Association in normal human fibroblasts of elevated levels of adenosine 3'

5'-monophosphate with a selective decrease in collagen production

B. J. Baum, J. Moss, S. D. Breul, Ronald Crystal

Research output: Contribution to journalArticle

69 Citations (Scopus)

Abstract

To evaluate the hypothesis that extracellular mediators may affect collagen production by mesenchymal cells via a cyclic AMP coordinated mechanism, normal human fibroblasts were exposed to a variety of agents (prostaglandin E1, isoproterenol, cholera toxin) which independently elevated intracellular cyclic AMP during a 6-h incubation. Concomitantly, each agent caused an average 47% reduction in the percentage of total protein synthesis represented by collagen, yet little change in other major extracellular proteins. Since no active collagenase was found in these cultures, these findings suggest cyclic AMP levels may modulate the differentiated state of normal fibroblasts with respect to collagen production.

Original languageEnglish
Pages (from-to)3391-3394
Number of pages4
JournalJournal of Biological Chemistry
Volume253
Issue number10
Publication statusPublished - 1 Jan 1978
Externally publishedYes

Fingerprint

Fibroblasts
Cyclic AMP
Adenosine
Collagen
Alprostadil
Cholera Toxin
Collagenases
Isoproterenol
Proteins

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Association in normal human fibroblasts of elevated levels of adenosine 3' : 5'-monophosphate with a selective decrease in collagen production. / Baum, B. J.; Moss, J.; Breul, S. D.; Crystal, Ronald.

In: Journal of Biological Chemistry, Vol. 253, No. 10, 01.01.1978, p. 3391-3394.

Research output: Contribution to journalArticle

@article{89332b3629cd4149ba0a9d82bc68fa41,
title = "Association in normal human fibroblasts of elevated levels of adenosine 3': 5'-monophosphate with a selective decrease in collagen production",
abstract = "To evaluate the hypothesis that extracellular mediators may affect collagen production by mesenchymal cells via a cyclic AMP coordinated mechanism, normal human fibroblasts were exposed to a variety of agents (prostaglandin E1, isoproterenol, cholera toxin) which independently elevated intracellular cyclic AMP during a 6-h incubation. Concomitantly, each agent caused an average 47{\%} reduction in the percentage of total protein synthesis represented by collagen, yet little change in other major extracellular proteins. Since no active collagenase was found in these cultures, these findings suggest cyclic AMP levels may modulate the differentiated state of normal fibroblasts with respect to collagen production.",
author = "Baum, {B. J.} and J. Moss and Breul, {S. D.} and Ronald Crystal",
year = "1978",
month = "1",
day = "1",
language = "English",
volume = "253",
pages = "3391--3394",
journal = "Journal of Biological Chemistry",
issn = "0021-9258",
publisher = "American Society for Biochemistry and Molecular Biology Inc.",
number = "10",

}

TY - JOUR

T1 - Association in normal human fibroblasts of elevated levels of adenosine 3'

T2 - 5'-monophosphate with a selective decrease in collagen production

AU - Baum, B. J.

AU - Moss, J.

AU - Breul, S. D.

AU - Crystal, Ronald

PY - 1978/1/1

Y1 - 1978/1/1

N2 - To evaluate the hypothesis that extracellular mediators may affect collagen production by mesenchymal cells via a cyclic AMP coordinated mechanism, normal human fibroblasts were exposed to a variety of agents (prostaglandin E1, isoproterenol, cholera toxin) which independently elevated intracellular cyclic AMP during a 6-h incubation. Concomitantly, each agent caused an average 47% reduction in the percentage of total protein synthesis represented by collagen, yet little change in other major extracellular proteins. Since no active collagenase was found in these cultures, these findings suggest cyclic AMP levels may modulate the differentiated state of normal fibroblasts with respect to collagen production.

AB - To evaluate the hypothesis that extracellular mediators may affect collagen production by mesenchymal cells via a cyclic AMP coordinated mechanism, normal human fibroblasts were exposed to a variety of agents (prostaglandin E1, isoproterenol, cholera toxin) which independently elevated intracellular cyclic AMP during a 6-h incubation. Concomitantly, each agent caused an average 47% reduction in the percentage of total protein synthesis represented by collagen, yet little change in other major extracellular proteins. Since no active collagenase was found in these cultures, these findings suggest cyclic AMP levels may modulate the differentiated state of normal fibroblasts with respect to collagen production.

UR - http://www.scopus.com/inward/record.url?scp=0017867012&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0017867012&partnerID=8YFLogxK

M3 - Article

VL - 253

SP - 3391

EP - 3394

JO - Journal of Biological Chemistry

JF - Journal of Biological Chemistry

SN - 0021-9258

IS - 10

ER -