Arachidonic acid influences intracellular calcium handling in human osteoblasts

Laura Soldati, Annalisa Terranegra, Bruno Baggio, Rita Biasion, Teresa Arcidiacono, Giovanna Priante, Daniele Cusi, Giuseppe Vezzoli

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Abstract

The effect of arachidonic acid (AA) on intracellular Ca2+ concentration ([Ca2+]i) in human osteoblasts MG63 was studied. AA caused a concentration-dependent increase in [Ca2+]i, mainly due to inward Ca2+ transport from extracellular environment. Moreover, AA in Ca2+ -free medium produced a small, transient increase of [Ca2+]i, indicating that AA may also trigger Ca2+ release from intracellular stores. Because the [Ca2+]i response to AA was inhibited by the cyclooxygenase (COX) inhibitor indomethacin, we tested the effect of prostaglandins (PGs), products of COX pathway. PGs E1 and E2 caused an increase in [Ca2+]i, which, however, was far lower than that obtained with AA. The [Ca2+]i response to AA was not inhibited by nifedipine, suggesting that AA did not activate a voltage-dependent Ca2+ channel. Our results indicate that AA could modulate [Ca2+]i in MG63 human osteoblasts, where it may influence Ca2+ transport across both plasma and endoplasmic membranes. Furthermore, they suggest that osteoblast activity may be modulated by AA.

Original languageEnglish
Pages (from-to)91-96
Number of pages6
JournalProstaglandins Leukotrienes and Essential Fatty Acids
Volume75
Issue number2
DOIs
Publication statusPublished - Aug 2006
Externally publishedYes

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ASJC Scopus subject areas

  • Clinical Biochemistry
  • Endocrinology, Diabetes and Metabolism

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