Association analysis of HLA-Class II and Class III gene polymorphisms in the susceptibility to mediterranean visceral leishmaniasis

A. Meddeb-Garnaoui, S. Gritli, S. Garbouj, M. Ben Fadhel, R. El Kares, L. Mansour, B. Kaabi, Lotfi Chouchane, A. Ben Salah, K. Dellagi

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Abstract

HLA-DRB1, -DQB1, TNFα, TNFβ, HSP70-2 and HSP70-hom genetic polymorphisms were analyzed in 156 unrelated patients who developed mediterranean visceral leishmaniasis (MVL) due to Leishmania infantum, and 154 unrelated healthy controls, who have got asymptomatic infection with this parasite and were selected on the basis of a positive leishmanin skin test (LST). A significantly reduced frequency of HLA-DR2 was observed among MVL patients (16.1%), compared with controls (26.3%) (relative risk = 0.54; p = 0.04). HLA-DR2/DR13 as well as HLA-DQB1*0201/- genotype frequencies were significantly lower in patients vs controls (relapse rate = 0.17 and 0.46, respectively; p < 0.05). However, using Bonferroni correction, none of these associations remained significant. No association was found, between either the -308 base pair TNFα gene polymorphism or the NcoI polymorphism in the first intron of the TNFβ gene and susceptibility to MVL. Analysis of PstI and NcoI polymorphisms in the coding region of HSP70-2 and HSP70-hom genes, respectively, revealed a significantly higher frequency of homozygotes for the HSP70-2/PstI negative allele, among patients (21.8%) vs controls (12.6%) (relapse rate = 1.94; p = 0.04). Again, this result was not significant after using Bonferroni correction. These results do not support association between susceptibility to MVL and the MHC class II and class III loci analyzed in this study.

Original languageEnglish
Pages (from-to)509-517
Number of pages9
JournalHuman Immunology
Volume62
Issue number5
DOIs
Publication statusPublished - May 2001
Externally publishedYes

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Keywords

  • Candidate gene
  • DNA typing
  • Genetic susceptibility
  • HLA
  • Leishmaniasis
  • MHC genes
  • Tumor necrosis factor

ASJC Scopus subject areas

  • Immunology
  • Immunology and Allergy

Cite this

Meddeb-Garnaoui, A., Gritli, S., Garbouj, S., Ben Fadhel, M., El Kares, R., Mansour, L., Kaabi, B., Chouchane, L., Ben Salah, A., & Dellagi, K. (2001). Association analysis of HLA-Class II and Class III gene polymorphisms in the susceptibility to mediterranean visceral leishmaniasis. Human Immunology, 62(5), 509-517. https://doi.org/10.1016/S0198-8859(01)00237-3