Apolipoprotein A1 -75 G/A and +83 C/T polymorphisms: Susceptibility and prognostic implications in breast cancer

Bechr Hamrita, Hela Ben Nasr, Sallouha Gabbouj, Noureddine Bouaouina, Lotfi Chouchane, Karim Chahed

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19 Citations (Scopus)

Abstract

Apolipoprotein A1 (ApoA1) is the major apoprotein constituent of high-density lipoprotein that can play important roles in tumor invasion and metastasis. In the current report, we evaluated the role of the functional ApoA1 polymorphisms (-75 G/A and +83 C/T) as genetic markers for breast cancer susceptibility and prognosis. We used the polymerase chain reaction and restriction enzyme digestion (RFLP-PCR) to characterize the variations of the ApoA1 gene in 295 unrelated Tunisian patients with breast carcinoma and 197 healthy control subjects. No association was found between the +83 C/T genetic variation in ApoA1 gene and the risk of breast cancer occurrence. The presence of the (+83) T allele appeared however to be associated with an increased risk of lymph node metastasis occurrence (OR = 2.94; P = 0.01). Furthermore, a positive association was found between ApoA1 -75 A allele carriers and breast cancer risk (OR = 1.57; P = 0.02). Regarding prognostic indicators, a significant association was found between ApoA1 (-75) A allele carriers and the premenopausal status of breast cancer patients (OR = 1.73; P = 0.03). Additionally, the presence of the -75 A allele was correlated with the oestrogen receptor status among premenopausal women (OR = 2.45; P = 0.02). This is the first report on the studies of ApoA1 single nucleotide polymorphisms (SNPs) in breast carcinomas. Our data suggest that these genetic variations of ApoA1 may represent a marker for the increased risk of breast cancer.

Original languageEnglish
Pages (from-to)1637-1643
Number of pages7
JournalMolecular Biology Reports
Volume38
Issue number3
DOIs
Publication statusPublished - Mar 2011

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Keywords

  • Apolipoprotein A1
  • Breast carcinoma
  • Polymorphism
  • Prognosis
  • Susceptibility

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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