Apple messenger RNAs related to bacterial lignostilbene dioxygenase and plant SAUR genes are preferentially expressed in flowers

Bernard Watillon, Richard Kettmann, Abdelilah Arredouani, Jean François Hecquet, Philippe Boxus, Arsène Burny

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

In an attempt to use a differential display procedure to identify organ-specific genes in apple, cDNA fragments of two transcripts preferentially expressed in flowers were isolated and corresponding full-length cDNA inserts were subsequently obtained. One of these clones, Md-FS1, belongs to the SAUR gene family, originally identified as a set of auxin-inducible genes in soybean. The second one, Md-FS2, encodes a polypeptide with sequence similarities to bacterial lignostilbene-α,β-dioxygenase isozymes, which are thought to be involved in lignin biodegradation. Northern blot analysis confirmed that both genes are preferentially expressed in floral organs at full bloom, while being expressed at a lower or undetectable levels in vegetative organs (leaves, shoots or roots) as well as in immature, green and unopened blossoms. Furthermore, Md-FS1 transcripts also appeared to accumulate in vegetative tissues after auxin treatment of micropropagated apple shoots.

Original languageEnglish
Pages (from-to)909-915
Number of pages7
JournalPlant Molecular Biology
Volume36
Issue number6
DOIs
Publication statusPublished - 1 Apr 1998
Externally publishedYes

Fingerprint

Dioxygenases
Plant Genes
Malus
messenger RNA
apples
Genes
flowers
Messenger RNA
Indoleacetic Acids
auxins
genes
Complementary DNA
shoots
Lignin
Biodegradation
biodegradation
Soybeans
Northern blotting
Northern Blotting
Isoenzymes

Keywords

  • Apple
  • Differential display
  • Lignostilbene dioxygenase
  • Malus
  • SAUR genes

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry

Cite this

Apple messenger RNAs related to bacterial lignostilbene dioxygenase and plant SAUR genes are preferentially expressed in flowers. / Watillon, Bernard; Kettmann, Richard; Arredouani, Abdelilah; Hecquet, Jean François; Boxus, Philippe; Burny, Arsène.

In: Plant Molecular Biology, Vol. 36, No. 6, 01.04.1998, p. 909-915.

Research output: Contribution to journalArticle

Watillon, Bernard ; Kettmann, Richard ; Arredouani, Abdelilah ; Hecquet, Jean François ; Boxus, Philippe ; Burny, Arsène. / Apple messenger RNAs related to bacterial lignostilbene dioxygenase and plant SAUR genes are preferentially expressed in flowers. In: Plant Molecular Biology. 1998 ; Vol. 36, No. 6. pp. 909-915.
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