Application of FTIR and LA-ICPMS spectroscopies as a possible approach for biochemical analyses of different rat brain regions

Mohamed Hosni M. Ali, Fazle Rakib, Volker Nischwitz, Ehsan Ullah, RaghvenPhDa Mall, Amjad M. Shraim, M. I. Ahmad, Zafar Khan Ghouri, Donald McNaughton, Stephan Küppers, Tariq Ahmed, Khalid Al-Saad

Research output: Contribution to journalArticle

Abstract

Fourier Transform Infrared Spectroscopy (FTIR) is a non-destructive analytical technique that has been employed in this research to characterize the biochemical make-up of various rat brain regions. The sensorimotor cortex, caudate putamen, thalamus, and the hippocampus were found to have higher olefinic content-an indicator of a higher degree of unsaturated fatty acids-rich in short-chain fatty acids, and low in ester and lipid contents. While the regions of the corpus callosum, internal, and external capsule were found to contain long-chained and higher-esterified saturated fatty acids. These molecular differences may reflect the roles of the specific regions in information processing and can provide a unique biochemical platform for future studies on the earlier detection of pathology development in the brain, as a consequence of disease or injury. Laser Ablation Inductively Coupled Plasma Mass Spectroscopy (LA-ICP-MS) is another vital analytical technique that was used in this work to analyze the elements' distribution patterns in various regions of the brain. The complementary data sets allowed the characterization of the brain regions, the chemical dominating groups, and the elemental composition. This set-up may be used for the investigation of changes in the brain caused by diseases and help create a deeper understanding of the interactions between the organic and elemental composition.

Original languageEnglish
Article number2436
JournalApplied Sciences (Switzerland)
Volume8
Issue number12
DOIs
Publication statusPublished - 1 Dec 2018

Fingerprint

rats
brain
Fourier transform infrared spectroscopy
Rats
Brain
infrared spectroscopy
Spectroscopy
fatty acids
spectroscopy
thalamus
Saturated fatty acids
hippocampus
Unsaturated fatty acids
Volatile Fatty Acids
distribution (property)
cortexes
pathology
Inductively coupled plasma
Pathology
Laser ablation

Keywords

  • Biochemical analysis
  • Brain
  • FTIR imaging spectroscopy
  • LA-ICP-MS

ASJC Scopus subject areas

  • Materials Science(all)
  • Instrumentation
  • Engineering(all)
  • Process Chemistry and Technology
  • Computer Science Applications
  • Fluid Flow and Transfer Processes

Cite this

Application of FTIR and LA-ICPMS spectroscopies as a possible approach for biochemical analyses of different rat brain regions. / Hosni M. Ali, Mohamed; Rakib, Fazle; Nischwitz, Volker; Ullah, Ehsan; Mall, RaghvenPhDa; Shraim, Amjad M.; Ahmad, M. I.; Ghouri, Zafar Khan; McNaughton, Donald; Küppers, Stephan; Ahmed, Tariq; Al-Saad, Khalid.

In: Applied Sciences (Switzerland), Vol. 8, No. 12, 2436, 01.12.2018.

Research output: Contribution to journalArticle

Hosni M. Ali, Mohamed ; Rakib, Fazle ; Nischwitz, Volker ; Ullah, Ehsan ; Mall, RaghvenPhDa ; Shraim, Amjad M. ; Ahmad, M. I. ; Ghouri, Zafar Khan ; McNaughton, Donald ; Küppers, Stephan ; Ahmed, Tariq ; Al-Saad, Khalid. / Application of FTIR and LA-ICPMS spectroscopies as a possible approach for biochemical analyses of different rat brain regions. In: Applied Sciences (Switzerland). 2018 ; Vol. 8, No. 12.
@article{16acdeb2c58f4014b424f1eb97fbd077,
title = "Application of FTIR and LA-ICPMS spectroscopies as a possible approach for biochemical analyses of different rat brain regions",
abstract = "Fourier Transform Infrared Spectroscopy (FTIR) is a non-destructive analytical technique that has been employed in this research to characterize the biochemical make-up of various rat brain regions. The sensorimotor cortex, caudate putamen, thalamus, and the hippocampus were found to have higher olefinic content-an indicator of a higher degree of unsaturated fatty acids-rich in short-chain fatty acids, and low in ester and lipid contents. While the regions of the corpus callosum, internal, and external capsule were found to contain long-chained and higher-esterified saturated fatty acids. These molecular differences may reflect the roles of the specific regions in information processing and can provide a unique biochemical platform for future studies on the earlier detection of pathology development in the brain, as a consequence of disease or injury. Laser Ablation Inductively Coupled Plasma Mass Spectroscopy (LA-ICP-MS) is another vital analytical technique that was used in this work to analyze the elements' distribution patterns in various regions of the brain. The complementary data sets allowed the characterization of the brain regions, the chemical dominating groups, and the elemental composition. This set-up may be used for the investigation of changes in the brain caused by diseases and help create a deeper understanding of the interactions between the organic and elemental composition.",
keywords = "Biochemical analysis, Brain, FTIR imaging spectroscopy, LA-ICP-MS",
author = "{Hosni M. Ali}, Mohamed and Fazle Rakib and Volker Nischwitz and Ehsan Ullah and RaghvenPhDa Mall and Shraim, {Amjad M.} and Ahmad, {M. I.} and Ghouri, {Zafar Khan} and Donald McNaughton and Stephan K{\"u}ppers and Tariq Ahmed and Khalid Al-Saad",
year = "2018",
month = "12",
day = "1",
doi = "10.3390/app8122436",
language = "English",
volume = "8",
journal = "Applied Sciences (Switzerland)",
issn = "2076-3417",
publisher = "Multidisciplinary Digital Publishing Institute (MDPI)",
number = "12",

}

TY - JOUR

T1 - Application of FTIR and LA-ICPMS spectroscopies as a possible approach for biochemical analyses of different rat brain regions

AU - Hosni M. Ali, Mohamed

AU - Rakib, Fazle

AU - Nischwitz, Volker

AU - Ullah, Ehsan

AU - Mall, RaghvenPhDa

AU - Shraim, Amjad M.

AU - Ahmad, M. I.

AU - Ghouri, Zafar Khan

AU - McNaughton, Donald

AU - Küppers, Stephan

AU - Ahmed, Tariq

AU - Al-Saad, Khalid

PY - 2018/12/1

Y1 - 2018/12/1

N2 - Fourier Transform Infrared Spectroscopy (FTIR) is a non-destructive analytical technique that has been employed in this research to characterize the biochemical make-up of various rat brain regions. The sensorimotor cortex, caudate putamen, thalamus, and the hippocampus were found to have higher olefinic content-an indicator of a higher degree of unsaturated fatty acids-rich in short-chain fatty acids, and low in ester and lipid contents. While the regions of the corpus callosum, internal, and external capsule were found to contain long-chained and higher-esterified saturated fatty acids. These molecular differences may reflect the roles of the specific regions in information processing and can provide a unique biochemical platform for future studies on the earlier detection of pathology development in the brain, as a consequence of disease or injury. Laser Ablation Inductively Coupled Plasma Mass Spectroscopy (LA-ICP-MS) is another vital analytical technique that was used in this work to analyze the elements' distribution patterns in various regions of the brain. The complementary data sets allowed the characterization of the brain regions, the chemical dominating groups, and the elemental composition. This set-up may be used for the investigation of changes in the brain caused by diseases and help create a deeper understanding of the interactions between the organic and elemental composition.

AB - Fourier Transform Infrared Spectroscopy (FTIR) is a non-destructive analytical technique that has been employed in this research to characterize the biochemical make-up of various rat brain regions. The sensorimotor cortex, caudate putamen, thalamus, and the hippocampus were found to have higher olefinic content-an indicator of a higher degree of unsaturated fatty acids-rich in short-chain fatty acids, and low in ester and lipid contents. While the regions of the corpus callosum, internal, and external capsule were found to contain long-chained and higher-esterified saturated fatty acids. These molecular differences may reflect the roles of the specific regions in information processing and can provide a unique biochemical platform for future studies on the earlier detection of pathology development in the brain, as a consequence of disease or injury. Laser Ablation Inductively Coupled Plasma Mass Spectroscopy (LA-ICP-MS) is another vital analytical technique that was used in this work to analyze the elements' distribution patterns in various regions of the brain. The complementary data sets allowed the characterization of the brain regions, the chemical dominating groups, and the elemental composition. This set-up may be used for the investigation of changes in the brain caused by diseases and help create a deeper understanding of the interactions between the organic and elemental composition.

KW - Biochemical analysis

KW - Brain

KW - FTIR imaging spectroscopy

KW - LA-ICP-MS

UR - http://www.scopus.com/inward/record.url?scp=85057866713&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85057866713&partnerID=8YFLogxK

U2 - 10.3390/app8122436

DO - 10.3390/app8122436

M3 - Article

VL - 8

JO - Applied Sciences (Switzerland)

JF - Applied Sciences (Switzerland)

SN - 2076-3417

IS - 12

M1 - 2436

ER -