Application of fluorescent Eu: Gd 2O 3 nanoparticles to the visualization of protein micropatterns

Dosi Dosev, Mikaela Nichkova, Maozi Liu, Bing Guo, Gang Yu Liu, Younan Xia, Bruce D. Hammock, Ian M. Kennedy

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Nanoparticles made of lanthanide oxides are promising fluorophores as a new class of tags in biochemistry because of their large Stokes shift, sharp emission spectra, long lifetime and lack of photobleaching. We demonstrate for first time the application of these nanoparticles to the visualization of protein micropatterns. Europium-doped gadolinium oxide (Eu:Gd 2O 3) nanoparticles were synthesized by spray pyrolysis and were characterized by means of laser-induced fluorescent spectroscopy and TEM. Their main emission peak is at 612 nm. And their size distribution is from 5 nm to 500 nm. The nanoparticles were coated with avidin through physical adsorption. Biotinylated Bovine Serum Albumin (BSA-b) was patterned on a silicon wafer using a micro-contact printing technique. The BSA-b - patterned wafer was incubated in a solution containing the avidin-coated nanoparticles. The specific interaction between biotin and avidin was studied by means of fluorescent microscopy and atomic-force microscopy (AFM). The fluorescent microscopic images revealed that the nanoparticles were organized into designated structures as defined by the microcontact printing process - non-specific binding of the avidin-coated nanoparticles to bare substrate was negligible. The fluorescent pattern did not suffer any photobleaching during the observation process which demonstrates the suitability of Eu:Gd 2O 3 nanoparticles as fluorescent labels with extended excitation periods - organic dyes, including chelates, suffer bleaching over the same period. More detailed studies were preformed using AFM at a single nanoparticle level. The specific and the non-specific binding densities of the particles were qualitatively evaluated.

Original languageEnglish
Article number84
Pages (from-to)473-481
Number of pages9
JournalProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume5699
DOIs
Publication statusPublished - 2005
Externally publishedYes

Fingerprint

Nanoparticles
Visualization
proteins
Proteins
nanoparticles
Avidin
Photobleaching
Printing
Atomic Force Microscopy
Bovine Serum Albumin
albumins
printing
serums
Atomic force microscopy
atomic force microscopy
wafers
Europium
Lanthanoid Series Elements
biochemistry
biotin

Keywords

  • AFM
  • Biosensor
  • Fluorescence
  • Lanthanide
  • Microcontact printing
  • Nanoparticles

ASJC Scopus subject areas

  • Engineering(all)

Cite this

Application of fluorescent Eu : Gd 2O 3 nanoparticles to the visualization of protein micropatterns. / Dosev, Dosi; Nichkova, Mikaela; Liu, Maozi; Guo, Bing; Liu, Gang Yu; Xia, Younan; Hammock, Bruce D.; Kennedy, Ian M.

In: Progress in Biomedical Optics and Imaging - Proceedings of SPIE, Vol. 5699, 84, 2005, p. 473-481.

Research output: Contribution to journalArticle

Dosev, Dosi ; Nichkova, Mikaela ; Liu, Maozi ; Guo, Bing ; Liu, Gang Yu ; Xia, Younan ; Hammock, Bruce D. ; Kennedy, Ian M. / Application of fluorescent Eu : Gd 2O 3 nanoparticles to the visualization of protein micropatterns. In: Progress in Biomedical Optics and Imaging - Proceedings of SPIE. 2005 ; Vol. 5699. pp. 473-481.
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