Assessing humoral immune response of 4 recombinant antigens for serodiagnosis of tuberculosis

Paramanandhan Pukazhvanthen, Deenadayalan Anbarasu, Basirudeen S. Kabeer, Alamelu Raja, Mahavir Singh

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Serodiagnostic potential of four recombinant proteins (38 kDa[Rv0934], MPT64[Rv1980c], Adk[Rv0733], and BfrB[Rv3874]) was evaluated in Healthy control subjects (HCS), Healthy household contacts (HHC), Pulmonary tuberculosis patients (PTB), and Human immuno deficiency virus & Tuberculosis co-infected patients (HIV-TB). All the antigens tested individually for the detection of serum IgG by indirect ELISA. All the four antigens have a significantly higher antibody response in PTB compared to healthy controls (P < 0.05). The sensitivity of individual antigens ranged from 20% to 52.5% for the prefixed specificity of 95%. When results of all 4 antigens were combined the sensitivity was increased to 75% and specificity was reduced 89% in HCS. In smear- and culture-positive (S+C+) PTB, four antigen combination gives maximum sensitivity (89.6%) with 89% specificity. In smear negative culture negative (S-C+) PTB, three antigen combination (38 kDa with MPT64 and BfrB) gives maximum sensitivity (69.5%) and specificity (91.6%). In HIV-TB, 4 antigen combinations give the maximum sensitivity of 51.2% with 89% specificity. Combining serology (Four antigen combination) with smear was able to increase the sensitivity from 70% to 92.5% in culture positive PTB. So, we propose that this serology test can be used as adjunct test along with smear for rapid diagnosis of PTB.

Original languageEnglish
Pages (from-to)622-633
Number of pages12
JournalTuberculosis
Volume94
Issue number6
DOIs
Publication statusPublished - 1 Dec 2014
Externally publishedYes

Fingerprint

Serologic Tests
Humoral Immunity
Tuberculosis
Pulmonary Tuberculosis
Antigens
Serology
Healthy Volunteers
HIV
Recombinant Proteins
Antibody Formation
Immunoglobulin G
Enzyme-Linked Immunosorbent Assay
Viruses
Sensitivity and Specificity
Serum

Keywords

  • Adk
  • BfrB
  • HIV-TB
  • MPT64
  • Serodiagnosis
  • Tuberculosis

ASJC Scopus subject areas

  • Microbiology
  • Immunology
  • Infectious Diseases
  • Microbiology (medical)

Cite this

Assessing humoral immune response of 4 recombinant antigens for serodiagnosis of tuberculosis. / Pukazhvanthen, Paramanandhan; Anbarasu, Deenadayalan; Kabeer, Basirudeen S.; Raja, Alamelu; Singh, Mahavir.

In: Tuberculosis, Vol. 94, No. 6, 01.12.2014, p. 622-633.

Research output: Contribution to journalArticle

Pukazhvanthen, Paramanandhan ; Anbarasu, Deenadayalan ; Kabeer, Basirudeen S. ; Raja, Alamelu ; Singh, Mahavir. / Assessing humoral immune response of 4 recombinant antigens for serodiagnosis of tuberculosis. In: Tuberculosis. 2014 ; Vol. 94, No. 6. pp. 622-633.
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