Assessment of Parylene C thin films for heart valve tissue engineering

Isra Marei, Adrian Chester, Ivan Carubelli, Themistoklis Prodromakis, Tatiana Trantidou, Magdi H. Yacoub

    Research output: Contribution to journalArticle

    4 Citations (Scopus)

    Abstract

    Background: Scaffolds are a key component of tissue-engineered heart valves (TEHVs). Several approaches had been adopted in the design of scaffolds using both natural and synthetic resources. We have investigated the suitability of parylene C (PC), a vapor deposited polymeric material, for the use as a scaffold in TEHV. Aims: To evaluate the adsorption of extracellular matrix components onto plasma-activated PC and study the biocompatibility of PC by measuring cellular adhesion, viability, apoptosis, and phenotypic expression of valve endothelial and interstitial cells. Finally, the mechanical properties of PC were compared with those of native aortic valve cusp tissue. Methods: PC slides were plasma activated and then coated with gelatin, type I collagen, or fibronectin. Porcine pulmonary valve endothelial and interstitial cells were then grown on plasma oxidized PC with different types of coatings and their adhesion was observed after 20h of incubation. Cell viability was tested using the MTS assay, and apoptosis was estimated using TUNEL staining. The mechanical properties of PC and valve tissue were measured using a Bose Mechanical Tester. Finally, cell-seeded PC films were exposed to pulsatile pressure and aortic shear stress, respectively, to test their durability in a dynamic environment. Results: Our findings show that collagen and fibronectin could bind to plasma oxidized PC. Both valve endothelial and interstitial cells adhered to protein-coated ECM. PC had a profile of mechanical stiffness and ultimate tensile strength that were comparable with or in excess of those seen in porcine aortic valve cusps. Cells were still attached to PC films after 3 days of exposure to up to 50mmHg pulsatile pressure or aortic levels of shear stress. Conclusion: PC is a promising candidate for use as a scaffold in tissue engineering heart valves. Additional studies are required to determine both the durability and long-term performance of cell-seeded PC when in a similar hemodynamic environment to that of the aortic valve.

    Original languageEnglish
    Pages (from-to)2504-2514
    Number of pages11
    JournalTissue Engineering - Part A
    Volume21
    Issue number19-20
    DOIs
    Publication statusPublished - 1 Oct 2015

    Fingerprint

    Heart Valves
    Tissue Engineering
    Tissue engineering
    Tissue
    Plasmas
    Thin films
    Cell death
    Scaffolds (biology)
    Collagen
    Scaffolds
    Shear stress
    Durability
    Adhesion
    Military electronic countermeasures
    Mechanical properties
    Hemodynamics
    Biocompatibility
    Aortic Valve
    Assays
    Tensile strength

    ASJC Scopus subject areas

    • Bioengineering
    • Biochemistry
    • Biomedical Engineering
    • Biomaterials

    Cite this

    Marei, I., Chester, A., Carubelli, I., Prodromakis, T., Trantidou, T., & Yacoub, M. H. (2015). Assessment of Parylene C thin films for heart valve tissue engineering. Tissue Engineering - Part A, 21(19-20), 2504-2514. https://doi.org/10.1089/ten.tea.2014.0607

    Assessment of Parylene C thin films for heart valve tissue engineering. / Marei, Isra; Chester, Adrian; Carubelli, Ivan; Prodromakis, Themistoklis; Trantidou, Tatiana; Yacoub, Magdi H.

    In: Tissue Engineering - Part A, Vol. 21, No. 19-20, 01.10.2015, p. 2504-2514.

    Research output: Contribution to journalArticle

    Marei, I, Chester, A, Carubelli, I, Prodromakis, T, Trantidou, T & Yacoub, MH 2015, 'Assessment of Parylene C thin films for heart valve tissue engineering', Tissue Engineering - Part A, vol. 21, no. 19-20, pp. 2504-2514. https://doi.org/10.1089/ten.tea.2014.0607
    Marei I, Chester A, Carubelli I, Prodromakis T, Trantidou T, Yacoub MH. Assessment of Parylene C thin films for heart valve tissue engineering. Tissue Engineering - Part A. 2015 Oct 1;21(19-20):2504-2514. https://doi.org/10.1089/ten.tea.2014.0607
    Marei, Isra ; Chester, Adrian ; Carubelli, Ivan ; Prodromakis, Themistoklis ; Trantidou, Tatiana ; Yacoub, Magdi H. / Assessment of Parylene C thin films for heart valve tissue engineering. In: Tissue Engineering - Part A. 2015 ; Vol. 21, No. 19-20. pp. 2504-2514.
    @article{f0bf9c75362b4af69fe9f8b8e6719466,
    title = "Assessment of Parylene C thin films for heart valve tissue engineering",
    abstract = "Background: Scaffolds are a key component of tissue-engineered heart valves (TEHVs). Several approaches had been adopted in the design of scaffolds using both natural and synthetic resources. We have investigated the suitability of parylene C (PC), a vapor deposited polymeric material, for the use as a scaffold in TEHV. Aims: To evaluate the adsorption of extracellular matrix components onto plasma-activated PC and study the biocompatibility of PC by measuring cellular adhesion, viability, apoptosis, and phenotypic expression of valve endothelial and interstitial cells. Finally, the mechanical properties of PC were compared with those of native aortic valve cusp tissue. Methods: PC slides were plasma activated and then coated with gelatin, type I collagen, or fibronectin. Porcine pulmonary valve endothelial and interstitial cells were then grown on plasma oxidized PC with different types of coatings and their adhesion was observed after 20h of incubation. Cell viability was tested using the MTS assay, and apoptosis was estimated using TUNEL staining. The mechanical properties of PC and valve tissue were measured using a Bose Mechanical Tester. Finally, cell-seeded PC films were exposed to pulsatile pressure and aortic shear stress, respectively, to test their durability in a dynamic environment. Results: Our findings show that collagen and fibronectin could bind to plasma oxidized PC. Both valve endothelial and interstitial cells adhered to protein-coated ECM. PC had a profile of mechanical stiffness and ultimate tensile strength that were comparable with or in excess of those seen in porcine aortic valve cusps. Cells were still attached to PC films after 3 days of exposure to up to 50mmHg pulsatile pressure or aortic levels of shear stress. Conclusion: PC is a promising candidate for use as a scaffold in tissue engineering heart valves. Additional studies are required to determine both the durability and long-term performance of cell-seeded PC when in a similar hemodynamic environment to that of the aortic valve.",
    author = "Isra Marei and Adrian Chester and Ivan Carubelli and Themistoklis Prodromakis and Tatiana Trantidou and Yacoub, {Magdi H.}",
    year = "2015",
    month = "10",
    day = "1",
    doi = "10.1089/ten.tea.2014.0607",
    language = "English",
    volume = "21",
    pages = "2504--2514",
    journal = "Tissue Engineering - Part A.",
    issn = "1937-3341",
    publisher = "Mary Ann Liebert Inc.",
    number = "19-20",

    }

    TY - JOUR

    T1 - Assessment of Parylene C thin films for heart valve tissue engineering

    AU - Marei, Isra

    AU - Chester, Adrian

    AU - Carubelli, Ivan

    AU - Prodromakis, Themistoklis

    AU - Trantidou, Tatiana

    AU - Yacoub, Magdi H.

    PY - 2015/10/1

    Y1 - 2015/10/1

    N2 - Background: Scaffolds are a key component of tissue-engineered heart valves (TEHVs). Several approaches had been adopted in the design of scaffolds using both natural and synthetic resources. We have investigated the suitability of parylene C (PC), a vapor deposited polymeric material, for the use as a scaffold in TEHV. Aims: To evaluate the adsorption of extracellular matrix components onto plasma-activated PC and study the biocompatibility of PC by measuring cellular adhesion, viability, apoptosis, and phenotypic expression of valve endothelial and interstitial cells. Finally, the mechanical properties of PC were compared with those of native aortic valve cusp tissue. Methods: PC slides were plasma activated and then coated with gelatin, type I collagen, or fibronectin. Porcine pulmonary valve endothelial and interstitial cells were then grown on plasma oxidized PC with different types of coatings and their adhesion was observed after 20h of incubation. Cell viability was tested using the MTS assay, and apoptosis was estimated using TUNEL staining. The mechanical properties of PC and valve tissue were measured using a Bose Mechanical Tester. Finally, cell-seeded PC films were exposed to pulsatile pressure and aortic shear stress, respectively, to test their durability in a dynamic environment. Results: Our findings show that collagen and fibronectin could bind to plasma oxidized PC. Both valve endothelial and interstitial cells adhered to protein-coated ECM. PC had a profile of mechanical stiffness and ultimate tensile strength that were comparable with or in excess of those seen in porcine aortic valve cusps. Cells were still attached to PC films after 3 days of exposure to up to 50mmHg pulsatile pressure or aortic levels of shear stress. Conclusion: PC is a promising candidate for use as a scaffold in tissue engineering heart valves. Additional studies are required to determine both the durability and long-term performance of cell-seeded PC when in a similar hemodynamic environment to that of the aortic valve.

    AB - Background: Scaffolds are a key component of tissue-engineered heart valves (TEHVs). Several approaches had been adopted in the design of scaffolds using both natural and synthetic resources. We have investigated the suitability of parylene C (PC), a vapor deposited polymeric material, for the use as a scaffold in TEHV. Aims: To evaluate the adsorption of extracellular matrix components onto plasma-activated PC and study the biocompatibility of PC by measuring cellular adhesion, viability, apoptosis, and phenotypic expression of valve endothelial and interstitial cells. Finally, the mechanical properties of PC were compared with those of native aortic valve cusp tissue. Methods: PC slides were plasma activated and then coated with gelatin, type I collagen, or fibronectin. Porcine pulmonary valve endothelial and interstitial cells were then grown on plasma oxidized PC with different types of coatings and their adhesion was observed after 20h of incubation. Cell viability was tested using the MTS assay, and apoptosis was estimated using TUNEL staining. The mechanical properties of PC and valve tissue were measured using a Bose Mechanical Tester. Finally, cell-seeded PC films were exposed to pulsatile pressure and aortic shear stress, respectively, to test their durability in a dynamic environment. Results: Our findings show that collagen and fibronectin could bind to plasma oxidized PC. Both valve endothelial and interstitial cells adhered to protein-coated ECM. PC had a profile of mechanical stiffness and ultimate tensile strength that were comparable with or in excess of those seen in porcine aortic valve cusps. Cells were still attached to PC films after 3 days of exposure to up to 50mmHg pulsatile pressure or aortic levels of shear stress. Conclusion: PC is a promising candidate for use as a scaffold in tissue engineering heart valves. Additional studies are required to determine both the durability and long-term performance of cell-seeded PC when in a similar hemodynamic environment to that of the aortic valve.

    UR - http://www.scopus.com/inward/record.url?scp=84944050000&partnerID=8YFLogxK

    UR - http://www.scopus.com/inward/citedby.url?scp=84944050000&partnerID=8YFLogxK

    U2 - 10.1089/ten.tea.2014.0607

    DO - 10.1089/ten.tea.2014.0607

    M3 - Article

    AN - SCOPUS:84944050000

    VL - 21

    SP - 2504

    EP - 2514

    JO - Tissue Engineering - Part A.

    JF - Tissue Engineering - Part A.

    SN - 1937-3341

    IS - 19-20

    ER -