Association of caspase-9 and RUNX3 with inflammatory bowel disease

C. Guo, T. Ahmad, J. Beckly, J. R F Cummings, L. Hancock, A. Geremia, R. Cooney, S. Pathan, D. P. Jewell

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Previous linkage studies have identified a region at 1p36 as the susceptibility locus (IBD7) of inflammatory bowel disease (IBD). The objective of this study was to investigate whether polymorphisms of caspase-9 (CASP9) gene and RUNX3 are associated with IBD susceptibility and clinical phenotypes. We studied 555 Crohn's disease (CD) and 651 ulcerative colitis (UC) patients recruited from a single UK center. A total of 964 healthy Caucasian subjects were recruited as controls from general practitioner well person clinics in Oxfordshire. Fourteen single nucleotide polymorphisms (SNPs) of CASP9 and 11 SNPs of RUNX3 were genotyped using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) (homogenous MassEXTEND, hME, Sequenom™, Sequenom Inc., San Diego, CA). Linkage disequilibrium (LD) and haplotype association analysis were performed using 2ld and phase v2.0 software. No association of individual SNPs of CASP9 or RUNX3 with UC or CD was identified. The rs1052571 of CASP9 was associated with severe UC [P = 0.0034, odds ratio (OR) = 1.957, 95% confidence interval (CI) = 1.240-3.088]. Significant haplotype associations between CASP9 and IBD were identified, while no association of RUNX3 haplotypes with either UC or CD was found. Our findings suggested that CASP9 gene might be another IBD susceptibility gene.

Original languageEnglish
Pages (from-to)23-29
Number of pages7
JournalTissue Antigens
Volume77
Issue number1
DOIs
Publication statusPublished - Jan 2011
Externally publishedYes

Fingerprint

Caspase 9
Inflammatory Bowel Diseases
Ulcerative Colitis
Crohn Disease
Haplotypes
Single Nucleotide Polymorphism
Disease Susceptibility
Genes
Linkage Disequilibrium
General Practitioners
Mass Spectrometry
Healthy Volunteers
Lasers
Software
Odds Ratio
Confidence Intervals
Phenotype

Keywords

  • caspase 9
  • Crohn's disease
  • Inflammatory bowel disease
  • RUNX3
  • Ulcerative colitis

ASJC Scopus subject areas

  • Immunology
  • Immunology and Allergy
  • Genetics

Cite this

Guo, C., Ahmad, T., Beckly, J., Cummings, J. R. F., Hancock, L., Geremia, A., ... Jewell, D. P. (2011). Association of caspase-9 and RUNX3 with inflammatory bowel disease. Tissue Antigens, 77(1), 23-29. https://doi.org/10.1111/j.1399-0039.2010.01569.x

Association of caspase-9 and RUNX3 with inflammatory bowel disease. / Guo, C.; Ahmad, T.; Beckly, J.; Cummings, J. R F; Hancock, L.; Geremia, A.; Cooney, R.; Pathan, S.; Jewell, D. P.

In: Tissue Antigens, Vol. 77, No. 1, 01.2011, p. 23-29.

Research output: Contribution to journalArticle

Guo, C, Ahmad, T, Beckly, J, Cummings, JRF, Hancock, L, Geremia, A, Cooney, R, Pathan, S & Jewell, DP 2011, 'Association of caspase-9 and RUNX3 with inflammatory bowel disease', Tissue Antigens, vol. 77, no. 1, pp. 23-29. https://doi.org/10.1111/j.1399-0039.2010.01569.x
Guo C, Ahmad T, Beckly J, Cummings JRF, Hancock L, Geremia A et al. Association of caspase-9 and RUNX3 with inflammatory bowel disease. Tissue Antigens. 2011 Jan;77(1):23-29. https://doi.org/10.1111/j.1399-0039.2010.01569.x
Guo, C. ; Ahmad, T. ; Beckly, J. ; Cummings, J. R F ; Hancock, L. ; Geremia, A. ; Cooney, R. ; Pathan, S. ; Jewell, D. P. / Association of caspase-9 and RUNX3 with inflammatory bowel disease. In: Tissue Antigens. 2011 ; Vol. 77, No. 1. pp. 23-29.
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