Arachidonic acid increases intracellular calcium in erythrocytes

Laura Soldati, Cinzia Lombardi, Donatella Adamo, Annalisa Terranegra, Cristiana Bianchin, Giuseppe Bianchi, Giuseppe Vezzoli

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Recently, we have measured in erythrocytes a voltage-modulated and dihydropyridine-inhibited calcium influx. Since arachidonic acid and other polyunsaturated fatty acids influence the activities of most ion channels, we studied their effects on the erythrocyte Ca2+ influx. It was measured on fresh erythrocytes, isolated from healthy donors, using the fluorescent dye Fura 2 as indicator of [Ca2+]i. AA (5-50 μM) and EPA (20-30 μM) stimulated a concentration-dependent increase in [Ca2+]i, deriving from extracellular calcium (1 mM), without affecting the intra- and extracellular pH and membrane voltage. The Ca2+ influx rate varied from 0.5 to 3 nM Ca2+/s in the presence of AA and from 0.9 to 1.7 nM Ca2+/s with EPA. The Ca2+ influx elicited by AA and EPA was not inhibited by dihydropyridines, while cyclooxygenase inhibitors were effective and PGE1 or PGE2 did not produce any effect. We conclude that AA could activate an erythrocyte voltage-independent Ca2+ transport via an intermediate product of cyclooxygenase pathway; however, a direct interaction with the membrane lipid-protein cannot be excluded.

Original languageEnglish
Pages (from-to)974-978
Number of pages5
JournalBiochemical and Biophysical Research Communications
Volume293
Issue number3
DOIs
Publication statusPublished - 2002
Externally publishedYes

Fingerprint

Arachidonic Acid
Erythrocytes
Calcium
Electric potential
Dihydropyridines
Cyclooxygenase Inhibitors
Fura-2
Alprostadil
Prostaglandin-Endoperoxide Synthases
Membrane Lipids
Unsaturated Fatty Acids
Ion Channels
Fluorescent Dyes
Dinoprostone
Membranes
Membrane Proteins
Proteins

Keywords

  • Arachidonic acid
  • Ca influx
  • Cyclooxygenases
  • Erythrocytes
  • Fura 2
  • Polyunsaturated fatty acids
  • Prostaglandins

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Arachidonic acid increases intracellular calcium in erythrocytes. / Soldati, Laura; Lombardi, Cinzia; Adamo, Donatella; Terranegra, Annalisa; Bianchin, Cristiana; Bianchi, Giuseppe; Vezzoli, Giuseppe.

In: Biochemical and Biophysical Research Communications, Vol. 293, No. 3, 2002, p. 974-978.

Research output: Contribution to journalArticle

Soldati, Laura ; Lombardi, Cinzia ; Adamo, Donatella ; Terranegra, Annalisa ; Bianchin, Cristiana ; Bianchi, Giuseppe ; Vezzoli, Giuseppe. / Arachidonic acid increases intracellular calcium in erythrocytes. In: Biochemical and Biophysical Research Communications. 2002 ; Vol. 293, No. 3. pp. 974-978.
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