Arachidonic acid influences intracellular calcium handling in human osteoblasts

Laura Soldati, Annalisa Terranegra, Bruno Baggio, Rita Biasion, Teresa Arcidiacono, Giovanna Priante, Daniele Cusi, Giuseppe Vezzoli

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

The effect of arachidonic acid (AA) on intracellular Ca2+ concentration ([Ca2+]i) in human osteoblasts MG63 was studied. AA caused a concentration-dependent increase in [Ca2+]i, mainly due to inward Ca2+ transport from extracellular environment. Moreover, AA in Ca2+ -free medium produced a small, transient increase of [Ca2+]i, indicating that AA may also trigger Ca2+ release from intracellular stores. Because the [Ca2+]i response to AA was inhibited by the cyclooxygenase (COX) inhibitor indomethacin, we tested the effect of prostaglandins (PGs), products of COX pathway. PGs E1 and E2 caused an increase in [Ca2+]i, which, however, was far lower than that obtained with AA. The [Ca2+]i response to AA was not inhibited by nifedipine, suggesting that AA did not activate a voltage-dependent Ca2+ channel. Our results indicate that AA could modulate [Ca2+]i in MG63 human osteoblasts, where it may influence Ca2+ transport across both plasma and endoplasmic membranes. Furthermore, they suggest that osteoblast activity may be modulated by AA.

Original languageEnglish
Pages (from-to)91-96
Number of pages6
JournalProstaglandins Leukotrienes and Essential Fatty Acids
Volume75
Issue number2
DOIs
Publication statusPublished - Aug 2006
Externally publishedYes

Fingerprint

Osteoblasts
Arachidonic Acid
Calcium
Cyclooxygenase Inhibitors
Alprostadil
Nifedipine
Prostaglandin-Endoperoxide Synthases
Dinoprostone
Indomethacin
Prostaglandins
Cell Membrane
Membranes
Plasmas

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Endocrinology, Diabetes and Metabolism

Cite this

Arachidonic acid influences intracellular calcium handling in human osteoblasts. / Soldati, Laura; Terranegra, Annalisa; Baggio, Bruno; Biasion, Rita; Arcidiacono, Teresa; Priante, Giovanna; Cusi, Daniele; Vezzoli, Giuseppe.

In: Prostaglandins Leukotrienes and Essential Fatty Acids, Vol. 75, No. 2, 08.2006, p. 91-96.

Research output: Contribution to journalArticle

Soldati, Laura ; Terranegra, Annalisa ; Baggio, Bruno ; Biasion, Rita ; Arcidiacono, Teresa ; Priante, Giovanna ; Cusi, Daniele ; Vezzoli, Giuseppe. / Arachidonic acid influences intracellular calcium handling in human osteoblasts. In: Prostaglandins Leukotrienes and Essential Fatty Acids. 2006 ; Vol. 75, No. 2. pp. 91-96.
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