Assessment of Chinese medicinal herb metabolite profiles by UPLC-MS-based methodology for the detection of aristolochic acids

Shana Sunny Jacob, Norman William Smith, Cristina Legido-Quigley

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

In this study, aristolochic acid in different herbal medicines containing a mixture of varying herb species was identified through fingerprint pattern similarities. Aristolochic acid I and II are nephrotoxic compounds naturally present in the Aristolochia plant species that are commonly used in Chinese herbal medicines. Twenty-four commercially available herbal formulations were extracted into an aqueous solution and injected into a UPLC-MS system. All the samples were analysed by multiple reaction monitoring (MRM) to check for the presence of aristolochic acids I and II. The same samples were then fingerprinted using two different gradient methods and the chromatograms deconvoluted into retention time (RT) and masses for the chemicals present taking concentration into account. Statistical analysis of this data revealed that samples were highly heterogeneous, and that the main differences between the preparations were concentrations of polar compounds. A model was constructed where the samples could be separated into two groups differentiated by the presence of the two forms of aristolochic acid.

Original languageEnglish
Pages (from-to)1200-1206
Number of pages7
JournalJournal of Separation Science
Volume30
Issue number8
DOIs
Publication statusPublished - May 2007
Externally publishedYes

Fingerprint

Aristolochic Acids
Medicinal Plants
Metabolites
Acids
Herbal Medicine
Aristolochia
Statistical Data Interpretation
Gradient methods
Dermatoglyphics
Statistical methods
aristolochic acid I
Monitoring

Keywords

  • Chemometrics
  • Fingerprinting
  • Herbal medicines
  • UPLC-MS

ASJC Scopus subject areas

  • Molecular Medicine
  • Clinical Biochemistry
  • Chemical Engineering(all)
  • Filtration and Separation

Cite this

Assessment of Chinese medicinal herb metabolite profiles by UPLC-MS-based methodology for the detection of aristolochic acids. / Jacob, Shana Sunny; Smith, Norman William; Legido-Quigley, Cristina.

In: Journal of Separation Science, Vol. 30, No. 8, 05.2007, p. 1200-1206.

Research output: Contribution to journalArticle

Jacob, Shana Sunny ; Smith, Norman William ; Legido-Quigley, Cristina. / Assessment of Chinese medicinal herb metabolite profiles by UPLC-MS-based methodology for the detection of aristolochic acids. In: Journal of Separation Science. 2007 ; Vol. 30, No. 8. pp. 1200-1206.
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