Application of luminescent Eu

Gd2O3 nanoparticles to the visualization of protein micropatterns

Dosi Dosev, Mikaela Nichkova, Maozi Liu, Bing Guo, Gang Yu Liu, Bruce D. Hammock, Ian M. Kennedy

Research output: Contribution to journalArticle

61 Citations (Scopus)

Abstract

Nanoparticle phosphors made of lanthanide oxides are a promising new class of tags in biochemistry because of their large Stokes shift, sharp emission spectra, long luminescence lifetime, and good photostability. We demonstrate the application of these nanoparticles to the visualization of protein micropatterns. Luminescent europium-doped gadolinium oxide (Eu:Gd 2O3) nanoparticles are synthesized by spray pyrolysis. The size distribution is from 5 to 200 nm. The particles are characterized by means of laserinduced fluorescent spectroscopy and transmission electron microscopy (TEM). The main emission peak is at 612 nm. The nanoparticles are coated with avidin through physical adsorption. biotinylated bovine serum albumin (BSA-b) is patterned on a silicon wafer using a microcontact printing technique. The wafer is then incubated in a solution of avidin-coated nanoparticles. Fluorescent microscopic images reveal that the nanoparticles are organized onto designated area, as defined by the microcontact printing process. The luminescent nanoparticles do not suffer photobleaching during the observation, which demonstrates their suitability as luminescent labels for fluorescence microscopy studies. More detailed studies are preformed using atomic-force microscopy (AFM) at a single nanoparticle level. The specific and the nonspecific binding densities of the particles are qualitatively evaluated.

Original languageEnglish
Article number064006
JournalJournal of Biomedical Optics
Volume10
Issue number6
DOIs
Publication statusPublished - Nov 2005
Externally publishedYes

Fingerprint

Nanoparticles
Visualization
proteins
Proteins
nanoparticles
Printing
Avidin
printing
wafers
Europium
Photobleaching
Lanthanoid Series Elements
biochemistry
Biochemistry
Oxides
Spray pyrolysis
oxides
Atomic Force Microscopy
Gadolinium
Fluorescence microscopy

Keywords

  • Biosensor
  • Lanthanide
  • Luminescence
  • Microcontact printing
  • Nanoparticles

ASJC Scopus subject areas

  • Biomedical Engineering
  • Biomaterials
  • Electronic, Optical and Magnetic Materials
  • Atomic and Molecular Physics, and Optics
  • Radiology Nuclear Medicine and imaging
  • Radiological and Ultrasound Technology
  • Clinical Biochemistry

Cite this

Application of luminescent Eu : Gd2O3 nanoparticles to the visualization of protein micropatterns. / Dosev, Dosi; Nichkova, Mikaela; Liu, Maozi; Guo, Bing; Liu, Gang Yu; Hammock, Bruce D.; Kennedy, Ian M.

In: Journal of Biomedical Optics, Vol. 10, No. 6, 064006, 11.2005.

Research output: Contribution to journalArticle

Dosev, Dosi ; Nichkova, Mikaela ; Liu, Maozi ; Guo, Bing ; Liu, Gang Yu ; Hammock, Bruce D. ; Kennedy, Ian M. / Application of luminescent Eu : Gd2O3 nanoparticles to the visualization of protein micropatterns. In: Journal of Biomedical Optics. 2005 ; Vol. 10, No. 6.
@article{870a71930aa2461b97613e56f2a5017d,
title = "Application of luminescent Eu: Gd2O3 nanoparticles to the visualization of protein micropatterns",
abstract = "Nanoparticle phosphors made of lanthanide oxides are a promising new class of tags in biochemistry because of their large Stokes shift, sharp emission spectra, long luminescence lifetime, and good photostability. We demonstrate the application of these nanoparticles to the visualization of protein micropatterns. Luminescent europium-doped gadolinium oxide (Eu:Gd 2O3) nanoparticles are synthesized by spray pyrolysis. The size distribution is from 5 to 200 nm. The particles are characterized by means of laserinduced fluorescent spectroscopy and transmission electron microscopy (TEM). The main emission peak is at 612 nm. The nanoparticles are coated with avidin through physical adsorption. biotinylated bovine serum albumin (BSA-b) is patterned on a silicon wafer using a microcontact printing technique. The wafer is then incubated in a solution of avidin-coated nanoparticles. Fluorescent microscopic images reveal that the nanoparticles are organized onto designated area, as defined by the microcontact printing process. The luminescent nanoparticles do not suffer photobleaching during the observation, which demonstrates their suitability as luminescent labels for fluorescence microscopy studies. More detailed studies are preformed using atomic-force microscopy (AFM) at a single nanoparticle level. The specific and the nonspecific binding densities of the particles are qualitatively evaluated.",
keywords = "Biosensor, Lanthanide, Luminescence, Microcontact printing, Nanoparticles",
author = "Dosi Dosev and Mikaela Nichkova and Maozi Liu and Bing Guo and Liu, {Gang Yu} and Hammock, {Bruce D.} and Kennedy, {Ian M.}",
year = "2005",
month = "11",
doi = "10.1117/1.2136347",
language = "English",
volume = "10",
journal = "Journal of Biomedical Optics",
issn = "1083-3668",
publisher = "SPIE",
number = "6",

}

TY - JOUR

T1 - Application of luminescent Eu

T2 - Gd2O3 nanoparticles to the visualization of protein micropatterns

AU - Dosev, Dosi

AU - Nichkova, Mikaela

AU - Liu, Maozi

AU - Guo, Bing

AU - Liu, Gang Yu

AU - Hammock, Bruce D.

AU - Kennedy, Ian M.

PY - 2005/11

Y1 - 2005/11

N2 - Nanoparticle phosphors made of lanthanide oxides are a promising new class of tags in biochemistry because of their large Stokes shift, sharp emission spectra, long luminescence lifetime, and good photostability. We demonstrate the application of these nanoparticles to the visualization of protein micropatterns. Luminescent europium-doped gadolinium oxide (Eu:Gd 2O3) nanoparticles are synthesized by spray pyrolysis. The size distribution is from 5 to 200 nm. The particles are characterized by means of laserinduced fluorescent spectroscopy and transmission electron microscopy (TEM). The main emission peak is at 612 nm. The nanoparticles are coated with avidin through physical adsorption. biotinylated bovine serum albumin (BSA-b) is patterned on a silicon wafer using a microcontact printing technique. The wafer is then incubated in a solution of avidin-coated nanoparticles. Fluorescent microscopic images reveal that the nanoparticles are organized onto designated area, as defined by the microcontact printing process. The luminescent nanoparticles do not suffer photobleaching during the observation, which demonstrates their suitability as luminescent labels for fluorescence microscopy studies. More detailed studies are preformed using atomic-force microscopy (AFM) at a single nanoparticle level. The specific and the nonspecific binding densities of the particles are qualitatively evaluated.

AB - Nanoparticle phosphors made of lanthanide oxides are a promising new class of tags in biochemistry because of their large Stokes shift, sharp emission spectra, long luminescence lifetime, and good photostability. We demonstrate the application of these nanoparticles to the visualization of protein micropatterns. Luminescent europium-doped gadolinium oxide (Eu:Gd 2O3) nanoparticles are synthesized by spray pyrolysis. The size distribution is from 5 to 200 nm. The particles are characterized by means of laserinduced fluorescent spectroscopy and transmission electron microscopy (TEM). The main emission peak is at 612 nm. The nanoparticles are coated with avidin through physical adsorption. biotinylated bovine serum albumin (BSA-b) is patterned on a silicon wafer using a microcontact printing technique. The wafer is then incubated in a solution of avidin-coated nanoparticles. Fluorescent microscopic images reveal that the nanoparticles are organized onto designated area, as defined by the microcontact printing process. The luminescent nanoparticles do not suffer photobleaching during the observation, which demonstrates their suitability as luminescent labels for fluorescence microscopy studies. More detailed studies are preformed using atomic-force microscopy (AFM) at a single nanoparticle level. The specific and the nonspecific binding densities of the particles are qualitatively evaluated.

KW - Biosensor

KW - Lanthanide

KW - Luminescence

KW - Microcontact printing

KW - Nanoparticles

UR - http://www.scopus.com/inward/record.url?scp=33645216972&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33645216972&partnerID=8YFLogxK

U2 - 10.1117/1.2136347

DO - 10.1117/1.2136347

M3 - Article

VL - 10

JO - Journal of Biomedical Optics

JF - Journal of Biomedical Optics

SN - 1083-3668

IS - 6

M1 - 064006

ER -