Association of caspase-9 and RUNX3 with inflammatory bowel disease

C. Guo, T. Ahmad, J. Beckly, J. R.F. Cummings, L. Hancock, A. Geremia, R. Cooney, S. Pathan, D. P. Jewell

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    9 Citations (Scopus)

    Abstract

    Previous linkage studies have identified a region at 1p36 as the susceptibility locus (IBD7) of inflammatory bowel disease (IBD). The objective of this study was to investigate whether polymorphisms of caspase-9 (CASP9) gene and RUNX3 are associated with IBD susceptibility and clinical phenotypes. We studied 555 Crohn's disease (CD) and 651 ulcerative colitis (UC) patients recruited from a single UK center. A total of 964 healthy Caucasian subjects were recruited as controls from general practitioner well person clinics in Oxfordshire. Fourteen single nucleotide polymorphisms (SNPs) of CASP9 and 11 SNPs of RUNX3 were genotyped using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) (homogenous MassEXTEND, hME, Sequenom™, Sequenom Inc., San Diego, CA). Linkage disequilibrium (LD) and haplotype association analysis were performed using 2ld and phase v2.0 software. No association of individual SNPs of CASP9 or RUNX3 with UC or CD was identified. The rs1052571 of CASP9 was associated with severe UC [P = 0.0034, odds ratio (OR) = 1.957, 95% confidence interval (CI) = 1.240-3.088]. Significant haplotype associations between CASP9 and IBD were identified, while no association of RUNX3 haplotypes with either UC or CD was found. Our findings suggested that CASP9 gene might be another IBD susceptibility gene.

    Original languageEnglish
    Pages (from-to)23-29
    Number of pages7
    JournalTissue Antigens
    Volume77
    Issue number1
    DOIs
    Publication statusPublished - 1 Jan 2011

    Keywords

    • Crohn's disease
    • Inflammatory bowel disease
    • RUNX3
    • Ulcerative colitis
    • caspase 9

    ASJC Scopus subject areas

    • Immunology and Allergy
    • Immunology
    • Biochemistry
    • Genetics

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  • Cite this

    Guo, C., Ahmad, T., Beckly, J., Cummings, J. R. F., Hancock, L., Geremia, A., Cooney, R., Pathan, S., & Jewell, D. P. (2011). Association of caspase-9 and RUNX3 with inflammatory bowel disease. Tissue Antigens, 77(1), 23-29. https://doi.org/10.1111/j.1399-0039.2010.01569.x