Assembly and micromanipulation of Xenopus in vitro-assembled mitotic chromosomes.

Sébastien Almagro, Stefan Dimitrov

Research output: Contribution to journalArticle

Abstract

Mitotic chromosomes have fascinated scientists for several decades. Despite the numerous microscopy studies, chromosome structure is, however, still poorly understood. This is due to both the high complexity of the mitotic chromosomes and the lack of other appropriate techniques suitable for studying their organization. This unit describes a novel physical approach based on measurements of mitotic chromosome elasticity. The elasticity properties are determined by the underlying structure, and knowledge of them has allowed a description of the organization of the mitotic chromosomes and critical analysis of the available models. In this unit, a detailed protocol for the measurements of the elastic response of in vitro assembled mitotic chromosomes in Xenopus egg extract is presented.

Original languageEnglish
JournalCurrent protocols in cell biology / editorial board, Juan S. Bonifacino ... [et al.]
VolumeChapter 22
Publication statusPublished - Apr 2005
Externally publishedYes

Fingerprint

Micromanipulation
Xenopus
Chromosomes
Elasticity
Chromosome Structures
Ovum
Microscopy
In Vitro Techniques

ASJC Scopus subject areas

  • Medicine(all)

Cite this

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