisms with recurrent aphthous stomatitis

AU - Karasneh, J. A.

AU - Bani-Hani, M. E.

AU - Alkhateeb, A. M.

AU - Hassan, A. F.

AU - Thornhill, M. H.

PY - 2014/10/1

Y1 - 2014/10/1

N2 - Objectives: Recurrent aphthous stomatitis (RAS) is a common oral inflammatory disease induced by genetic and environmental factors. Gelatinases (MMP-2 and MMP-9) and their natural inhibitor TIMP-1 are active players in the inflammatory process. We aimed to determine whether inheritance of specific MMP-2, MMP-9, or TIMP-1 gene polymorphisms is associated with RAS susceptibility. Subjects and methods: Ninety-six RAS patients and 153 healthy controls were studied. Five polymorphisms were genotyped: rs17576, rs3918242, and rs11697325 in MMP-9, MMP-2 rs2285053, and TIMP-1 rs6609533. Association was assessed by logistic regression analysis after adjustment for confounding factors. Linkage disequilibrium (LD) was assessed using the Haploview program. Results: MMP-9 rs11697325 was significantly associated with RAS, with an increase in the AA genotype in patients, determined using χ2 analysis (OR = 2.3, P = 0.006) and adjusted regression analysis (OR = 3.1, P = 0.009). MMP-9 rs11697325 and rs17576 showed strong LD (D' = 0.95), with an increase in the AA haplotype (P = 0.023) and a decrease in the GA haplotype (P = 0.015) in patients. Conclusions: This is the first study to investigate the association of MMPs or TIMP-1 with RAS. We found a significant association between MMP-9 rs11697325 polymorphisms and RAS. Confirmatory studies in other populations and functional investigations are needed to determine the role of these genes in RAS.

AB - Objectives: Recurrent aphthous stomatitis (RAS) is a common oral inflammatory disease induced by genetic and environmental factors. Gelatinases (MMP-2 and MMP-9) and their natural inhibitor TIMP-1 are active players in the inflammatory process. We aimed to determine whether inheritance of specific MMP-2, MMP-9, or TIMP-1 gene polymorphisms is associated with RAS susceptibility. Subjects and methods: Ninety-six RAS patients and 153 healthy controls were studied. Five polymorphisms were genotyped: rs17576, rs3918242, and rs11697325 in MMP-9, MMP-2 rs2285053, and TIMP-1 rs6609533. Association was assessed by logistic regression analysis after adjustment for confounding factors. Linkage disequilibrium (LD) was assessed using the Haploview program. Results: MMP-9 rs11697325 was significantly associated with RAS, with an increase in the AA genotype in patients, determined using χ2 analysis (OR = 2.3, P = 0.006) and adjusted regression analysis (OR = 3.1, P = 0.009). MMP-9 rs11697325 and rs17576 showed strong LD (D' = 0.95), with an increase in the AA haplotype (P = 0.023) and a decrease in the GA haplotype (P = 0.015) in patients. Conclusions: This is the first study to investigate the association of MMPs or TIMP-1 with RAS. We found a significant association between MMP-9 rs11697325 polymorphisms and RAS. Confirmatory studies in other populations and functional investigations are needed to determine the role of these genes in RAS.

KW - Matrix metalloproteinase

KW - Polymorphism

KW - Recurrent aphthous stomatitis

KW - Tissue inhibitors of metalloproteinases

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