Assessing the three-dimensional collagen network in soft tissues using contrast agents and high resolution micro-CT: Application to porcine iliac veins

Mathieu Nierenberger, Yves Rémond, Said Ahzi, Philippe Choquet

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

The assessment of the three-dimensional architecture of collagen fibers inside vessel walls constitutes one of the bases for building structural models for the description of the mechanical behavior of these tissues. Multiphoton microscopy allows for such observations, but is limited to volumes of around a thousand of microns. In the present work, we propose to observe the collagenous network of vascular tissues using micro-CT. To get a contrast, three staining solutions (phosphotungstic acid, phosphomolybdic acid and iodine potassium iodide) were tested. Two of these stains were showed to lead to similar results and to a satisfactory contrast within the tissue. A detailed observation of a small porcine iliac vein sample allowed assessing the collagen fibers orientations within the medial and adventitial layers of the vein. The vasa vasorum network, which is present inside the adventitia of the vein, was also observed. Finally, the demonstrated micro-CT staining technique for the three-dimensional observation of thin soft tissues samples, like vein walls, contributes to the assessment of their structure at different scales while keeping a global overview of the tissue.

Original languageEnglish
Pages (from-to)425-433
Number of pages9
JournalComptes Rendus - Biologies
Volume338
Issue number7
DOIs
Publication statusPublished - 1 Jul 2015
Externally publishedYes

Fingerprint

Iliac Vein
micro-computed tomography
Contrast Media
collagen
Swine
Collagen
Tissue
swine
Veins
Adventitia
phosphotungstic acid
potassium iodide
Vasa Vasorum
Observation
Phosphotungstic Acid
Staining and Labeling
xylem vessels
vascular tissues
iodine
Structural Models

Keywords

  • Collagen
  • Three-dimensional imaging
  • Tissue stains
  • Vasa vasorum
  • Vein wall
  • X-Ray microtomography

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)
  • Medicine(all)

Cite this

Assessing the three-dimensional collagen network in soft tissues using contrast agents and high resolution micro-CT : Application to porcine iliac veins. / Nierenberger, Mathieu; Rémond, Yves; Ahzi, Said; Choquet, Philippe.

In: Comptes Rendus - Biologies, Vol. 338, No. 7, 01.07.2015, p. 425-433.

Research output: Contribution to journalArticle

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