Apolipoprotein A1 -75 G/A and +83 C/T polymorphisms

Susceptibility and prognostic implications in breast cancer

Bechr Hamrita, Hela Ben Nasr, Sallouha Gabbouj, Noureddine Bouaouina, Lotfi Chouchane, Karim Chahed

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Apolipoprotein A1 (ApoA1) is the major apoprotein constituent of high-density lipoprotein that can play important roles in tumor invasion and metastasis. In the current report, we evaluated the role of the functional ApoA1 polymorphisms (-75 G/A and +83 C/T) as genetic markers for breast cancer susceptibility and prognosis. We used the polymerase chain reaction and restriction enzyme digestion (RFLP-PCR) to characterize the variations of the ApoA1 gene in 295 unrelated Tunisian patients with breast carcinoma and 197 healthy control subjects. No association was found between the +83 C/T genetic variation in ApoA1 gene and the risk of breast cancer occurrence. The presence of the (+83) T allele appeared however to be associated with an increased risk of lymph node metastasis occurrence (OR = 2.94; P = 0.01). Furthermore, a positive association was found between ApoA1 -75 A allele carriers and breast cancer risk (OR = 1.57; P = 0.02). Regarding prognostic indicators, a significant association was found between ApoA1 (-75) A allele carriers and the premenopausal status of breast cancer patients (OR = 1.73; P = 0.03). Additionally, the presence of the -75 A allele was correlated with the oestrogen receptor status among premenopausal women (OR = 2.45; P = 0.02). This is the first report on the studies of ApoA1 single nucleotide polymorphisms (SNPs) in breast carcinomas. Our data suggest that these genetic variations of ApoA1 may represent a marker for the increased risk of breast cancer.

Original languageEnglish
Pages (from-to)1637-1643
Number of pages7
JournalMolecular Biology Reports
Volume38
Issue number3
DOIs
Publication statusPublished - Mar 2011

Fingerprint

Apolipoproteins
Apolipoprotein A-I
Breast Neoplasms
Alleles
Neoplasm Metastasis
Polymerase Chain Reaction
Apoproteins
HDL Lipoproteins
Genetic Markers
Restriction Fragment Length Polymorphisms
Estrogen Receptors
Genes
Single Nucleotide Polymorphism
Digestion
Healthy Volunteers
Lymph Nodes
Enzymes
Neoplasms

Keywords

  • Apolipoprotein A1
  • Breast carcinoma
  • Polymorphism
  • Prognosis
  • Susceptibility

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Apolipoprotein A1 -75 G/A and +83 C/T polymorphisms : Susceptibility and prognostic implications in breast cancer. / Hamrita, Bechr; Nasr, Hela Ben; Gabbouj, Sallouha; Bouaouina, Noureddine; Chouchane, Lotfi; Chahed, Karim.

In: Molecular Biology Reports, Vol. 38, No. 3, 03.2011, p. 1637-1643.

Research output: Contribution to journalArticle

Hamrita, Bechr ; Nasr, Hela Ben ; Gabbouj, Sallouha ; Bouaouina, Noureddine ; Chouchane, Lotfi ; Chahed, Karim. / Apolipoprotein A1 -75 G/A and +83 C/T polymorphisms : Susceptibility and prognostic implications in breast cancer. In: Molecular Biology Reports. 2011 ; Vol. 38, No. 3. pp. 1637-1643.
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